Physical and Chemical Properties of Spirulina platensis Added Yoghurts in Different Concentrations

Yıl 2020, , 553 - 565, 25.12.2020

Sümeyra Aydemir , Zübeyde Öner

https://doi.org/10.19113/sdufenbed.534480

Cited By: 2

Öz

In this study, the effect of four different concentrations (0.25, 0.50, 0.75 and %1) of the blue-green algae species Spirulina platensis on the physical, chemical, microbiological and sensory properties of the yogurt were investigated during storage time. It was determined that there was no significant difference in some of the chemical analysis results in the set yoghurts produced using Spirulina platensis. However, the effect of Spirulina platensis use on dry matter, fat and titratable acidity values during 21 days of storage was found to be statistically significant (p <0.05). On the basis of microbiological analysis, the addition of Spirulina platensis, Streptococcus thermophilus and Lactobacillus delbruecki subps. bulgaricus numbers were not statistically significant (p> 0.05). Total phenolic content and total antioxidant capacity of yogurts prepared with Spirulina platensis were higher than control yogurt. Protein values also increased with the addition of Spirulina platensis compared to control yogurt. According to the results of the sensory analysis, while the highest score was taken the control yoghurt, it was followed by %0.25 Spirulina platensis added yoghurt. All these results indicated that Spirulina platensis can be used to improve the chemical properties of the set type yogurt.

Anahtar Kelimeler

Spirulina platensis, Yogurt, Functional product

Farklı Konsantrasyonlarda Spirulina platensis Eklenmiş Yoğurtların Kimyasal ve Mikrobiyolojik Özellikleri

Öz

Bu çalışmada, dört farklı konsantrasyonda (0,25, 0,50, 0,75 ve %1) mavi-yeşil alg türü olan Spirulina platensis’in yoğurdun içine katılmasıyla, yoğurdun fiziksel, kimyasal, mikrobiyolojik ve duyusal özellikleri üzerindeki etkisi depolama süresi boyunca incelenmiştir. Spirulina platensis kullanılarak üretilen set tipi yoğurtlarda, kimyasal analiz sonuçlarının bazılarında önemli bir farklılık bulunmadığı belirlenmiştir. Ancak yoğurt üretiminde Spirulina platensis kullanımının 21 günlük depolama süresi boyunca kuru madde, yağ ve titrasyon asitliği değerleri üzerindeki etkisi istatistiki olarak önemli bulunmuştur (p<0,05). Mikrobiyolojik analiz sonuçlarına bakıldığında ise Spirulina platensis ilavesinin, Streptococcus thermophilus ve Lactobacillus delbruecki subps. bulgaricus sayılarını istatistiki açıdan etkilemediği görülmüştür(p>0,05). Spirulina platensis ile hazırlanan yoğurtların, toplam fenolik madde miktarı ve toplam antioksidan kapasite değeri kontrol yoğurdundan daha yüksek bulunmuştur. Spirulina platensis ilavesi yoğurtlardaki protein miktarını arttırmıştır. Duyusal analiz sonuçlarına göre ise, panelistler tarafından en yüksek puanı kontrol yoğurdu alırken bunu %0,25 Spirulina platensis katkılı yoğurt örneği izlemiştir. Tüm bu sonuçlar, set tipi yoğurtların kimyasal özelliklerinin geliştirilmesi için Spirulina platensis’in kullanılabileceğini göstermiştir.

