Evaluation of serum matrix metalloproteinase 2-7-9, tissue matrix metalloproteinase inhibitor 1, vascular endothelial growth factor, interleukin 6 and interleukin 8 levels in breast cancinoma

Öz

Purpose: The raising incidence of breast cancer and development of treatment modalities cause prolongation of survival and new markers are needed for follow up. Serum matrix metalloproteinase 2, 7, 9 (MMP2, MMP7, MMP9), tissue matrix metalloproteinase inhibitor 1 (TIMP1), interleukin 6, interleukin 8 and vasculer endotelial growth factor (VEGF) take different parts on cancerogenesis. The aim of study is to evaluate the relationship between these markers and clinical stage, histopathologic features and laboratuary parameters.

Materials and methods: Seventyeight pathologically proven, chemotherapy naive breast cancer patients were enrolled. The analyses were carried out using SPSS v15 and p<0.05 was accepted as significant. Descriptive studies were analyzed using chi-square test and Mann-Whitney U-test depending on group numbers.

Results: The median age of patients were 52±11.6. Patients were divided to non-metastatic and metastatic pattern. VEGF values were 3.4 fold higher in metastatic group. Raising of CA15-3 levels were statistically correlated with MMP9 and IL6. The elevation of LDH were associated with raised VEGF and TIMP1. CRP elevation was correlated with increased IL8, IL6 and MMP9. Raising of VEGF was associated with progression. VEGF levels of patients lost in follow up were higher although there was no statistically significance. MMP7 levels were lower in hormon positive group than negative group. MMP2 levels were higher in HER2 (3+) group than HER2 (0/1+/2+) patient group.  TIMP1 levels were lower in patients whom ki 67 ratios were above 40% than below 40%.

Conclusion: This is a preliminary study with low patient number and short follow up time.

Anahtar Kelimeler

• Breast cancer
• VEGF
• IL6
• IL8

Meme kanserinde serum matriks metalloproteinaz 2-7-9, doku matriks metalloproteinaz inhibitörü 1, vasküler endotelial büyüme faktörü, interlökin 6 ve interlökin 8 düzeylerinin değerlendirilmesi

Öz

Amaç: Meme kanseri insidansının artması ve gelişen tedaviler ile sağkalımın uzaması izlemde yeni belirteçlere gereksinim ortaya çıkarmaktadır. Serum matriks metalloproteinaz 2, 7, 9 (MMP2, MMP7, MMP9), doku matriks metalloproteinaz inhibitörü1 (TIMP1), interlökin 6, interlökin 8 ve vasküler endoteliyal büyüme faktörü (VEGF) kanserogenezin farklı basamaklarında rol almaktadırlar. Çalışmamızın amacı meme kanseri hastalarında bu parametrelerin klinik evre, histopatolojik özellikler ve laboratuar değerleri üzerine etkilerini değerlendirmektir.

Gereç ve Yöntem: Meme kanseri patolojik tanısı konmuş kemoterapi naif yetmiş sekiz kadın hasta çalışmaya alındı. İstatistik, Statistical Package for Social Sciences version15 ile hesaplandı. Sonuçlar %95 güven aralığında değerlendirildi. p<0,05 olması istatistiksel olarak anlamlı kabul edildi. Hasta gruplarını karşılaştırmalarında ki-kare ve Mann Whitney-U testi uygulandı.

Bulgular: Hastaların medyan yaşı 52±11,6 bulundu. Hastalar “non-metastatik” ve “metastatik” şeklinde iki gruba ayrıldı. Metastatik grupta serum VEGF değeri 3,4 kat yüksek saptandı. Serum CA15-3 seviyesinde yükselme, artmış MMP9 ve IL6 seviyeleri ile anlamlı ilişkiliydi. Serum LDH seviyesi artmış hastalarda serum VEGF ve TIMP1 seviyeleri anlamlı arttı. Serum CRP artışı IL8, IL6 ve MMP9 artışı ile ilişkili bulundu. Serum VEGF seviyeleri progrese olgularda yüksekti. İzlemde kaybedilen hastaların serum VEGF seviyesi daha yüksek olmakla beraber istatistiksel anlamlı değildi. Hormon pozitif grupta negatif gruba göre serum MMP7 seviyeleri anlamlı düşük saptandı. HER2 (3+) hasta grubunda serum MMP2 seviyeleri HER2 (0/1+/2+) hasta grubuna göre daha yüksekti. Serum TIMP1 seviyesi ki 67 oranı %40 ve üzeri hastalarda, oran %40 altında olan hastalara göre anlamlı düşük bulundu.

Sonuç: Çalışmamız kısıtlı hasta sayısı ile yapılan kısa takip süresine sahip bir ön çalışma özelliğindedir.

Anahtar Kelimeler

• Meme kanseri
• VEGF
• IL6
• IL8

Kaynakça

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