Investigation of the protective effect of rosmarinic acid in rats given high dose gentamicin

Abstract

Purpose: Gentamicin is an antibiotic, used to treat infections caused by microorganisms. Endogenous antioxidants are strengthened by using antioxidant protectors against the toxic effects caused by drugs. Rosmarinic acid has antioxidative, anti-inflammatory, antiapoptotic, and antitumoral effects. In our study, we aimed to investigate the protective effect, immunohistochemical changes, oxidative markers, histopathological changes and inflammation related Ifi44 expression of rosmarinic acid against gentamicin induced nephrotoxicity in rats. Materials and methods: In our study, 32 rats randomly divided into four groups. These groups are consisted of control group, gentamicin group received gentamicin 100 mg/kg/day, gentamicin + rosmarinic group acid received gentamicin 100 mg/kg/day and rosmarinic acid 50 mg/kg/day, and rosmarinic acid group received rosmarinic acid 50 mg/kg/day. At the end of the study, histopathological, immunohistochemical, and biochemical changes in kidney tissues evaluated. Results: Rosmarinic acid reduced creatine, urea, blood urea nitrogen, and total oxidative stress in blood serum. The toxic effect of gentamicin caused severe histopathological changes in the kidneys. A slight decrease in histopathological changes observed in the gentamicin + rosmarinic acid group. Antiproliferative Ifi44 expression was higher in the gentamicin group and gentamicin + rosmarinic acid group. Conclusion: As a result of the application of rosmarinic acid, a decrease in oxygen radicals and an increase in antioxidant levels observed. When used in combination with gentamicin and rosmarinic acid, the protective effect of rosmarinic acid was partially observed, but it could not provide full protection.

Keywords

• Gentamicin
• rosmarinic acid
• Ifi44
• rat

Yüksek doz gentamisin verilen sıçanlarda rosmarinik asidin koruyucu etkisinin araştırılması

Öz

Amaç: Gentamisin, mikroorganizmaların neden olduğu enfeksiyonları tedavi etmek için kullanılan bir antibiyotiktir. İlaçların neden olduğu toksik etkilere karşı antioksidan koruyucular kullanılarak endojen antioksidanlar güçlendirilir. Rosmarinik asit antioksidan, antienflamatuar, antiapoptotik ve antitümör etkilere sahiptir. Çalışmamızda rosmarinik asidin sıçanlarda gentamisin ile indüklenen nefrotoksisiteye karşı koruyucu etkisini, immünohistokimyasal değişiklikleri, oksidatif belirteçleri, histopatolojik değişiklikleri ve enflamasyona bağlı Ifi44 ekspresyonunu araştırmayı amaçladık. Gereç ve yöntem: Çalışmamızda 32 rat rastgele 4 gruba ayrıldı. Bu gruplar kontrol grubu, gentamisin grubu 100 mg/kg/gün gentamisin verildi, gentamisin + rosmarinik asit grubu gentamisin 100 mg/kg/gün ve rosmarinik asit 50 mg/kg/gün verildi, rosmarinik asit grubu rosmarinik asit 50 mg/kg/gün verildi. Çalışma sonunda böbrek dokularındaki histopatolojik, immünohistokimyasal ve biyokimyasal değişiklikler değerlendirildi. Bulgular: Rosmarinik asit, kan serumunda kreatin, üre, kan üre nitrojeni ve toplam oksidatif stresi azaltmıştır. Gentamisinin toksik etkisi böbreklerde ciddi histopatolojik değişikliklere neden olmuştur. Gentamisin + rosmarinik asit grubunda histopatolojik değişikliklerde hafif bir azalma gözlendi. Antiproliferatif Ifi44 ekspresyonu gentamisin grubunda ve gentamisin + rosmarinik asit grubunda daha yüksekti. Sonuç: Rosmarinik asit uygulaması sonucunda oksijen radikallerinde azalma ve antioksidan düzeylerinde artış gözlendi. Gentamisin ve rosmarinik asit birlikte kullanıldığında rosmarinik asidin koruyucu etkisi kısmen gözlenmiştir ancak tam koruma sağlayamamıştır.

Anahtar Kelimeler

• Gentamisin
• rosmarinik asit
• Ifi44
• sıçan

Kaynakça

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