Anahtar Kelimeler

Spirulina platensis, Yoğurt, Fonksiyonel ürün

Kaynakça

• [1] Anonim, 2009. Türk Gıda Kodeksi Fermente Süt Ürünleri Tebliği, T.C. Gıda Tarım ve Hayvancılık Bakanlığı, Gıda ve Kontrol Genel Müdürlüğü, Tebliğ No:2009/25, Ankara.
• [2] Akın, N. 2006. Modern Yoğurt Bilimi ve Teknolojisi, Selçuk Üniversitesi Ziraat Fakültesi Gıda Mühendisliği Bölümü, Konya, 456s.
• [3] McGill, C.R., Fulgoni, V.L., DiRienzo, D., Huth, P.J., Kurilich, A.C. and Miller, G.D. 2008. Contribution of dairy products to dietary potassium intake in the United States population. The Journal of the American College of Nutrition, 27, 44-50.
• [4] Çakmakçı, S., Gündoğdu, E. 2005. Yoğurdun yararları ne kadar tekrarlansa yine de az. Hasad Gıda, 20, 10-15.
• [5] McKinley, M.C. 2005. The nutrition and health benefits of yoghurt. International Journal of Dairy Technology, 58, 1-12.
• [6] Özden, A, 2009. İnsan beslenmesinde yoğurdun yararlı etkileri. Güncel Gastroenteroloji Dergisi, 13(4), 227-231.
• [7] Dal Bello, B., Torri, L., Piochi, M., Zeppa, G. 2015. Healthy yogurt fortified with n-3 fatty acids from vegetable sources. Journal of Dairy Science, 98(12), 8375-8385.
• [8] Ghorbanzade, T., Jafari, S.M., Akhavan, S., Hadavi. R. 2017. Nano-encapsulation of fish oil in nano-liposomes and its application in fortification of yogurt. Food Chemistry 216, 146–152.
• [9] Öztürkoğlu-Budak, S., Akal, C., Yetişemiyen, A. 2016. Effect of dried nut fortification on functional, physicochemical, textural, and microbiological properties of yogurt. Journal of Dairy Science, 99(11), 8511-8523.
• [10] Robertson, R.C., Mateo, M.R.G., O'Grady, M.N., Guihéneuf F., Stengel, D.B., Ross, R.P., Fitzgerald, G.F., Kerry, J.P., Stanton C. 2016. An assessment of the techno-functional and sensory properties of yoghurt fortified with a lipid extract from the microalga Pavlova lutheri. Innovative Food Science and Emerging Technologies, 37, 237–246.
• [11] Ciferri, O., Tiboni, O. 1985. The Biochemistry and Industrial Potential of Spirulina. Annual Review of Microbiology, 39, 503-526.
• [12] Pinero Estrada, J.E., Bermejo Bescos, P., Villar del Fresno, A.M. 2001. Antioxidant Activity of Different Fractions of Spirulina platensis Protean Extract. Farmaco, 56(5-7), 497-500.
• [13] Rossi, N., Derouiniot-Chaplain, M., Jaouen, P., Legentilhomme, P., Petit, I. 2008. Arthrospira platensis Harvesting with Membranes: Fouling Phenomenon with Limiting and Critical Flux. Bioresour Technology, 99(14), 6162-6167.
• [14] Karkos, P.D., Leong, S.C., Karkos, C.D., Sivaji, N., Assimakopoulos, D.A. 2011. Spirulina in Clinical Practice: Evidence-Based Human Applications. Evidence-Based Complementary and Alternative Medicine, 2011, 1-4.
• [15] World Health Organization, 2004. WHO guidelines on safety monitoring of herbal medicines in pharmacovigilance systems. World Health Organization, 82s.
• [16] Gökpınar, Ş., Işık, O., Göksan, T., Durmaz, Y., Uslu, L., Ak, B., Önalan, S.K.,Akdoğan, P. 2013. Algal Biyoteknoloji Çalışmaları, Yunus Araştırma Bülteni, (4), 21-26.
• [17] Belay A. 2002. The Potential Application of Spirulina (Arthrospira) as a Nutritional and Therapeutic Supplement in Health Management. JANA, 5 (2), 27-48.
• [18] Shin, Y. M., Son, C. W., Sim, H. J., Kim, M. H., Kim, M. Y., Kwon, O. Y., Kim, M. R. 2008. Quality characteristics and antioxidant activity of Spirulina added yogurt. Korean journal of food and cookery science, 24(1), 68-75.
