Artırılmış Biyoaktivite için Bazı Flavonoid Karışımlarının Elektroeğirme Tekniği Kullanılarak Enkapsülasyonu ve Kontrollü Salım Çalışmaları

Yıl 2025, Cilt: 7 Sayı: 1, 141 - 152, 30.04.2025

Deniz Tuğçe Algan , Erdal Kocabaş

Öz

Narenciye meyvelerinden elde edilen biyoaktif bileşikler olan rutin hidrat, naringin ve hesperidin kullanılarak polimer bazlı elektroeğirme fiberleri hazırlanmıştır. Bu flavonoidlerin antioksidan, anti-inflamatuar ve anti-kanser gibi çeşitli biyolojik aktiviteleri bulunmaktadır. Elektroeğirme tekniği sayesinde, flavonoidlerin biyolojik kullanılabilirliği artırılarak, ilaç taşıma sistemleri, gıda ambalajları ve kozmetik ürünler gibi farklı alanlarda potansiyel uygulamalar geliştirilmesi amaçlanmıştır. Elde edilen fiberlerin morfolojik ve kimyasal ve karakterizasyonları ile flavonoidlerin fiber matris içerisindeki dağılımı ve salım davranışları belirlenmiştir. Aynı zamanda, nanofiberlerin Staphylococcus aureus (S. aureus) bakterisine karşı antibakteriyel aktivitesi değerlendirildi. Yapılan çalışmanın sonucunda bağırsak pH’ında (pH 7,4) yapılan dissolüsyon analizinde PLA-MIX fiberi etken maddeleri %20-25 oranında, PCL- MIX fiberi etken maddeleri %21-28 oranında salındı. Antibakteriyel sonuçlar nanofiberlerin %45 oranında PLA karışımında daha fazla bakteriyel inhibisyona neden olduğunu gösterdi. Bu çalışma, narenciye atıklarının değerlendirilmesi ve yeni nesil biyomalzemelerin geliştirilmesi açısından önemli bir katkı sağlamaktadır.

Anahtar Kelimeler

Antibakteriyel etki , Elektroeğirme , Flavonoid , Hesperidin , in vitro ilaç salım , Naringin , Rutin hidrat

Etik Beyan

Bu makale, 6. Uluslararası Biyosensörler Kongresi'nde bir poster olarak sunulan “Polymer-Based Encapsulation of Flavonoids via Electrospinning for Nutraceutical Applications” başlıklı yayınlanmamış konferans sunumunun gözden geçirilmiş ve geliştirilmiş versiyonudur.

Destekleyen Kurum

Tübitak ve Necmettin Erbakan Üniversitesi

Proje Numarası

119C100 and NEÜBAP-221415002

Teşekkür

Bu çalışmada antibakteriyel aktivite çalışmalarına katkı sağlayan Necmettin Erbakan Üniversitesi Fen Fakültesi Biyoteknoloji Bölümünden Doç. Dr. Fatih Erci ve yüksek lisans öğrencisi Hala Boubaker'e teşekkür ediyorum.

Encapsulation of Some Flavonoid Mixtures Using Electrospinning Technique for Enhanced Bioactivity and Controlled Release Studies

Öz

Polymer-based electrospun fibers were prepared using bioactive compounds derived from citrus fruits such as rutin hydrate, naringin and hesperidin. These flavonoids have various biological activities such as antioxidant, anti-inflammatory and anticancer. By increasing the bioavailability of flavonoids through the electrospinning technique, it is aimed to develop potential applications in different areas such as drug delivery systems, food packaging and cosmetic products. The distribution and release behaviors of flavonoids in the fiber matrix were determined by morphological and chemical characterization of the obtained fibers. At the same time, the antibacterial activity of nanofibers against Staphylococcus aureus (S. aureus) bacteria was evaluated. As a result of the study, in the dissolution analysis performed at intestinal pH (pH 7.4), the active ingredients of PLA-MIX fiber were released at a rate of 20-25% and PCL-MIX fiber at a rate of 21-28%. Antibacterial results showed that nanofibers caused more bacterial inhibition in a 45% PLA mixture. This study provides an important contribution to the evaluation of citrus wastes and the development of new-generation biomaterials.

Anahtar Kelimeler

Antibacterial effect , Electrospinning , Flavonoid , Hesperidin , in vitro drug release , Naringin , Rutin hydrate

Etik Beyan

This article is the revised and developed version of the unpublished conference presentation entitled “Polymer-Based Encapsulation of Flavonoids via Electrospinning for Nutraceutical Applications," presented as a poster at the 6th International Congress on Biosensors.

Destekleyen Kurum

TÜBİTAK and Necmettin Erbakan University

Proje Numarası

119C100 and NEÜBAP-221415002

Teşekkür

I would like to thank Assoc. Prof. Dr. Fatih Erci and masters student Hala Boubaker, members of the Biotechnology Department of the Faculty of Science at Necmettin Erbakan University, for antibacterial activity assay experiments.

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