Üç Boyutlu Hücre Kültürü Sistemlerine Güncel Yaklaşımlar

Elif Polat

doi:10.37696/nkmj.679069

Üç Boyutlu Hücre Kültürü Sistemlerine Güncel Yaklaşımlar

Öz

İki boyutlu (2B) hücre kültürü, hücre temelli araştırmalar için değerli bir yöntemdir ancak in vivo yanıtlar hakkında öngörülemeyen, yanıltıcı veriler sağlayabilir. Son yirmi yılda, hücresel mikro çevrenin (örneğin hücre dışı matris ve interstisyel sıvı) önemine farkındalık artmıştır. 3B hücre kültürü olarak adlandırılan bu yeni hücre kültürü paradigması hızla popülerlik kazanıyor. 2B'den 3B kültür tekniklerine geçiş, fizyolojik olarak daha uygun doku modellerine doğru önemli bir adımdır. 3B hücre kültürleri farklı amaçlar için faklı teknikler sunar ve gereksinime göre kullanıcıların en uygun modeli seçmeleri gerekir. 3B hücre kültürü sistemleri, kök hücre çalışmaları, ilaç keşifleri, kanser araştırmaları, gen ve protein ifade çalışmaları ve daha birçok karmaşık fizyolojik mekanizmanın aydınlatılabilmesi için bu alanlarda kullanıldığı görülmektedir. İn vitro çalışmalar; 3B hücre kültürlerinin ortaya çıkmasıyla, 2B kültürler ile mümkün olmayan karmaşık etkileşimleri incelemek için üstün yapılar sunarlar.

Anahtar Kelimeler

Üç boyutlu hücre kültürü,sferoid,skafold,teknikler

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Klinik Tıp Bilimleri

Bölüm

Derleme

Yazarlar

Elif Polat ^*
0000-0001-6808-5467
Türkiye

Yayımlanma Tarihi

20 Nisan 2020

Gönderilme Tarihi

23 Ocak 2020

Kabul Tarihi

24 Mart 2020

Yayımlandığı Sayı

Yıl 2020 Cilt: 8 Sayı: 1

DOI

https://doi.org/10.37696/nkmj.679069

IZ

https://izlik.org/JA33TZ25RG

Kaynak Göster

RIS / Bibtex

APA

Polat, E. (2020). Üç Boyutlu Hücre Kültürü Sistemlerine Güncel Yaklaşımlar. Namık Kemal Tıp Dergisi, 8(1), 84-92. https://doi.org/10.37696/nkmj.679069

AMA

1.Polat E. Üç Boyutlu Hücre Kültürü Sistemlerine Güncel Yaklaşımlar. NKMJ. 2020;8(1):84-92. doi:10.37696/nkmj.679069

Chicago

Polat, Elif. 2020. “Üç Boyutlu Hücre Kültürü Sistemlerine Güncel Yaklaşımlar”. Namık Kemal Tıp Dergisi 8 (1): 84-92. https://doi.org/10.37696/nkmj.679069.

EndNote

Polat E (01 Nisan 2020) Üç Boyutlu Hücre Kültürü Sistemlerine Güncel Yaklaşımlar. Namık Kemal Tıp Dergisi 8 1 84–92.

IEEE

[1]E. Polat, “Üç Boyutlu Hücre Kültürü Sistemlerine Güncel Yaklaşımlar”, NKMJ, c. 8, sy 1, ss. 84–92, Nis. 2020, doi: 10.37696/nkmj.679069.

ISNAD

Polat, Elif. “Üç Boyutlu Hücre Kültürü Sistemlerine Güncel Yaklaşımlar”. Namık Kemal Tıp Dergisi 8/1 (01 Nisan 2020): 84-92. https://doi.org/10.37696/nkmj.679069.

JAMA

1.Polat E. Üç Boyutlu Hücre Kültürü Sistemlerine Güncel Yaklaşımlar. NKMJ. 2020;8:84–92.

MLA

Polat, Elif. “Üç Boyutlu Hücre Kültürü Sistemlerine Güncel Yaklaşımlar”. Namık Kemal Tıp Dergisi, c. 8, sy 1, Nisan 2020, ss. 84-92, doi:10.37696/nkmj.679069.

Vancouver

1.Elif Polat. Üç Boyutlu Hücre Kültürü Sistemlerine Güncel Yaklaşımlar. NKMJ. 01 Nisan 2020;8(1):84-92. doi:10.37696/nkmj.679069