Yıl 2019, Cilt 12 , Sayı 3, Sayfalar 415 - 421 2019-09-30

Increased DNA damage with comet assay in patients with pulmonary hypertension
Pulmoner hipertansiyonlu hastalarda comet analizi ile değerlendirilen artmış DNA hasarı

Emine Kılıç-Toprak [1] , Yalın Tolga Yaylalı [2] , Yasin Özdemir [3] , Vural Küçükatay [4] , Hande Şenol [5] , Melek Bor-Küçükatay [6]


Purpose: In pulmonary hypertension (PH), pathologic lesions are characterized by oxidative stress and inflammatory processes. The purpose of our study was to investigate the potential contribution of DNA damage in PH.

 

Materials and methods: The study comprised 28 treatment-naive PH patients (59.93±11.19 years) and 28 age- and sex-matched (59.86±11.92 years) healthy controls. All participants underwent a right-heart catheterization to confirm the diagnosis and to asses hemodynamics. Venous blood was obtained from participants. DNA damage were evaluated using the comet assay which is based on single cell gel electrophoresis and fluorescent microscopy and detects DNA damage prior to repair. Continuous data were reported as mean ± standard deviation (SD). Shapiro–Wilk tests were used for testing normality. Mann Whitney U test was used for independent groups. For categorical variables, Chi-square test was used. SPSS, 24.0 was used for statistical analyses and p value less than 0.05 was considered statistically significant.

 

Results: The average pulmonary vascular resistance was 5.64±2.99 WU, mean right atrial pressure was 9.70±5.38 mmHg, cardiac index was 3.12±1.18 l/min/m2, mixed venous O2 saturation was 64.77±13.33 %. DNA damage parameters such as tail length (24.02±11.34 vs. 16.88±3.55 μm, p=0.0001), tail moment (1.93±2.36 vs. 0.87±1.03 μm, p=0.013), tail migration (10.3±12.24 vs. 3.62±2.75, p=0.03) were significantly higher in

patients with PH.

 

Conslusion: These results suggest that patients with PH could have increased DNA damage before DNA repair mechanisms are at play. DNA damage could be contributing to the pathophysiology and may represent a novel pharmacological target in PH.

Amaç: Pulmoner hipertansiyonda (PH) görülen patolojik lezyonlar, oksidatif stres ve inflamatuar süreçlerle karakterizedir. Çalışmamızın amacı, PH hastalarında DNA hasarının potansiyel katkısını araştırmaktır.

Gereç ve Yöntem: Çalışmamıza 28 tedavi almamış PH hastası (59.93 ± 11.19 yıl) ve 28 yaş-cinsiyet uyumlu (59.86 ± 11.92 yıl) sağlıklı kontrol dahil edilmiştir. Tüm hastalara tanıyı doğrulamak ve hemodinamiyi değerlendirmek için sağ kalp kateterizasyonu uygulanmıştır. Bireylerden alınan venöz kan örneklerinde, DNA hasarı belirlenmesinde tek hücre jel elektroforezi ve flüoresan mikroskopisine dayanan, onarımdan önce DNA hasarını tespit eden kuyruklu yıldız (Comet) analizi kullanılmıştır. Sürekli veriler ortalama ± standart sapma (SD) olarak verilmiş, normallik testi için Shapiro-Wilk testleri kullanılmıştır. Bağımsız gruplar için Mann Whitney U testi, kategorik değişkenler için ki-kare testi kullanılmıştır. İstatistiksel analizlerde SPSS, 24.0 programı kullanılmış ve p≤0.05 değerleri istatistiksel olarak anlamlı kabul edilmiştir.

Bulgular: Ortalama pulmoner vasküler direnç 5.64 ± 2.99 Wood Unitesi, ortalama sağ atriyal basınç 9.70 ± 5.38 mmHg, kardiyak indeks 3.12 ± 1.18 l / dak / m2, mixed venöz O2 saturasyonu % 64.77 ± 13.33 olarak saptanmıştır. DNA hasar parametrelerinden, kuyruk uzunluğu (24.02 ± 11.34 ve 16.88 ± 3.55 μm, p = 0.0001), kuyruk momenti (1.93 ± 2.36 ve 0.87 ± 1.03 μm, p = 0.013), kuyruk göçü (10.3 ± 12.24 ve 3.62  ± 2.75, p = 0.03) hastalarda istatistiksel olarak anlamlı düzeyde yüksek saptanmıştır.

Sonuç: Bulgularımız, PH hastalarında DNA tamir mekanizmaları devreye girmeden önce DNA hasarının artmış olabileceğini göstermektedir. DNA hasarı PH patofizyolojisine katkıda bulunabilir ve/veya PH'da yeni bir farmakolojik hedef olarak ele alınabilir.

