Burdur-Isparta Yöresinde Yayılış Gösteren Bazı Lathyrus Taksonlarının Radikal Süpürücü Aktivitesi

Öz

Burdur-Isparta yöresinde yayılış gösteren Lathyrus aphaca L. var. pseudoaphaca (Boiss.) Davis, L. aureus (Stev.) Brandza, L. cicera L., L. sphaericus Retz., L. digitatus (Bieb.) Fiori in Fiori & Paol. and L. setifolius L. taksonlarına ait ekstraktların radikal süpürücü aktivitesi belirlenmiştir. Kurutulmuş topraküstü kısımları ve tohumlarının metanol ekstraktlarından klorofil ve lipofilik bileşikler uzaklaştırılmıştır. Sulu ve metanol ekstraktlarının 2,2-difenil-1-pikril hidrazil, süperoksit ve nitrik oksit radikali süpürücü aktiviteleri araştırılmıştır. Ayrıca gallik aside eşdeğer toplam fenolik madde miktarı Folin-Ciocalteu reaktifi kullanılarak belirlenmiştir. En yüksek 2,2-difenil-1-pikril hidrazil radikali süpürücü aktivite L. aphaca var. pseudoaphaca’nın topraküstü kısımlarında, en yüksek toplam fenolik madde içeriği gallik aside eşdeğer olarak L. sphaericus’un tohumlarında bulunmuştur.

Anahtar Kelimeler

• DPPH
• Lathyrus
• NO
• Radikal süpürücü aktivite
• SO
• Toplam fenolik içeriği

Radical Scavenging Activity of Some Lathyrus Taxa Distributed in Burdur-Isparta Regio

Öz

Radical scavenging activity of the extracts of Lathyrus aphaca L. var. pseudoaphaca (Boiss.) Davis, L. aureus (Stev.) Brandza, L. cicera L., L. sphaericus Retz., L. digitatus (Bieb.) Fiori in Fiori & Paol. and L. setifolius L. taxa distributed in Burdur-Isparta regio was determined. Methanol extracts of air dried aerial parts and the seeds of the plants were dissolved in water and chlorophylls and lipophilic compounds were removed from the aqueous extracts. 2,2-diphenyl-1-picryl hydrazyl, superoxide and nitric oxide radical scavenging activity of aqueous and methanol extracts of the plants were tested. Gallic acid equivalent total phenolic contents of the plant extracts were also detected using Folin-Ciocalteu reagent. According to the 2,2-diphenyl-1-picryl hydrazyl radical scavenging activity test, the highest antioxidant activity was found in aerial parts of L. aphaca var. pseudoaphaca and highest total phenolic content was found as gallic acid equivalent in L. sphaericus seed extracts.

Anahtar Kelimeler

• DPPH
• Lathyrus
• NO
• Radical scavenging activity
• SO
• Total phenolic content

Kaynakça

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