The ameliorative effects of kaempferol, curcumin and kaempferol-curcumin combination on experimental acute pancreatitis

Öz

Aim: Acute pancreatitis is a medical emergency disorder which manifests itself with sudden onset abdominal pain and may progress systemic inflammatory response syndrome. Novel treatment modalities are required for this disorder which does not have standard treatment regime. We aimed to evaluate the effects of kaempferol, curcumin and kaempferol-curcumin combination on experimental acute pancreatitis.

Material and method: Wistar albino adult rats were divided into 6 groups. Group I: saline was given by intraperitoneal(ip). Group II: L-arginine was given by ip at a dose of 250 mg per 100 gr. Group III(DMSO): Dimethyl sulphoxide (DMSO) was given by ip at a dose of 1 ml/100 gr. Group IV(L-arginine+curcumin): L-arginine and curcumin at a dose of 50 mg/kg was given by ip, Group V(L-arginine+ Kaempferol): L-arginine and kaempferol at a dose of 21 mg/kg was given by ip. Group VI(L-arginine+curcumin + Kaempferol): L-arginine, curcumin at a dose of 50 mg/kg and kaempferol at a dose of  21 mg/kg was given by ip. Blood and pancreas sample of the rats were obtained.

Results: Malondialdehyde and IL-6 levels were not different between groups. Superoxide dismutase(SOD) levels were higher than other groups but it was not statistically significant. Edema score on pancreas tissue were lower than group II in group VI. Inflammatıon score were lower than group II in groups V and VI. NF(nuclear factor)- κB staining score were not different between groups.

Conclusion: Kaempferol and kaempferol-curcumin combination have protective effects on L-arginine induced experimental acute pancreatitis by diminishing inflammation and necrosis.

Anahtar Kelimeler

• arginine,curcumin,inflammation

Kaempferol, kurkumin ve kaempferol-kurkumin kombinasyonunun deneysel akut pankreatitteki etkileri

Öz

Amaç: Akut Pankreatit ani başlayan karın ağrısı ile kendini gösterip sistemik inflamatuar yanıta kadar ilerleyebilen acil bir klinik durumdur. Standart bir tedavisi olmayan bu hastalıkta güncel tedavi yaklaşımlarına gereksinim vardır. Deneysel akut pankreatitte kaempferol, kurkumin ve kaempferol-kurkumin kombinasyonunun etkilerini göstermeyi amaçladık.

Gereç ve Yöntem: Wistar albino erişkin ratlar altı gruba ayrıldı. Grup I: Sıçanlara fizyolojik su intraperitoneal (i.p.) olarak verildi. Grup II: L-arjinin i.p. olarak 250 mg/100 gr, Grup III(DMSO): İ.p. olarak 1 ml/100 gr Dimetil sülfoksit (DMSO), Grup IV(L-Arjinin + Kurkumin): i.p.  L-Arginin ve kurkumin 50 mg/kg İP, Grup V(L-Arjinin + Kaempferol): İ.p.   L-Arginin ve kaempferol 21 mg/kg İP, Grup VI(L-Arjinin + Kurkumin + Kaempferol): İ.p.  L-Arginin , kurkumin 50 mg/kg ve kaempferol 21 mg/kg i.p. uygulandı. Deney bitiminde kan ve pankreas örnekleri alındı. Histolojik, biyokimyasal ve immunohistokimyasal incelemeler yapıldı.

Bulgular: Malondialdehid, TNF-α ve IL-6 düzeyleri gruplar arasında farklı değildi. Superoksit dismutaz(SOD)  düzeyleri grup VI’da diğer gruplara göre yüksekti ancak istatistiksel olarak anlamlı değildi. Ödem skoru grup VI’da grup II’ye göre daha düşük idi. Grup V ve grup VI’da inflamasyon skoru grup II’ den daha az idi. Nekroz skoru grup IV, grup V ve grup VI’da grup II’den daha az idi. Grup II’deki NF(nükleer faktör)-κB boyanma skoru tedavi gruplarından farklı değildi.

Sonuç: L-arjinin ile oluşturulan deneysel akut pankreatitde kaempferolün ve kaempferol-kurkumin kombinasyonunun özellikle histopatolojik olarak inflamasyonu ve nekrozu azalttığı bu sayede koruyucu etkileri olduğu görülmüştür.

Anahtar Kelimeler

• arjinin,kurkumin,inflamasyon

Kaynakça

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