Echinacoside decreases cell proliferation and inhibits cell invasion in PC3 androgen-independent prostate cancer cells

Abstract

Purpose: The aim of this study was to determine the effects of Echinacoside on cell proliferation, invasion and mRNA expression changes of invasion-related genes in PC3 androgen-independent prostate cancer cells.
Material and methods: The effect of Echinacoside on cell proliferation in PC3 cells was determined by XTT method. Anti-invasive efficacy was achieved using the transwell chamber. Total RNA isolation was performed by Trizol and cDNA was subsequently synthesized. mRNA expression changes in MMP2, MMP9, TIMP1, TIMP2 and TIMP3 were also performed in RT-PCR with SYBER Green.
Results: In this study, the IC50 dose of Echinacoside in PC3 cells was determined as 55.21 μM at 48h. It was determined that echinacoside inhibited cell invasion in PC3 cells and reduced the invasion by 66% in the dose group. In addition, it was found statistically significant that Echinacoside increased TIMP1 mRNA expression 1.96 times, TIMP2 mRNA expression 2.60 times, while decreasing MMP2 expression 3.82 times and MMP9 mRNA expression 1.54 times in IC50 dose group according to control.
Conclusion: It was revealed that echinacoside has an anti-proliferative effect on PC3 prostate cancer cells. It has also been shown that invasion-related genes can suppress invasion by regulating expression changes. With this study, preliminary data were presented in terms of detailed molecular biological studies to be carried out on echinacoside and its effect on prostate cancer.

Keywords

• Echinacoside
• PC3 cells
• androgen-independent prostate cancer

Ekinakozit PC3 androjen bağımsız prostat kanseri hücrelerinde hücre proliferasyonunu azaltır ve hücre invazyonunu inhibe eder

Öz

Amaç: Bu çalışmanın amacı Echinacoside'in PC3 androjen bağımsız prostat kanseri hücrelerinde hücre proliferasyonuna, invazyonuna ve invazyon ilişkili genlerin mRNA ekspresyon değişimleri üzerine etkilerini belirlemektir.
Gereç ve yöntem: Ekinakozitin PC3 hücrelerinde hücre proliferasyonuna olan etkisi XTT yöntemiyle belirlenmiştir. Anti-invaziv etkinliği transwel chamber kullanılarak gerçekleştirilmiştir. Total RNA izolasyonu Trizol aracılığıyla gerçekleştirilmiş ve takiben cDNA sentezlenmiştir. MMP2, MMP9, TIMP1, TIMP2 ve TIMP3'ün mRNA ekspresyon değişimleri SYBER Green ile RT-PCR da gerçekleştirilmiştir.
Bulgular: Bu çalışmada Ekinakozitin PC3 hücrelerinde IC50 dozu 48. saate 55,21 μM olarak tespit edilmiştir. Ekinakozitin PC3 hücrelerinde hücre invazyonunu inhibe ettiği doz grubunda %66 oranında invazyonu azalttığı belirlenmiştir. Ayrıca ekinakozitin kontrole göre IC50 doz grubunda, TIMP1 mRNA ekspresyonunu 1,96 kat, TIMP2 mRNA ekspresyonunu 2,60 kat arttırken MMP2 ekspresyonunu 3,82 kat, MMP9 mRNA ekspresyonunu 1,54 kat azaltması istatistiksel olarak anlamlı bulunmuştur.
Sonuç: Ekinakozitin PC3 prostat kanseri hücreleri üzerinde antiproliferatif etki gösterdiği ortaya koyulmuştur. Ayrıca invazyon ilişkili genlerin ekspresyon değişimlerini regüle ederek invazyonu da baskılayabileceği gösterilmiştir. Bu çalışma ile ekinakozit ve prostat kanseri üzerindeki etkisi ile ilgili olarak bundan sonraki yapılacak olan detaylı moleküler biyolojik çalışmalar açısından ön veriler ortaya koyulmuştur.

Anahtar Kelimeler

• Ekinakozit
• PC3 hücreleri
• androjen bağımsız prostat kanseri

Kaynakça

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