Rhynchites bacchus L. (Coleoptera: Rhynchitidae)’dan İzole Edilen Beauveria bassiana’nın Moleküler Karakterizasyonu ve Patojenitesi

Öz

Yaprak kıvrım böceği (Rhynchites bacchus L., Coleoptera: Rhynchitidae) hemen hemen bütün Dünya’da elma, erik, kayısı, kiraz ve şeftali (nektarin) gibi pek çok meyvenin en önemli zararlılarından birisidir. Bu zararlı hem taş hem de yumuşak çekirdekli meyvelerle beslenir ve meyve bahçelerinde %80-90’a kadar zarara neden olabilirler. Bu çalışmada, daha etkili ve güvenli biyolojik mücadele etmeni bulmak açısından bu zararlının fungal patojenleri araştırılmıştır. Böcek örnekleri Türkiye’nin Gümüşhane ilinde 2007 yılının Temmuz ayında R. bacchus ile istila edilmiş elma bahçelerinden toplanmıştır. Bir fungal örnek izole edilmiş ve şu anda kullanılan morfolojik ve ITS, EF1-σ kısmi sekansını içeren moleküler teknikler kullanılarak karakterize edilmiştir. Bu tekniklere dayanarak, bu izolat Beauveria bassiana olarak tanımlanmıştır. Ayrıca bu izolatın R. bacchus’un erginleri üzerindeki insektisidal aktivitesi belirlenmiştir. Fungus 1 × 108 mL-1spor konsantrasyonunun uygulanmasından sonra iki hafta sonunda %100 ölüme neden olmuştur ve bu fungusun LT50 değeri 7,5 gün olarak hesaplanmıştır. Bu çalışma R. bacchus’a karşı B. bassiana’nın etkinliğinin belirlenmesine yönelik ilk çalışmadır

Anahtar Kelimeler

• Yaprak kıvrım böceği, Beauveria bassiana, mikrobiyal mücadele

Molecular Characterization and Pathogenicity of Beauveria bassiana Isolated from Rhynchites bacchus L. (Coleoptera: Rhynchitidae)

Öz

The leafroller weevil beetle (Rhynchites bacchus L., Coleoptera: Rhynchitidae) is one of the most destructive pest of various fruits such as apple, plum, apricot, cherry and peach (nectarine) etc. almost all over the world. This pest feeds either stone or pome fruits and can cause up to 80-90% damage to orchards. In this study, we investigated fungal pathogens of this pest to find more effective and safe biological control agent against it. Insect specimens were collected from apple orchards invaded by R. baccus during July of 2007 in Gümüşhane, Turkey. One fungal species was isolated and characterized by currently used morphological and molecular techniques including ITS and the partial EF1-α sequencing. Based on these characteristics, the isolate was identified as Beauveria bassiana. Separately, the insecticidal activity of the isolate was determined on R. bacchus adults. The fungus caused 100% mortality within two weeks days after application of 1 × 10⁸ ml^-1 spore concentration and the LT₅₀ value of the fungus was calculated as 7.5 day. This is the first study to determine the effectiveness of B. bassiana against R. baccus.

Anahtar Kelimeler

• The leafroller weevil beetle, Beauveria bassiana, microbial control

Kaynakça

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