Ekmeklik Buğdayda Geliştirilen Rekombinant Kendilenmiş Hat Populasyonunda Kalite Özellikleri İçin Fenotipik ve Genotipik Değişim

Öz

Rekombinant kendilenmiş hat populasyonları tarımsal araştırmalarda kullanılan önemli haritalama populasyonlarıdır. Çalışmada, Tosunbey ve Tahirova-2000 çeşitleri melezlenerek 420 ve 362 adet hat bulunan resiprokal rekombinant kendilenmiş hat populasyonu geliştirilmiştir. Populasyonların geliştirilmesinde tek tohum soy yöntemi kullanılmıştır. F7 generasyondaki homozigot hatlar, Samsun lokasyonunda Agumented deneme desenine göre 6 m2 alanındaki parsellerde yetiştirilmiştir. Hatların Yüksek ve Düşük Molekül Ağırlıklı Glutenin Alt Üniteleri (YMA-GA ve DMA-GA) ile çavdar translokasyonu varlığı SDS-PAGE yöntemiyle belirlenmiştir. Tosunbey çeşidi YMA-GA için 1, 17+18, 5+10 alt ünitelerini ve DMA-GA için GluA3b, GluB3b ve GluD3b allellerini taşırken, Tahirova-2000 çeşidi YMA-GA için 2*, 7+9, 5+10 alt ünitelerini ve DMA-GA için GluA3e, GluB3j ve GluD3b allellerini ve 1BL.1RS çavdar translokasyonu taşımaktadır. Denemede tane verimi, bitki boyu, başaklanma gün süresi gibi agronomik özellikler yanında protein içeriği, SDS sedimentasyon değeri, hektolitre ağırlığı, bin tane ağırlığı, kül oranı ve L renk değeri saptanmıştır. Tosunbey × Tahirova-2000ve Tahirova-2000 × Tosunbey populasyonlarında denemelerin ortalama tane verimi sırasıyla 399.5 kgda-1 ve 328.9 kgda-1’dır. Populasyonlarda bitki boyu ve başaklanma süresi için önemli seviyede transgresif açılım gözlenmiştir. Üçyüzyirmidört hattın çavdar translokasyonu (1BL.1RS) taşıdığı ve çavdar translokasyonunun protein kalitesine etkisinin olumsuz olduğu saptanmıştır. GluA3b alleli taşıyan hatların SDS sedimentasyon değeri, GluA3e alleli taşıyanlara kıyasla daha yüksektir. Araştırma sonuçları populasyonların ekmeklik buğdayda kalite ve kuraklık çalışmalarında kullanılabilecek elit materyale sahip olduğunu göstermiştir.

Anahtar Kelimeler

• ekmeklik buğday
• RILs
• YMA ve DMA Glutenin
• çavdar translokasyonu

Phenotypic and Genotypic Variation For Quality Traits In The Recombinant Inbred Line Population Developed in Bread Wheat

Öz

Recombinant inbred line (RILs) populations are substantial mapping populations used in agricultural research.In the study, a reciprocal recombinant inbred line population with 420 and 362 lines was developed by crossing Tosunbey and Tahirova-2000 varieties. The single-seed descent method was used to create the mapping populations. RILs in the F7 generation were grown in plots in a 6 m2 area according to the Augmented design in Samsun location. The presence of rye translocation and High and Low Molecular Weight Gluten Subunits (HMW-GS and LMW-GS) of the lines was determined by the SDS-PAGE method. Tosunbey variety has 1, 17+18, 5+10 subunits for YMA-GA and GluA3b, GluB3b, GluD3b alleles for LMW-GS, while Tahirova-2000 variety has 2*, 7+9, 5+10 subunits for HMW-GS and GluA3e, GluB3j, GluD3b alleles for DMA-GA and 1BL.1RS rye translocation. The data of grain yield, plant height, and heading time were recorded as agronomic traits, while protein content, SDS sedimentation value, hectoliter weight, thousand-grain weight, ash ratio, and L color value as the quality traits. The average grain yield of Tosunbey × Tahirova-2000and Tahirova-2000 × Tosunbey populations is 399.5 kg/da and 328.9 kg/da, respectively. Significant transgressive segregation was observed for plant height and heading time in the mapping populations. Three hundred twenty-four lines have rye translocation (1BL.1RS), and the effect of rye translocation on protein quality was negative. The SDS sedimentation value of the lines with the GluA3b allele is higher than those with the GluA3e allele. These results indicated that the populations have the elite lines for bread wheat quality and drought studies.

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