Fruktoz ile indüklenen metabolik sendromda quercetinin adipokin profili üzerine etkileri

Yıl 2025, Cilt: 18 Sayı: 1, 11 - 11

Melek Tunç Ata , Emine Kılıç Toprak , Mehmet Başeğmez , Ayşegül Çört , Vural Küçükatay

https://doi.org/10.31362/patd.1586924

Öz

Amaç: Metabolik sendrom (MetS) obezite, insülin direnci, dislipidemi ve tip 2 diyabet (T2DM) ile karakterize bir sağlık durumudur. Bu çalışmada, Sprague Dawley sıçanlarında fruktoz ile indüklenen MetS üzerinde doğal bir flavonoid olan quercetinin etkilerinin değerlendirilmesi amaçlandı.
Gereç ve yöntem: 8-10 haftalık sıçanlar 4 gruba ayrıldı: kontrol (C) grubu, metabolik sendrom (MetS) grubu, kontrol+quercetin (C+Q) grubu ve metabolik sendrom+quercetin (MetS+Q) grubu. MetS gruplarına 10 hafta boyunca içme suyunda %20 fruktoz çözeltisi verildi. Çalışmanın son 4 haftasında Q verilen gruplardaki sıçanlara 50 mg/kg/vücut ağırlığı quercetin uygulandı. 10 hafta sonra, serum örnekleri Trigliserid (TG), Yüksek Yoğunluklu Lipoprotein (HDL), açlık insülini, resistin, (Interleukin 6) IL6, Tümör Nekroz Faktörü-alfa (TNF-α), leptin, C-Reaktif Protein (CRP) ve adiponektin (ADP) için ELISA kullanılarak test edildi. Vücut ağırlıkları, Lee indeksi ve HOMA-IR skorları da ölçüldü.
Bulgular: Fruktozla beslenen sıçanların vücut ağırlığında, Lee indeksinde, HOMA-IR skorlarında ve açlık insülininde kontrol grubuna kıyasla anlamlı artışlar, HDL'de ise anlamlı düşüşler görüldü. MetS grubunda ADP seviyeleri kontrol grubuna kıyasla anlamlı derecede düşüktü. MetS+Q grubunda, tedavi edilmeyen MetS grubuna kıyasla resistin, IL-6 ve leptin seviyelerinde azalma eğilimi vardı.
Sonuç: Bu bulgular, quercetinin MetS yönetiminde faydalı olabileceğini, ancak mekanizmalarını ve etkinliğini keşfetmek için daha fazla araştırmaya ihtiyaç olduğunu göstermektedir.

Anahtar Kelimeler

Adipokin, fruktoz, metabolik sendrom, quercetin

Etik Beyan

Bu çalışma Pamukkale Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu tarafından onaylanmıştır (PAUHDEK-2023/32-19.10.2023, 2023/06).

Destekleyen Kurum

Yazarlar bu çalışma için fon almamıştır.

Teşekkür

Yazarlar, istatistiksel analizlerdeki yardımlarından dolayı Yrd. Dr. Hande Şenol'a istatistiksel analizlerdeki yardımları için teşekkür eder.

Effects of quercetin on adipokine profile in fructose-induced metabolic syndrome

Öz

Purpose: Metabolic syndrome (MetS) is a health condition characterized by obesity, insulin resistance, dyslipidemia and type 2 diabetes (T2DM). This study aimed to assess the effects of quercetin, a natural flavonoid on MetS induced by fructose in Sprague Dawley rats.
Materials and methods: The rats, aged 8-10 weeks, were divided into 4 groups: control (C) group, metabolic syndrome (MetS) group, control+quercetin (C+Q) group, and metabolic syndrome+quercetin (MetS+Q) group. The MetS groups received a 20% fructose solution in drinking water for a duration of 10 weeks. For the last 4 weeks of the study, rats in the Q groups were administered 50 mg/kg/body weight quercetin. After 10 weeks, serum samples were tested using ELISA for Triglycerides (TG), High-Density Lipoprotein (HDL), fasting insulin, resistin, (Interleukin 6) IL6, Tumor Necrosis Factor-alpha (TNF-α), leptin, C-Reactive Protein (CRP) and, adiponectin (ADP). The body weights, Lee index and HOMA-IR scores were also measured.
Results: Fructose-fed rats showed significant increases in body weight, Lee index, HOMA-IR scores and, fasting insulin with significant decrease in HDL compared to controls. In MetS group, ADP levels were significantly lower compared to control group. In MetS+Q group, there was a tendency for reduced levels of resistin, IL-6, and leptin compared to the untreated MetS group.
Conclusion: These findings suggest that quercetin may be beneficial in managing MetS, though further research is needed to explore its mechanisms and effectiveness.

Anahtar Kelimeler

Adipokine, fructose, metabolic syndrome, quercetin

Etik Beyan

This study was approved by the Pamukkale University Animal Experiments Local Ethics Committee (PAUHDEK-2023/32-19.10.2023, 2023/06).

Destekleyen Kurum

The authors did not receive funding for this study.

Teşekkür

The authors thank Assist. Prof. Hande Senol for her help in statistical analyses.

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