Determination of Phytochemical Profile, Antiquorum Sensing and Antioxidant Activities of Tragopogon oligolepis

Öz

Objective: In this study it was aimed to examine antiquorum sensing, antioxidant activities by using root and aerial parts extracts of Tragopogon oligolepis. Also phenolic content was detected using HPLC analysis.

Material-Method: Antioxidant activity was detected by DPPH, FRAP methods and phenolic content HPLC. Antiquorum sensing activity was investigated by using pyocyanin and swarming motility assay on Pseudomonas aeruginosa PAO1.

Results: Phytochemical profile findings showed that 12 components were detected in the root and 10 components in the aerial parts. The main components were found chlorogenic acid and o-coumaric acid. According to the obtained of antioxidant levels the aerial parts extracts of T. oligolepis had the best antioxidant property in our results. The amount of DPPH (0.60 ± 0.01 mg/ml) and phenolic content (6.55±0.18 mg GAE/g sample) was determined to be high in the aerial parts. In the FRAP analysis, high reducing power was found in the roots (12.62±0.36 μmol FeSO4/g sample). According to these results, although T. oligolepis extracts do not reach very high amounts in terms of antioxidant results, it is thought to be a plant that can be evaluated in terms of removing oxidant effects. The results of antiquorum sensing activity showed that both root and aerial parts extract showed strong inhibitory effect on swarming motility 62%, %65 rate respectively.

Conclusions: Tragopogon oligolepis, an endemic species, can be evaluated as an antiquorum sensing inhibitor candidate with its phytochemical contents.

Anahtar Kelimeler

• DPPH
• FRAP
• Pseudomonas
• T. oligolepis

Tragopogon oligolepis’ın Fitokimyasal İçeriği, Antioksidan ve Antiquorum Sensing Özelliğinin Belirlenmesi

Öz

Amaç: Bu çalışmada, Tragopogon oligolepis'in kök ve toprak üstü kısım ekstraktları kullanılarak antiquorum sensing ve antioksidan aktivitelerinin incelenmesi amaçlanmıştır. Ayrıca HPLC analizi kullanılarak fenolik içerik tespit edilmiştir.

Gereç Yöntem: Antioksidan aktivite DPPH, FRAP yöntemleri ve fenolik içerik HPLC ile tespit edildi. Çevreyi algılama aktivitesi, Pseudomonas aeruginosa PAO1 üzerinde piyosiyanin ve kayma hareketi testi kullanılarak araştırıldı.

Bulgular: Fitokimyasal profil bulguları, kökte 12 bileşen ve toprak üstü kısımlarda 10 bileşen tespit edildiğini göstermiştir. Ana bileşenler klorojenik asit ve o-kumarik asit olarak bulunmuştur. Elde edilen antioksidan seviyelerine göre, sonuçlarımızda en iyi antioksidan özelliği T. oligolepis'in toprak üstü kısımları ekstreleri göstermiştir. Toprak üstü kısımlarda DPPH (0.60 ± 0.01 mg/mL) ve fenolik içerik (6.55±0.18 mg GAE/g numune) miktarının yüksek olduğu belirlendi. FRAP analizinde köklerde yüksek indirgeme tespit edildi (12.62±0.36 μmol FeSO4/g numune). Antiquorum sensing sonuçlarına göre ise kök ve toprak üstü ektraktları kayma hareketi üzerine %62 ve %65 oranında güçlü inhibisyon etki göstermiştir.

Sonuç: Elde edilen sonuçlar neticesinde endemik bir tür olan T. oligolepis, fitokimyasal içeriği ile antiquorum sensing inhibitör adayı olarak değerlendirilebilir.

Anahtar Kelimeler

• DPPH
• FRAP
• Pseudomonas
• T. oligolepis

Kaynakça

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