Investigation of erythrocyte membrane lipid profile, oxidative stress and dna damage parameters in patients with chronic lymphoid leukemia

Yıl 2025, Cilt: 18 Sayı: 1, 12 - 12

Bahadır Karakula , Gulsum Akgun Cagliyan , Başak Ünver Koluman , Mehmet Nuri Baser , Melek Tunç Ata , Fatih Altıntaş , Emine Kılıç Toprak , Ayşegül Çört

https://doi.org/10.31362/patd.1548844

Öz

Purpose: Chronic lymphocytic leukemia (CLL) is the most seen type of leukemia in adults. There are few biomarkes that are used for better understanding how oxidative stress is involved in the pathophysiology of hematologic malignency. We aimed to evaulate oxidative stress, DNA damage and erythrocyte membrane lipid profile in CLL patients in this study. The study is included 38 CLL patients and 38 age-sex matched controls.
Materials and method: Total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), DNA damage examination with Comet assay, serum 8-OHdG measurement and gas chromatographic analysis were performed between the case and control groups.
Results: It was observed that TOS and OSI values were higher in the case group than in the control group (p=0.014 and p=0.022, respectively). DNA damage measured by Comet method was found to be increased in the case group (p<0.05). Although erythrocyte membrane fatty acid levels were found to be decreased in the case group compared to the control group, no statistically significant difference was found (p>0.05).
Conclusion: It has been shown that CLL patients had higher oxidant capasity as consequence. Oxidative stress and DNA damage are increased in CLL patients in this study. It is early to evaulate on erythrocyte membrane lipid profile in CLL patients. However, the study can be ligthed the way future studies on the subject.

Anahtar Kelimeler

Oxidative stress, DNA damage, erythrocyte membrane lipid profile, chronic lymphocytic leukemia

Etik Beyan

Pamukkale University Faculty of Medicine Ethical Review Board approved this study (date: 31.05.2021, issue: 60116787-020-56364). We conducted all procedures involving human participants in accordance with the ethical standards of institutional and/or national research committees and the Declaration of Helsinki.

Destekleyen Kurum

This study is supported by funding from the Pamukkale University (project number 2021TIPF027).

Proje Numarası

This study is supported by funding from the Pamukkale University (project number 2021TIPF027).

Kronik lenfoid lösemili hastalarda eritrosit membran lipid profili, oksidatif stres ve DNA hasarı parametrelerinin araştırılması

Öz

Amaç: Kronik lenfositik lösemi (KLL), erişkinlerde en sık görülen lösemi türüdür. Hematolojik malignite patofizyolojisinde oksidatif stresin nasıl rol oynadığını daha iyi anlamak için kullanılan az sayıda biyobelirteç vardır. Bu çalışmada KLL hastalarında oksidatif stres, DNA hasarı ve eritrosit membran lipit profilini değerlendirmeyi amaçladık. Çalışmaya 38 KLL hastası ve yaş-cinsiyet uyumlu 38 kontrol dahil edildi.
Gereç ve yöntem: Olgu ve kontrol grupları arasında total oksidan durum (TOS), toplam antioksidan durum (TAS), oksidatif stres indeksi (OSI), Comet assay ile DNA hasarı incelemesi, serum 8-OHdG ölçümü ve gaz kromatografik analizi yapıldı.
Bulgular: TOS ve OSI değerlerinin olgu grubunda kontrol grubuna göre daha yüksek olduğu görüldü (sırasıyla p=0,014 ve p=0,022). Comet yöntemiyle ölçülen DNA hasarının olgu grubunda arttığı belirlendi (p<0,05). Olgu grubunda eritrosit membran yağ asidi düzeylerinde kontrol grubuna göre azalma saptanmasına rağmen istatistiksel olarak anlamlı fark bulunamadı (p>0,05).
Sonuç: Sonuç olarak KLL hastalarının oksidan kapasitesinin daha yüksek olduğu gösterilmiştir. Çalışmamızda KLL hastalarında oksidatif stres ve DNA hasarı artmıştır. KLL hastalarında eritrosit membran lipid profilini değerlendirmek için henüz erkendir. Ancak çalışmamız konuyla ilgili gelecekte yapılacak çalışmalara ışık tutabilir.

Anahtar Kelimeler

Oksidatif stress, DNA hasarı, eritrosit membran lipid profili, kronik lenfositik lösemi

Proje Numarası

This study is supported by funding from the Pamukkale University (project number 2021TIPF027).

