Extraction and Purification of Eriocitrin from Mentha piperita
Öz
Currently, the practice of using plants for treatment is a multidisciplinary field referred to as "phytotherapy." The presence of secondary metabolites in plants is one of the main reasons for their utilization in the pharmaceutical sector. Research assessing the scientific use of plants utilized in Traditional and Complementary Medicine (GETAT) inside modern medicine would enhance the healthcare system by delivering highly efficient pharmacological molecules with minimal adverse effects, potentially stimulating the country's economy. In this article, the levels of 16 specific phenolic compounds in Mentha piperita, Mentha dumoretum, Mentha spicata and Mentha villosa nervata were investigated and the purification of eriocitrine from Mentha piperita was attempted. Eriocitrin is a secondary metabolite that has been shown to have various benefits for human health. The process of purifying bioflavonoid combinations containing Eriocitrin from local natural sources is becoming increasingly important. The HPLC analysis revealed that the Butanol extract of Mentha piperita contained the highest concentrations of eriocitrin. Therefore, the purification process was carried out utilizing these extracts obtained from Mentha piperita. This research has yielded eriocitrin with a purity of 92%.
Anahtar Kelimeler
Destekleyen Kurum
Sağlık Bilimleri Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi
Proje Numarası
2022/047
Etik Beyan
Bu çalışma Sağlık Bilimleri Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi'nin 2022/47 Proje Numarası ile finansal destek almıştır.
Teşekkür
Yazarlar finansal desteği için Sağlık Bilimleri Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimine teşekkür ederler.
Kaynakça
- Akşit, H., Çelik, S. M., Şen, Ö., Erenler, R., Demirtaş, I., Telci, I., & Elmastaş, M. (2014). Complete isolation and characterization of polar portion of Mentha dumetorum water extract. Records of Natural Products, 8(3).
- Ángeles Ávila-Gálvez, M., Antonio Giménez-Bastida, J., González-Sarrías, A., & Espín, J. C. (2021). New Insights into the Metabolism of the Flavanones Eriocitrin and Hesperidin: A Comparative Human Pharmacokinetic Study. Antioxidants, 10(3).
- Areias, F. M., Valentão, P., Andrade, P. B., Ferreres, F., & Seabra, R. M. (2001). Phenolic fingerprint of peppermint leaves. Food Chemistry, 73(3). https://doi.org/10.1016/S0308-8146(00)00302-2
- Athanasiadis, V., Palaiogiannis, D., Grigorakis, S., Bozinou, E., Lalas, S. I., & Makris, D. P. (2023). Food-grade deep eutectic solvent extraction of antioxidant polyphenols from peppermint (Mentha × piperita L.): Screening, optimization and metabolite profile. Journal of Applied Research on Medicinal and Aromatic Plants, 33. https://doi.org/10.1016/j.jarmap.2022.100456
- Atoui, A. K., Mansouri, A., Boskou, G., & Kefalas, P. (2005). Tea and herbal infusions: Their antioxidant activity and phenolic profile. Food Chemistry, 89(1). https://doi.org/10.1016/j.foodchem.2004.01.075
- Demirtas, I., Erenler, R., Elmastas, M., & Goktasoglu, A. (2013). Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble fraction. Food Chemistry, 136(1), 34–40. https://doi.org/10.1016/j.foodchem.2012.07.086
- Dorman, H. J. D., Koşar, M., Kahlos, K., Holm, Y., & Hiltunen, R. (2003). Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varieties, and cultivars. Journal of Agricultural and Food Chemistry, 51(16). https://doi.org/10.1021/jf034108k
- Duband, F., Carnat, A. P., Carnat, A., Petitjean-Freytet, C., Clair, G., & Lamaison, J. L. (1992). COMPOSITION AROMATIQUE ET POLYPHENOLIQUE DE L’INFUSE DE MENTHE, MENTHA X PIPERITA L. Annales Pharmaceutiques Francaises, 50(3).
- Erenler, R., Yıldız, İ., Geçer, E. N., Yıldırım Kocaman, A., Alma, M. H., Demirtas, İ., Başar, Y., Hosaflıoğlu, İ., & Behçet, L. (2024). Phytochemical analyses of Ebenus haussknechtii flowers: Quantification of phenolics, antioxidants effect, and molecular docking studies. Bütünleyici ve Anadolu Tıbbı Dergisi, 5(2), 1–9. https://doi.org/10.53445/batd.1479874
- Fatih, B., Madani, K., Chibane, M., & Duez, P. (2017). Chemical Composition and Biological Activities of Mentha Species. In Aromatic and Medicinal Plants - Back to Nature. InTech. https://doi.org/10.5772/67291
