Quorum Sensing Inhibitory Activities of Alpha-Pinene, Terpineol, Cineole, Linalool and Camphor

Yıl 2016, , 558 - 565, 25.07.2016

Seyhan Ulusoy , Erdi Doğru

https://doi.org/10.19113/sdufbed.16945

Öz

Many pathogenic Gram negative bacteria often use N-Acyl homoserine lactone mediated cell to cell communication (quorum sensing) system to control the gene expression of various virulence factors. Thus blocking or manipulating this system makes it an attractive target for discovery of new therapeutics. There are a number of plant-based natural products as quorum sensing inhibitors.. In this study, QS inhibitory potential of laurus, salvia, myrtus and juniperus essential oils were screened and compositions of oils were analysed by GC-MS. The anti-quorum sensing activity of alpha pinene, terpineol, cineole, linalool and camphor (major components of oils) tested against using Chromobacterium violaceum strain CV026, C. violaceum strain VIR07, Pseudomonas aeruginosa PAO1, P. aeruginosa PAC-1. Results showed that alpha pinene, terpineol, cineole, linalool and camphor significantly inhibited violacein production in C. violaceum 026, C. violaceum VIR07, as well as the elastase, pyocyanin production, swarming motilty in P. aeruginosa PAO1, P. aeruginosa PAC-1. Our findings demonstrated that alpha pinene, terpineol, cineole, linalool and camphor have potential as inhibitors of quorum sensing system in C violaceum and P. aeruginosa.

Anahtar Kelimeler

Pseudomonas aeruginosa, Quorum sensing, Alpha pinene, Terpineol, Cineole, Linalool

Alfa-Pinen, Terpineol, Sineol, Linelol ve Kamforun Bakteriyel Hücreler Arası İletişim Sistemi Üzerine İnhibitör Etkileri

Öz

Pek çok Gram negatif patojen bakteri çeşitli virülens faktörlerinin üretiminin genetik seviyede kontrolünü N-Açil homoserin lakton aracılı hücreler arası iletişim sistemini kullanarak sağlar. Bu nedenle bu hücreler arası iletişim sisteminin engellenmesi veya modifiye edilmesi, bu sistemi yeni terapötiklerin keşfi için cazip bir hedef haline getirmektedir. Hücreler arası iletişim sistemi inhibitörü, bitkisel kökenli pek çok doğal ürün bulunmaktadır. Bu çalışmada defne, adaçayı, mersin ve ardıç uçucu yağlarının bakterilerdeki hücreler arası iletişim sistemi üzerine engelleyici özellikleri incelenmiş ve bu yağ örneklerinin majör bileşenleri GC-MS ile belirlenmiştir. Alfa pinen, terpineol, sineol, linelol ve kamforun (yağ örneklerinin majör bileşenleri) hücreler arası iletişim sistemi üzerine inhibitör etkileri C. violaceum, 026, C. violaceum VIR07, Pseudomonas aeruginosa PAO1, P. aeruginosa PAC-1 kullanılarak araştırılmıştır. Sonuçlar alfa pinen, terpineol, sineol, linelol ve kamforun C. violaceum, 026, C. violaceum VIR07 suşunda viyolasin üretiminin hem de, P. aeruginosa PAO1 ve P. aeruginosa PAC-1 klinik izolatında piyosiyanin, elastaz üretimi ve kayma hareketini önemli derecede inhibe ettiğini göstermiştir. Çalışmanın sonuçları alfa pinen, terpieneol, sineol, linelol ve kamforun, C violaceum ve P. aeruginosa için hücreler arası iletişim sistemi üzerine inhibitör etkileri bulunduğu tespit edilmiştir.

Anahtar Kelimeler

Pseudomonas aeruginosa, Hücreler arası iletişim, Alfa pinen, Terpineol, Sineol, Linelol

Kaynakça

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