• [19] Agustini, T.W., Soetrisnanto, D. and Ma’ruf, W.F. 2016. Study on chemical, physical, microbiological and sensory of yoghurt enriched by Spirulina platensis. International Food Research Journal, 24(1), 367-371.
• [20] Barkallah, M., Dammak, M., Louati, I., Hentati, F., Hadrich,B., Mechichi, T., Ayadi, M.A., Fendri, İ., Attia, H., Abdelkafi, S. 2017. Effect of Spirulina platensis fortification on physicochemical, textural, antioxidant and sensory properties of yogurt during fermentation and storage. LWT - Food Science and Technology, 84, 323-330.
• [21] Dave, R.I., Shah, N.P. 1997. Viability of yoghurt and probiotic bacteria in yoghurts made from commercial starter cultures. International Dairy Journal, 7, 31-41.
• [22] Anonim, 1999. T.S.1330, Yoğurt Standardı. (Türk Standartları Enstitüsü), Ankara.
• [23] AOAC. 1990. Official Methods of Analysis, 15th Ed., Association of Official Analysis Chemists: Arlington, VA, USA.
• [24] Anonim, 1990. Türk Standartları Enstitüsü, TS 8189, Sütte Yağ Tayini-Gerber Metodu.
• [25] Öner, Z., Karahan, A. G., Aydemir, S., Aloğlu, H. Ş. 2008. Effect of transglutaminase on physicochemical properties of set-style yogurt. International Journal of Food Properties, 11(1), 196-205.
• [26] Kahyaoğlu, T., Kaya, S., Kaya, A. 2005. Effects of fat reduction and curd dipping temperature on viscoelasticity, texture and appearance of Gaziantep Cheese. Food Science Technology International, 11(3), 191–198.
• [27] Selçuk, A.R., Yılmaz, Y. 2009. İşlenmiş uzum cekirdeği tozu ilavesinin lokum benzeri bir urunun toplam fenolik madde iceriği ile antioksidan aktivitesi uzerine etkisi. Akademik Gıda, 7 (5), 56-61.
• [28] De Man, J. C., Rogosa, D., Sharpe, M. E. 1960. A medium for the cultivation of lactobacilli. Journal of applied Bacteriology, 23(1), 130-135.
• [29] Terzaghi, B. E. ve Sandine, W. E. 1975. Improved medium for lactic streptococci and their bacteriophages Applied and Environmental Microbiology Journal. 29(6), 807-813.
• [30] Varga, L., Sziget, J., Ördög, V. 1999. Effect of a Spirulina platensis biomass enriched with trace elements on combinations of starter culture strains employed in the dairy industry. Milchwissenschaft. 54(5), 247-248.
• [31] O'Sullivan, A.M., O'Grady, M.N., O'Callaghan, Y.C., Smyth, T.J., O'Brien, N.M., Kerry, J. P. 2016. Seaweed extracts as potential functional ingredients in yoğurt. Innovative Food Science and Emerging Technologies, 37, 293-299.
• [32] Sengupta, S., Koley, H., Dutta, S., Bhowal, J. 2018. Hypocholesterolemic effect of Spirulina platensis (SP) fortified functional soy yogurts on diet-induced hypercholesterolemia. Journal of Functional Foods, 48, 54-64.
• [33] Lupatini, A. L., Colla, L. M., Canan, C., Colla, E. 2017. Potential application of microalga Spirulina platensis as a protein source. Journal of the Science of Food and Agriculture, 97(3), 724-732.
• [34] Affan, M. A., Lee, D. W., Al-Harbi, S. M., Kim, H. J., Abdulwassi, N. I., Heo, S. J., Kang, D. H. 2015. Variation of Spirulina maxima biomass production in different depths of urea-used culture medium. Brazilian Journal of Microbiology, 46(4), 991–1000.
• [35] Santos, T.D., de Freitas, B.C.B., Moreira, J.B., Zanfonato, K., Costa, J.A.V. 2016. Development of powdered food with the addition of Spirulina for food supplementation of the elderly population. Innovative Food Science and Emerging Technologies, 37, 216-220.
• [36] Brignac, B., Aryana, K. J. 2012. Influence of various antioxidants on the characteristics of plain yogurt. Food and Nutrition Sciences, 3, 1277–1280.
