Saf Gerze tavuğu ve Sinop ili köy tavuğu popülasyonlarının sekiz polimorfik mikrosatellit lokusu bakımından karşılaştırılması

Yıl 2019, Cilt: 34 Sayı: 2, 164 - 171, 25.06.2019

Levent Mercan , Fatih Bilgi , Merve Budak

https://doi.org/10.7161/omuanajas.480586

Cited By: 1

Öz

Araştırma, Sinop ili Gerze ilçesinde
bulunan koruma altındaki saf Gerze tavuğu popülasyonu ve aynı ilde
yetiştiriciliği yapılan köy tavuğu popülasyonları arasındaki genetik ilişkinin
belirlenmesi amacıyla yürütülmüştür. Çalışmada yüksek düzeyde polimorfizm
gösteren 8 adet mikrosatellit lokusu kullanılmıştır. Çalışmanın hayvan
materyalini, Sinop ili Ayancık, Boyabat ve Gerze ilçelerinde bulunan 3 köy
tavuğu popülasyonuna ait 60 örnek ve Gerze ilçesinde koruma altında tutulan 20
adet saf Gerze tavuğu oluşturmuştur. Sinop Gerze Köy, Sinop Gerze (Hacıkadı),
Sinop Ayancık Köy ve Sinop Boyabat Köy popülasyonlarında beklenen
heterozigotluk (H_e) değerleri sırasıyla 0.820, 0.755, 0.795 ve 0.741
olarak hesaplanmıştır. Popülasyon ayrımı yapılmaksızın hesaplanan akrabalı
yetiştirme katsayısı (F_IS) değerleri LEI0166, MCW0034 ve MCW0069
lokuslarında -0.131, -0.195, -0.137 ve ADL0278, LEI0234, MCW0080, MCW0104,
MCW0330 lokuslarında ise sırasıyla 0.178, 0.134, 0.766, 0.508 ve 0.433 olarak
bulunmuştur. Temel koordinatlar ve Structure analizi sonuçları saf Gerze
(Hacıkadı) popülasyonunun diğer köy popülasyonlarından genetik olarak farklı
olduğunu ortaya koymuştur. Araştırmada, halk elinde yetiştiriciliği yapılan
Gerze tavuklarının saf olmadığı, bu ırkın korunması için bölgede saf olarak
yetiştirilmesinin gerekli olduğu sonucuna varılmıştır.

Anahtar Kelimeler

Genetik çeşitlilik, Köy tavuğu, Mikrosatellitler, Sinop

Comparison of Gerze and village chicken populations in Sinop by eight polymorphic microsatellite loci

Öz

In this study, we aimed to identify the genetic
relation between indigenous Gerze chicken population which is conserved in
Gerze district of Sinop province and village chicken populations which are
raised in Sinop. We used 8 highly polymorphic microsatellite loci for
examinations. Animal materials of this study are total of 60 samples from 3
village chicken populations raised in Ayancık, Boyabat and Gerze districts, and
20 samples from Gerze chicken from the conserved population in Gerze district
of Sinop province. Expected heterozygosity (H_e) values in Sinop
Gerze Village, Sinop Gerze (Hacıkadı), Sinop Ayancık Village and Sinop Boyabat
Village populations were calculated as 0.820, 0.755, 0.795 and 0.741,
respectively. F_IS values were predicted as -0.131, -0.195, -0.137 in
LEI0166, MCW0034 ve MCW0069 loci and 0.178, 0.134, 0.766, 0.508, 0.433 in
ADL0278, LEI0234, MCW0080, MCW0104, MCW0330 loci respectively. Principal
coordinate and Structure analyses’ results showed that Gerze (Hacıkadın)
population is genetically different from the other village populations. In the
study, it was concluded that the chickens in the Gerze villages are not pure
breed. Thus, pure breed raising is necessary to conserve this breed in the
region.

Anahtar Kelimeler

Genetic diversity, Village chicken, Microsatellites, Sinop

Kaynakça

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