Distributaion of AIA(Artocarpus integrifolia) and PSA (Pisum sativum) Lectins in Bronchus, Bronchulus and Alveolar Cells in Sheep (Ovis aries) Respiratory System

Öz

In this study it was aimed to determination of immunohistochemical localization of primary, secondary, tertiary bronchus, bronchulus verus, bronchulus respiratorius of sheep (Ovis aries) with lectins which are very important potential and value in the study of glycoproteins, recognizing and binding carbohydrates specifically and reversibly, mediators of many physiological and pathological processes AIA and PSA. In conclusion, since the respiratory mucosa was always exposed to infectious agents it was found that there were some protection systems in the sheep lung and these systems are localized specifically for in the apical cytoplasm of the cells of the kinociliary cells that reach the lumen in the primary bronchus, on the surface of the lamina epithelial cells of the lumen (basal) cells, in the apical cytoplasm of the cells of the serous gland epithelium in the primary bronchus, in the (basal) cells of the tertiary bronchus and in the apical cytoplasm of the cells reaching the lumen, on the lamina epithelial surface of the bronchulus verus, on the surface of bronchus respiratory tract lamina epithelial cells, galactose/galactosamine sugar residues in alveolar macrophages and lamina epithelial surface of primary bronchulus are speculated for mannose/glucose sugar residues, which will contribute to animal agriculture.

Anahtar Kelimeler

• Sheep,Ovis aries,Bronchus,Bronchulus,Immunohistochemistry,Lectin

Koyun (Ovis aries) Solunum Sistemindeki Bronkus, Bronkulus ve Alveoler Hücrelerdeki AIA (Artocarpus integrifolia) ve PSA (Pisum sativum) Lektinlerinin Dağılımı

Öz

Bu çalışmada karbonhidratları spesifik ve geri dönüşümlü olarak tanıyan ve bağlanan, glikoproteinlerin çalışılmasında çok önemli bir potansiyel ve değere sahip, birçok fizyolojik ve patolojik süreçlerin anlaşılmasında aracı olan lektinlerden AIA ve PSA’ nın koyun primer, sekonder, tersiyer bronkus, bronkulus verus ve bronkulus respiratoryuslarda immunohistokimyasal olarak lokalizasyonunun belirlenmesi amaçlandı. Sonuç olarak, solunum mukozası her zaman enfeksiyöz ajanlara maruz kaldığından, koyun akciğerinde bazı korunma sistemlerinin olduğu ve bu sistemlerden primer bronkuslarda lümene ulaşan kinosilyumlu hücrelerin apikal sitoplazmalarında, lümene ulaşmayan (bazal) hücrelerin lamina epitelyalis yüzeyinde, primer bronkuslarda seröz bez epiteline ait hücrelerin apikal sitoplazmalarında, tersiyer bronkusların lümene ulaşmayan (bazal) hücrelerinde ve lümene ulaşan hücrelerin apikal sitoplazmasında, bronkulus verusun ve bronkulus respiratoryusun lamina epitelyalis hücrelerinin yüzeyinde, alveolar makrofajlarda galaktoz/galaktozamin şeker rezidüeleri ve primer bronkusun lamina epitelyalis yüzeyinin mannoz/glukoz şeker rezidüeleri için spesifikleştiği, bunun da hayvan yetiştiriciliğine katkıda bulunacağı düşünülmektedir.

Anahtar Kelimeler

• Koyun,Ovis aries,Bronkus,Bronkulus,İmmunohistokimya,Lektin

Kaynakça

1. [1] Baykal, B. ed. 2014. Histoloji Konu Anlatımı ve Atlas. Palme Yayıncılık, Ankara, 676-678s.
2. [2] Abdı, K., Kozbık, L., Lı, X., Menızr, S. J. 1994. Membrane Glycoconjugates Expressed on Sheep Airway Epithelium. The journal of Histochemistry and Cytochemistry, 42(10), 1341-1347.
3. [3] Sumner, J. B., Howell, S. F. 1936. Identification of Hemagglutinin of Jack Bean with Concanavalin A. J Bacteriol, 32(2), 227–237.
4. [4] Landsteiner, K. 1962. The Specificity of Serological Reactions. Agglutination. Dover Publications, New york, 246s.
5. [5] Lam, S. K., Ng, T. B. 2011. Lectins: Production and Practical Applications. Applied Microbiology and Biotechnology, 89, 45–55.
6. [6] Vretblad, P. 1976. Purification of Lectins by Biospecific Affinity Chromatography. Biochimica et Biophysica Acta (BBA)-Protein Structure, 169–176.
7. [7] Dan, X., Liu, W., Ng, T. B. 2016. Development and Applications of Lectins as Biological Tools in Biomedical Research. Medicinal Research Reviews, 221–247.
8. [8] Ofek, I., Mirelman, D., Sharon, N. 1977. Adherence of Escherichia coli to Human Mucosal Cells Mediated by Mannose Receptors. Nature, 623–625.

9. [9] Sharon, N., Lis, H. 2004. History of Lectins: From Hemagglutinins to Biological Recognition Molecules. Glycobiology, 53–62.
10. [10] Islam, F., Gopalan, V., K-Y Lam, A., Kabir, S. R. 2018. Pea Lectin Inhibits Cell Growth by Inducing Apoptosis in SW480 and SW48 cell lines. International Journal of Biological Macromolecules. 1(117), 1050-1057.
11. [11] Gyimah, I. E., Panigraphy, B. 1988. Adhesin-Receptor Interactions Mediating the Attachment of Pathogenic Escherichia coli to Chicken Tracheal Epithelium. Avian Dis 32, 74-78.
12. [12] Dorscheid, D. R., Conforti, A. E., Hamann, K. J., Rabe, K. F., White, S. R. 1999. Characterizationof Cell Surface Lectin-Binding Patterns of Human Airway Epithelium. Histochemical Journal, 31(3), 145-151.
13. [13] Yi, S. M., Harson, R. E., Zabner, J., Welsh, M. J. 2001. Lectin Binding and Endocytosis at the Apical Surface of Human Airway Epitelia. Gene Therapy, 1826–1832.
14. [14] Chagnon, F., Fournier, C., Charette, P. G., Moleski, L., Payet, M. D., Dobbs, L. G., Lesur, O. 2010. In Vivo Intravital Endoscopic Confocal Fluorescence Microscopy of Normal and Acutely Injured Rat Lungs. Laboratory Investigation, 90, 824–834.
15. [15] Ibrahım, D., Nakamuta, N., Tanıguchı, K., Yamamoto, Y., Tanıguchı, K. 2014. Histological and Lectin Histochemical Studies on the Olfactory and Respiratory Mucosae of the Sheep. Journal of Veterenary Medical Science. 76(3), 339–346.
16. [16] Meyer, K. C., Powers, C., Rosentha, N., Auerbach, R. 1993. Alveolar Macrophage Surface Carbohydrate Expression Is Altered in Interstitial Lung Disease as Determined by Lectin-binding Profiles. Amerıcan Revıew Of Respıratory Dısease, 1325-1334.
17. [17] Barkhordari, A., Stoddart, W., McClure, F., McClure, J. 2004. Lectin Histochemistry of Normal Human Lung. Journal of Molecular Histology 35(2), 147-156.