Therapeutic potential of morus nigra on 5-fluorouracil-induced gastrointestinal mucositis in rats

Öz

To investigate the therapeutic potential of Morus Nigra on the mucositis of digestive system induced via 5-fluorouracil in rats. A total of 26 rats were divided into 4 groups as control (C), mucositis (M), Morus nigra extract-1(MN-1) and Morus nigra extract-2 (MN-2). Mucositis was induced by intraperitoneal injection of 60 mg/kg 5-fluorouracil (5-FU). The antioxidant enzyme levels in blood samples were analysed by ELISA. Histological analysis and immunohistochemical staining for NF-kB and TNF-α were done. Increased oxidative markers were found in mucositis group and increased antioxidative status was found in MN-1 and MN-2 groups. All histochemical analyses suggested that 5-FU causes intestinal degeneration; the MN-1 extract reduced this degeneration but did not completely treat it. The therapeutic potential of MN-2 extract was lower than the MN-1. Results of the present study suggest that, though partly, MN-1 extract in particular may have biochemically and histologically positive effects against experimental mucositis induced by 5-FU in rats.

Anahtar Kelimeler

• Mucositis; Rat; Morus Nigra; 5-Fluorouracil

Morus Nigra'nın sıçanlarda 5-florourasil ile oluşturulmuş gastrointestinal mukozite karşı olası tedavi ediciliği

Öz

Bu çalışmanın amacı sıçan gastrointestinal mukozasında 5-florourasil ile oluşturulmuş mukozit üzerine Morus nigra ekstresinin olası tedavi edici etkisini araştırmaktır.Toplam 26 adet sıçan eşit olarak kontrol, mukozit, mukozit+morus nigra ekstre 1 (MN-1) ve mukozit + morus nigra ekstre 2 (MN-2) olarak bölünmüştür. Mukozit sıçanlarda 60 mg/kg 5-fluorourasilin (5-FU) intraperitoneal olarak enjeksiyonuyla oluşturulmuştur. ELISA ile kandaki oksidan ve antioksidan seviyeleri ölçüldü. Histokimyasal ve immunohistokimyasal boyamalar (NF-kB ve TNF-α antikorları için) yapıldı.  Mukozit grubunda artmış oksidatif stres belirteçleri görülürken Morus nigra ekstresi verilen gruplarda antioksidan belirteçlerin arttığı saptandı. Histokimyasal olarak 5-FU'nun mukozit oluşturduğu ve MN-1 ekstresinin tam olarak olmasa da tedavi edici olduğu gösterildi. MN-1 ekstresi MN-2 ekstresine göre daha iyi bir tedavi edici ajan olduğu belirlendi. Sonuç olarak bu çalışmada 5-FU ile oluşturulmuş mukozite karşı MN-1 ekstresinin kısmi olarak tedavi edici olduğu biyokimyasal ve histopatolojik olarak gösterildi.

Anahtar Kelimeler

• Mukozit,Sıçan,Morus nigra,5-florourasil

Kaynakça

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