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Birincil Dil tr
Konular Fizyoloji
Yayımlanma Tarihi Eylül 2019
Bölüm Araştırma Makalesi
Yazarlar

Orcid: 0000-0002-8795-0185
Yazar: Emine Kılıç-Toprak (Sorumlu Yazar)
Kurum: PAMUKKALE ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0002-8452-923X
Yazar: Yalın Tolga Yaylalı
Kurum: PAMUKKALE ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0002-7562-0744
Yazar: Yasin Özdemir
Kurum: PAMUKKALE ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0002-6850-6281
Yazar: Vural Küçükatay
Kurum: PAMUKKALE ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0001-6395-7924
Yazar: Hande Şenol
Kurum: PAMUKKALE ÜNİVERSİTESİ, TIP FAKÜLTESİ
Ülke: Turkey


Orcid: 0000-0002-9336-0205
Yazar: Melek Bor-Küçükatay
Kurum: PAMUKKALE ÜNİVERSİTESİ, TIP FAKÜLTESİ

Tarihler

Yayımlanma Tarihi : 30 Eylül 2019

Bibtex @araştırma makalesi { patd557153, journal = {Pamukkale Tıp Dergisi}, issn = {}, eissn = {1308-0865}, address = {Pamukkale Üniversitesi Tıp Fakültesi Eğitim Blokları Kınıklı kampüsü 20070 Kınıklı, Denizli}, publisher = {Pamukkale Üniversitesi}, year = {2019}, volume = {12}, pages = {415 - 421}, doi = {10.31362/patd.557153}, title = {Pulmoner hipertansiyonlu hastalarda comet analizi ile değerlendirilen artmış DNA hasarı}, key = {cite}, author = {Kılıç-Toprak, Emine and Yaylalı, Yalın Tolga and Özdemir, Yasin and Küçükatay, Vural and Şenol, Hande and Bor-Küçükatay, Melek} }
APA Kılıç-Toprak, E , Yaylalı, Y , Özdemir, Y , Küçükatay, V , Şenol, H , Bor-Küçükatay, M . (2019). Pulmoner hipertansiyonlu hastalarda comet analizi ile değerlendirilen artmış DNA hasarı. Pamukkale Tıp Dergisi , 12 (3) , 415-421 . DOI: 10.31362/patd.557153
MLA Kılıç-Toprak, E , Yaylalı, Y , Özdemir, Y , Küçükatay, V , Şenol, H , Bor-Küçükatay, M . "Pulmoner hipertansiyonlu hastalarda comet analizi ile değerlendirilen artmış DNA hasarı". Pamukkale Tıp Dergisi 12 (2019 ): 415-421 <https://dergipark.org.tr/tr/pub/patd/issue/48923/557153>
Chicago Kılıç-Toprak, E , Yaylalı, Y , Özdemir, Y , Küçükatay, V , Şenol, H , Bor-Küçükatay, M . "Pulmoner hipertansiyonlu hastalarda comet analizi ile değerlendirilen artmış DNA hasarı". Pamukkale Tıp Dergisi 12 (2019 ): 415-421
RIS TY - JOUR T1 - Pulmoner hipertansiyonlu hastalarda comet analizi ile değerlendirilen artmış DNA hasarı AU - Emine Kılıç-Toprak , Yalın Tolga Yaylalı , Yasin Özdemir , Vural Küçükatay , Hande Şenol , Melek Bor-Küçükatay Y1 - 2019 PY - 2019 N1 - doi: 10.31362/patd.557153 DO - 10.31362/patd.557153 T2 - Pamukkale Tıp Dergisi JF - Journal JO - JOR SP - 415 EP - 421 VL - 12 IS - 3 SN - -1308-0865 M3 - doi: 10.31362/patd.557153 UR - https://doi.org/10.31362/patd.557153 Y2 - 2019 ER -
EndNote %0 Pamukkale Tıp Dergisi Pulmoner hipertansiyonlu hastalarda comet analizi ile değerlendirilen artmış DNA hasarı %A Emine Kılıç-Toprak , Yalın Tolga Yaylalı , Yasin Özdemir , Vural Küçükatay , Hande Şenol , Melek Bor-Küçükatay %T Pulmoner hipertansiyonlu hastalarda comet analizi ile değerlendirilen artmış DNA hasarı %D 2019 %J Pamukkale Tıp Dergisi %P -1308-0865 %V 12 %N 3 %R doi: 10.31362/patd.557153 %U 10.31362/patd.557153
ISNAD Kılıç-Toprak, Emine , Yaylalı, Yalın Tolga , Özdemir, Yasin , Küçükatay, Vural , Şenol, Hande , Bor-Küçükatay, Melek . "Pulmoner hipertansiyonlu hastalarda comet analizi ile değerlendirilen artmış DNA hasarı". Pamukkale Tıp Dergisi 12 / 3 (Eylül 2019): 415-421 . https://doi.org/10.31362/patd.557153
AMA Kılıç-Toprak E , Yaylalı Y , Özdemir Y , Küçükatay V , Şenol H , Bor-Küçükatay M . Pulmoner hipertansiyonlu hastalarda comet analizi ile değerlendirilen artmış DNA hasarı. Pamukkale Tıp Dergisi. 2019; 12(3): 415-421.
Vancouver Kılıç-Toprak E , Yaylalı Y , Özdemir Y , Küçükatay V , Şenol H , Bor-Küçükatay M . Pulmoner hipertansiyonlu hastalarda comet analizi ile değerlendirilen artmış DNA hasarı. Pamukkale Tıp Dergisi. 2019; 12(3): 421-415.