Kaynakça

• Mukkamalla SKR, Taneja A, Malipeddi D, Master SR. Chronic Lymphocytic Leukemia. In: StatPearls. Treasure Island (FL): StatPearls Publishing; March 7, 2023.
• Lumsden AL, Rogers JT, Majd S, et al. Dysregulation of Neuronal Iron Homeostasis as an Alternative Unifying Effect of Mutations Causing Familial Alzheimer's Disease. Front Neurosci. 2018;12:533. Published 2018 Aug 13. doi:10.3389/fnins.2018.00533
• Chen X, Ji Y, Liu R, et al. Mitochondrial dysfunction: roles in skeletal muscle atrophy. J Transl Med. 2023;21(1):503. Published 2023 Jul 26. doi:10.1186/s12967-023-04369-z
• Chiorazzi N, Chen SS, Rai KR. Chronic Lymphocytic Leukemia. Cold Spring Harb Perspect Med. 2021;11(2):a035220. Published 2021 Feb 1. doi:10.1101/cshperspect.a035220
• Kipps TJ, Stevenson FK, Wu CJ, et al. Chronic lymphocytic leukaemia. Nat Rev Dis Primers. 2017;3:16096. Published 2017 Jan 19. doi:10.1038/nrdp.2016.96
• Talibov M, Auvinen A, Weiderpass E, et al. Occupational solvent exposure and adult chronic lymphocytic leukemia: No risk in a population-based case-control study in four Nordic countries. Int J Cancer. 2017;141(6):1140-1147. doi:10.1002/ijc.30814
• Zhevak T, Shelekhova T, Chesnokova N, et al. The relationship between oxidative stress and cytogenetic abnormalities in B-cell chronic lymphocytic leukemia. Exp Mol Pathol. 2020;116:104524. doi:10.1016/j.yexmp.2020.104524
• Collado R, Ivars D, Oliver I, et al. Increased oxidative damage associated with unfavorable cytogenetic subgroups in chronic lymphocytic leukemia. Biomed Res Int. 2014;2014:686392. doi:10.1155/2014/686392
• Sabry SA, El Senduny FF, Abousamra NK, Salah El Din M, Youssef MM. Oxidative stress in CLL patients leads to activation of Th9 cells: an experimental and comprehensive survey. Immunol Med. 2020;43(1):36-46. doi:10.1080/25785826.2019.1700747
• Capron C, Jondeau K, Casetti L, et al. Viability and stress protection of chronic lymphoid leukemia cells involves overactivation of mitochondrial phosphoSTAT3Ser727 [published correction appears in Cell Death Dis. 2015;6:e1627. Verhoyen, E [corrected to Verhoeyen, E]]. Cell Death Dis. 2014;5(10):e1451. Published 2014 Oct 9. doi:10.1038/cddis.2014.393
• Salimi A, Roudkenar MH, Sadeghi L, et al. Selective Anticancer Activity of Acacetin Against Chronic Lymphocytic Leukemia Using Both In Vivo and In Vitro Methods: Key Role of Oxidative Stress and Cancerous Mitochondria. Nutr Cancer. 2016;68(8):1404-1416. doi:10.1080/01635581.2016.1235717
• DE Rosa A, Zappavigna S, Villa MR, et al. Prognostic role of translocator protein and oxidative stress markers in chronic lymphocytic leukemia patients treated with bendamustine plus rituximab. Oncol Lett. 2015;9(3):1327-1332. doi:10.3892/ol.2014.2817
• D'Arena G, Vitale C, Perbellini O, et al. Prognostic relevance of oxidative stress measurement in chronic lymphocytic leukaemia. Eur J Haematol. 2017;99(4):306-314. doi:10.1111/ejh.12918
• Gaman AM, Buga AM, Gaman MA, Popa Wagner A. The role of oxidative stress and the effects of antioxidants on the incidence of infectious complications of chronic lymphocytic leukemia. Oxid Med Cell Longev. 2014;2014:158135. doi:10.1155/2014/158135
• Dong C, Zhang NJ, Zhang LJ. Oxidative stress in leukemia and antioxidant treatment. Chin Med J (Engl). 2021;134(16):1897-1907. Published 2021 Jul 12. doi:10.1097/CM9.0000000000001628
• Jelic MD, Mandic AD, Maricic SM, Srdjenovic BU. Oxidative stress and its role in cancer. J Cancer Res Ther. 2021;17(1):22-28. doi:10.4103/jcrt.JCRT_862_16
• Glei M, Schneider T, Schlörmann W. Comet assay: an essential tool in toxicological research. Arch Toxicol. 2016;90(10):2315-2336. doi:10.1007/s00204-016-1767-y
• Guedes Pinto T, de Souza DV, da Silva GN, Salvadori DMF, Martins MD, Ribeiro DA. Comet assay as a suitable biomarker for in vivo oral carcinogenesis: a systematic review. Biomarkers. 2023;28(8):692-702. doi:10.1080/1354750X.2023.2298182