Biosynthesis of Ag Nanoparticle Using Catharanthus roseus Extract and Investigation of its Toxic Effect in Healthy Cell

Öz

Biosynthesis of nanoparticles from plant extracts is of great interest because it is inexpensive, environmentally friendly, and suitable for large-scale production compared to other synthesis methods. In this study, it was aimed to perform the synthesis and characterization of Ag nanoparticles (Ag NPs) by using Catharantus roseus (C. roseus) extract as a reducing and stabilizing agent and to evaluate their toxic effects against a healthy human cell line. The synthesis of Ag NPs were successfully carried out using the extract of C. roseus. By using FTIR, XRD, UV-Vis, and TEM analysis, the synthesized Ag NPs were characterized. According to XRD and TEM analysis results, it was observed that the average diameter of the synthesized Ag NPs was 16 ± 6 nm and its shape was a face-centered cubic (fcc). In addition, the toxic effects of biologically synthesized Ag NPs against a human healthy cell line (L929) were investigated in the study and the half-maximum inhibitory concentration (IC50) was found to be 2.909 µL/mL.

Anahtar Kelimeler

• Green synthesis
• Catharanthus roseus
• Ag nanoparticles
• Toxicity

Catharantus roseus Ekstratı Kullanarak Ag Nanopartikülünün Sentezlenmesi ve Sağlıklı İnsan Hücresinde Toksik Etkisinin İncelenmesi

Öz

Bitki ekstraktları kullanılarak nanoparçacıkların biosentezi, diğer sentez yöntemlerine kıyasla daha uygun maliyetli, çevre dostu ve büyük ölçekli üretim için uygun olması sebebiyle büyük ilgi görmektedir. Bu çalışmada indirgeyici ve stabilize edici ajan olarak Catharantus roseus (C. roseus) ekstraktı kullanılarak Ag nanopartiküllerin (Ag NPs) sentezinin ve karakterizasyonunun gerçekleştirilmesi ve sağlıklı insan hücresine karşı toksik etkilerinin incelenmesi hedeflenmiştir. Ag NPs sentezi C. roseus ekstraktı kullanılarak başarılı bir şekilde gerçekleştirilmiştir. Sentezlenen Ag NPs FTIR, XRD, UV-Vis ve TEM analizleri ile karakterize edilmiştir. XRD ve TEM analiz sonuçlarına göre, sentezlenen Ag NPs ortalama partikül boyutunun 16 ± 6 nm ve şeklinin yüzey merkezli kübik olduğu gözlenmiştir. Ayrıca çalışmada biyolojik olarak sentezlenmiş olan Ag NPs sağlıklı insan hücre hattına (L929) karşı toksik etkileri araştırılmış ve yarı maksimum inhibitör konsantrasyonu (IC50) 2.909 µL/mL olarak bulunmuştur.

Anahtar Kelimeler

• Yeşil kimya
• Catharanthus roseus
• Ag nanopartikülü
• Toksisite

Kaynakça

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