Structural Modelling and Structure-Function Analysis of Zymomonas mobilis Levansucrase

Burcu Kaplan-türköz; Bahar Bakar

doi:10.19113/sdufbed.81065

Structural Modelling and Structure-Function Analysis of Zymomonas mobilis Levansucrase

Öz

Levansucrases are bacterial enzymes which produce fructan polymers from sucrose via hydrolysis and transfructosylation activities. These polymers; levan and fructooligosaccharides are valuable for food and pharmaceutical industries. Levansucrases from Gram-positive bacteria such as Bacillus subtilis tend to produce levan, while those from Gram-negative bacteria preferentially produce fructooligosaccharides. Zymomonas mobilis is an efficient levansucrase producer and its extracellular levansucrase can produce both fructooligosaccharides and levan depending on the reaction parameters. In this study, the structure of Z. mobilis levansucrase was modeled in order to help to understand the structure-function relationship of the enzyme. Furthermore, amino acids previously reported to be important for levansucrase activity were mapped on the model. The structural model presents a five-bladed propeller with a deep, negatively charged central pocket, similar to other bacterial levansucrases. Mapping showed that amino acids which previously reported to affect fructan length are located on the periphery of the structure covering the active site central pocket. Thus it is showed that, for the first time, that hydrolysis and transfructosylation reactions are catalyzed on different parts of Z. mobilis levansucrase structure. The structural location of the critical amino acids will pave the way to identify other residues which control fructan length by site directed mutagenesis without altering the overall fold of the enzyme.

Anahtar Kelimeler

Levansucrase,Zymomonas mobilis; Protein structure modelling; Structural bioinformatics

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

-

Yazarlar

Burcu Kaplan-türköz

Bahar Bakar Bu kişi benim

Yayımlanma Tarihi

23 Mart 2017

Gönderilme Tarihi

13 Aralık 2016

Kabul Tarihi

-

Yayımlandığı Sayı

Yıl 2017 Cilt: 21 Sayı: 1

DOI

https://doi.org/10.19113/sdufbed.81065

IZ

https://izlik.org/JA72SS76MJ

Kaynak Göster

RIS / Bibtex

APA

Kaplan-türköz, B., & Bakar, B. (2017). Structural Modelling and Structure-Function Analysis of Zymomonas mobilis Levansucrase. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 21(1), 279-285. https://doi.org/10.19113/sdufbed.81065

AMA

1.Kaplan-türköz B, Bakar B. Structural Modelling and Structure-Function Analysis of Zymomonas mobilis Levansucrase. Süleyman Demirel Üniv. Fen Bilim. Enst. Derg. 2017;21(1):279-285. doi:10.19113/sdufbed.81065

Chicago

Kaplan-türköz, Burcu, ve Bahar Bakar. 2017. “Structural Modelling and Structure-Function Analysis of Zymomonas mobilis Levansucrase”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 21 (1): 279-85. https://doi.org/10.19113/sdufbed.81065.

EndNote

Kaplan-türköz B, Bakar B (01 Nisan 2017) Structural Modelling and Structure-Function Analysis of Zymomonas mobilis Levansucrase. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 21 1 279–285.

IEEE

[1]B. Kaplan-türköz ve B. Bakar, “Structural Modelling and Structure-Function Analysis of Zymomonas mobilis Levansucrase”, Süleyman Demirel Üniv. Fen Bilim. Enst. Derg., c. 21, sy 1, ss. 279–285, Nis. 2017, doi: 10.19113/sdufbed.81065.

ISNAD

Kaplan-türköz, Burcu - Bakar, Bahar. “Structural Modelling and Structure-Function Analysis of Zymomonas mobilis Levansucrase”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 21/1 (01 Nisan 2017): 279-285. https://doi.org/10.19113/sdufbed.81065.

JAMA

1.Kaplan-türköz B, Bakar B. Structural Modelling and Structure-Function Analysis of Zymomonas mobilis Levansucrase. Süleyman Demirel Üniv. Fen Bilim. Enst. Derg. 2017;21:279–285.

MLA

Kaplan-türköz, Burcu, ve Bahar Bakar. “Structural Modelling and Structure-Function Analysis of Zymomonas mobilis Levansucrase”. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, c. 21, sy 1, Nisan 2017, ss. 279-85, doi:10.19113/sdufbed.81065.

Vancouver

1.Burcu Kaplan-türköz, Bahar Bakar. Structural Modelling and Structure-Function Analysis of Zymomonas mobilis Levansucrase. Süleyman Demirel Üniv. Fen Bilim. Enst. Derg. 01 Nisan 2017;21(1):279-85. doi:10.19113/sdufbed.81065

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