• [37] El-Baky, H.H.A., El Baz, F.K., El-Baroty, G.S. 2009. Enhancement of antioxidant production in Spirulina platensis under oxidative stress. Acta Physiologiae Plantarum, 31(3), 623-631.
• [38] Ismaiel, M.M. S., El-Ayouty, Y.M., Piercey-Normore, M. 2016. Role of pH on antioxidants production by Spirulina (Arthrospira) platensis. Brazilian journal of microbiology, 47(2), 298-304.
• [39] Goiris, K., Muylaert, K., Fraeye, I., Foubert, I., De Brabanter, J., De Cooman, L. 2012. Antioxidant potential of microalgae in relation to their phenolic and carotenoid content. Journal of Applied Phycology, 24, 1477-1486.
• [40] Beheshtipour, H., Mortazavian, A.M., Haratian, P., Darani, K.K. 2012. Erratum to: Effects of Chlorella vulgaris and Spirulina platensis addition on viability of probiotic bacteria in yogurt and its biochemical properties. European Food Research and Technology, 235(4), 719-728.
• [41] Li, H.B., Cheng., K.W., Wong, C.C., Fan, K.W., Chen, F.Y. 2007. Jiang Antioksidan kapasitenin değerlendirilmesi ve seçilen mikroalglerin farklı fraksiyonlarının toplam fenolik içeriği. Food Chemistry, 102, 771-776.
• [42] O'Sullivan, A.M., O'Callaghan, Y.C., O'Grady, M.N., Waldron, D.S., Smyth, T. J., O'Brien, N.M., Kerry, J.P. 2014. An examination of the potential of seaweed extracts as functional ingredients in milk. International Journal of Dairy Technology, 67, 182–193.
• [43] Dönmez, Ö., Mogol, B. A., Gökmen, V. 2017. Syneresis and Rheological Behaviors of Set Yogurt Containing Green Tea and Green Coffee Powders. Journal of Dairy Science, 100(2), 901–907.
• [44] Şimşek, B., Gün, İ., Çelebi, M. 2010. Isparta Yöresinde Üretilen Süzme Yoğurtların Protein Profilleri ve Bunların Kimyasal Özelliklerle İlişkisi. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 20(3), 208–213.
• [45] Güldaş, M., İrkin, R. 2010. Influence of Spirulina platensis powder on the microflora of yoghurt and acidophilus milk. Mljekarstvo, 60(4), 237-243.
• [46] Akalın, A. S., Ünal, G., Dalay, M. C. 2009. Influence of Spirulina platensis biomass on microbiological viability in traditional and probiotic yogurts during refrigerated storage. Italian Journal of Food Science, 21(3), 357-364.
• [47] Fadei, V., Mohammad-Alasti, F., Khosravi-Darani. K. 2013. Influence of Spirulina platensis powder on the starter culture viability in probiotic yoghurt containing spinach during cold storage. European Journal of Experimental Biotechnology 3(3), 389–393.
• [48] Mocanu, G., Botez, E., Nistor, O. V., Andronoiu, D. G., Vlăsceanu, G. 2013. Influence of Spirulina platensis biomass over some starter culture of lactic bacteria. Journal of Agroalimentary Processes and Technologies, 19(4), 474-479.
• [49] Varga, L., Szigeti, J., Kovács, R., Földes, T., Buti, S. 2002. Influence of a Spirulina platensis biomass on the microflora of fermented ABT milks during storage (R1). Journal of Dairy Science, 85(5), 1031-1038.
• [50] Malik, P. 2011. Utilization of Spirulina powder for enrichment of ice cream and yoghurt. Karnataka Veterinary, Animal and Fisheries Sciences University, Doctoral dissertation, 151s.
• [51] Shimamatsu, H. 2004. Mass production of Spirulina, an edible microalga. Hydrobiologia, 512(1-3), 39-44.
• [52] Malik, P., Kempanna, C., Aman, P. 2013. Quality characteristics of ice cream enriched with Spirulina powder. International Journal of Food and Nutrition Science, 2(1), 44-50.