Preparation of Antimicrobial Agent Loaded Microcapsules For Medical Textiles

Abstract

The aim of this work is to develop microcapsules loaded with antimicrobial agent to apply medical textile products. For this purpose St. John's Wort oil (Hypericum perforatum) with antimicrobial activity was encapsulated by complex coacervation method using gelatin (GE) and gum arabic (GA) as wall material. The effect of various processing parameters, including the amount of oil, amount of surfactant and stirring rate at hardening stage on the encapsulation yield, particle size distribution and capsule loading was investigated. In general, at high oil content the encapsulation yield, capsule size and oil loading increased. As expected an increase in the amount of surfactant decreased the capsule size. In this case, the loading was low, as well. High stirring rate increased the encapsulation yield and capsule siz.

Keywords

• Microencapsulation, Complex coacervation, St. John's Wort oil, Gelatin, Gum arabic.

Medikal Tekstiller İçin Antimikrobiyel Ajan İçeren Mikrokapsüllerin Hazırlanması

Öz

Bu çalışmanın amacı, medikal amaçlı tekstil ürünlerine uygulanabilecek antimikrobiyal ajan içeren mikrokapsüllerin geliştirmektir. Bu amaçla kabuk maddesi olarak jelatin ve arap zamkı kullanılarak, antimikrobiyal özelliği olan sarı kantaron yağı kompleks koaservasyon yöntemi ile kapsüllenmiştir. Yağ miktarı, surfaktan miktarı ve kapsüllerin sertleştirilmesi aşamasındaki karıştırma hızının kapsüllenme verimi, parçacık boyut dağılımı ve mikrokapsüllerin içerdiği yağ miktarına etkileri araştırılmıştır. Genel olarak yüksek yağ miktarında kapsüllenme verimi, kapsül boyutu ve kapsüllerin yağ içeriği artmıştır. Beklenildiği gibi surfaktan miktarındaki artış kapsül boyutunu küçültmüştür. Bu durumda kapsüllerin içerdiği yağ miktarı da düşük olmuştur. Yüksek karıştırma hızı kapsüllenme verimliliğini ve kapsül büyüklüğünü artırmıştır.

Anahtar Kelimeler

• Mikrokapsülasyon, Kompleks koaservasyon, Sarı kantaron yağı, Jelatin, Arap zamkı.

Kaynakça

1. Augustin, M. A., Hemar, Y., “Nano- and Micro-Structured Assemblies for Encapsulation of Food Ingredients”, Chem. Soc. Rev., 38: 902-912, 2009.
2. Benita, S., Microencapsulation: Methods and Industrial Applications, Marcel Dekker, Inc.,New York, USA, 1996.
3. Liu, S., Low, N. H., Nickerson, M. T., “Entrapment of Flaxseed Oil Within Gelatin – Gum Arabic Capsules”, Journal of the American Oil Chem. Soc., 87 (7): 809-815, 2010.
4. Yeo, Y., Bellas, E., Firestone, W., Langer, R., Kohane, D. S., “Complex Coacervates for Thermally Sensitive Controlled Release of Flavor Compounds”, J. Agric. Food Chem., 53: 7518-7525, 2005.
5. Aguilera, J. M., Lillford, P. J., 2, Food Materials Science: Princiles and Practice, Food Engineering Series, Springer Science+Busines Media, LLC, New York, USA, 2008.
6. Huang, Y-I., Cheng, Y-H., Yu, C-C., Tsai, T-R., Cham, T-M., “Microencapsulation of Extract Containing Shikonin Using Gelatin – Acacia Coacervation Method: A Formaldehyde- free Approach”, Colloids and Surfaces B: Biointerfaces, 58: 290-297, 2007.
7. Vahapzadeh, F., Zivdar, M., Najafi, A., “Microencapsulation of Orange Oil By Complex Coacervation and Its Release Behavior”, IJE Transactions B: Applications, 17 (4): 333-342, 2004.
8. Green, B. K. , Oil-Containing Microscopic Capsules and Method of Making Them, U. S. Patent, US 2,800,457, U. S. A., 1957.

9. Bansode, S. S., Banarjee, S. K., Gaikwad, D. D., Jadhav, S. L., Thorat, R. International Journal of Pharmaceutical Sciences Review and Research, 1 (2): 38-43, 2010. A Review”,
10. Cosco, S., Polymer Based Microparticles for Advanced Composite Materials Applications,Ph. D. Thesis, University of Naples ‘Federico II’ Department of Materials and Productions Engineering, Italy, 2006.
11. Harrington, J. C., Morris, E. R., “Conformational Ordering and Gelation in Mixtures with Soluble Polysaccharides”, Food Hydrocolloids, 23: 327-336, 2009.
12. Kong, X. Z., Gu, X., Zhu, X., Zhang, Z., “Spreadable Dispersion of Insect Sex Pheromone Capsules, Preparation Via Complex Coacervation And Release Control of The Encapsulated Pheromone Component Molecule”, Biomed Microdevices, 11: 275-285, 2009.
13. Dong, Z. J., Touré, A., Jıa, C. S., Zhang, X. M., Xu, S. Y., “Effect of Processing Parameters on the Formation of Spherical Multinuclear Microcapsules Encapsulating Peppermint Oil By Coacervation”, Journal of Microencapsulation, 24 (7): 634-646, 2007.
14. Barbafallo, C., Chisari, G.,“Antimicrobial activity of three Hypericum species”, Fitoterapia, 58: 175-177, 1987.
15. Avato, P., Raffo, F., Guglielmi, G., Vitali C., Rosato A., “Extracts From St. John’s Wort and Their Antimicrobial Activity”, Phytotherapy Research, 18: 230-232, 2004.
16. Övez, B., Yuksel, M., “Parfümlerin Çapraz Bağlı Mikrokapsüllerden Yavaş Salgılanmaları”, Ekoloji Issue, 10 (43): 26-29, 2002.
17. Xing, F., Cheng, G., Yang, B., Ma, L., “Microencapsulation of Capsaicin by the Complex Coacervation of Gelatin, Acacia and Tannins”, Journal of Applied Polymer Science, 91: 2669-2675, 2004.