ŞEFTALİ POSASINDAN ULTRASON VE MİKRODALGA DESTEKLİ EKSTRAKSİYON YÖNTEMLERİYLE FENOLİK BİLEŞİKLERİN ELDESİ

Yıl 2019, , 875 - 881, 31.07.2019

Hande Baltacıoğlu , Emine Melike Şahin Ecem Duygu Karadağ

https://doi.org/10.28948/ngumuh.570250

Cited By: 4

Öz

   Bu çalışmada şeftali posasından fenolik
maddelerin ekstraksiyonu üzerine 3 farklı yöntemin etkisi toplam fenolik madde
(TFM) miktarları ve antioksidan aktiviteleri kıyaslanarak belirlenmiştir. Posadan
fenolik bileşikler % 80’lik metanol (% 1 HCl içeren) kullanılarak, geleneksel
yöntemle (30, 60, 120 ve 240 dakika); ultrason destekli ekstraksiyonla (% 60,
80 ve 100 genlik; 10, 20 ve 30 dakika); mikrodalga destekli ekstraksiyonla
(360, 600 ve 900 W; 30, 60 ve 90 saniye) uygulanarak ekstrakte edilmiştir. Geleneksel
yöntemde 4 saat sonunda TFM 1834,88±84,55 mg GAE/kg YA olarak belirlenmiştir. Ultrason
destekli ekstraksiyonda TFM %100 genlikte 10 dakika için 1817,21± 36,95 mg
GAE/kg YA olarak ölçülmüştür. Mikrodalga destekli ekstraksiyonda TFM, 900 W ve
90 s için 2802,464±61,49 mg GAE/kg YA olarak belirlenmiştir. EC₅₀
değeri, geleneksel ekstraksiyon için 37,54±0,08 mg/ml, ultrason destekli
ekstraksiyon için 73,72±0,13 mg/ml ve mikrodalga destekli ekstraksiyon için 63,17±0,16 mg/ml olarak belirlenmiştir. Buna
göre mikrodalga ve ultrason destekli ektraksiyonun zaman tasarrufu açısından
önemli avantajlar sağladığı söylenebilmektedir.

Anahtar Kelimeler

Şeftali, Fenolik Bileşikler, Ultrason Destekli Ekstraksiyon, Mikrodalga Destekli Ekstraksiyon

EXTRACTION OF PHENOLIC COMPOUNDS FROM PEACH POMACE BY MICROWAVE AND ULTRASOUND ASSISTED EXTRACTION

Öz

   In this study, the effect of three different
methods on the extraction of phenolic compounds from peach pomace was
determined in terms of total phenolic compound (TPC) and antioxidant activity.
Phenolic compounds were extracted from peach pomace using 80% methanol
(containing 1% HCl) by conventional method (30, 60, 120 and 240 minutes);
ultrasound-assisted extraction (60, 80 and 100% amplitude; 10, 20 and 30 min);
microwave-assisted extraction (360, 600 and 900 W; 30, 60 and 90 seconds). In
the conventional method, TPC was determined as 1834.88±84.55 mg GAE/kg FW after
4 hours. In the ultrasound-assisted extraction, TPC was measured as 1817.21 ±
36.95 mg GAE/kg FW at 100% amplitude for 10 minutes. It was determined that TPC
was 2802.464 ± 61.49 mg GAE/kg FW for microwave assisted extraction at 900 W
and 90 s. The EC₅₀ value was 37.54 ± 0.08 mg/ml for conventional
extraction, 73.72 ± 0.13 mg/ml for ultrasound assisted extraction and 63.17 ±
0.16 mg/ml for microwave assisted extraction. Accordingly, it can be said that
microwave and ultrasound assisted extraction provides significant advantages in
terms of time saving.

Anahtar Kelimeler

Phenolic compounds, microwave assisted extraction, peach, ultrasound assisted extraction

Kaynakça

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