The Therapeutic Potency of Hesperis matronalis subsp. matronalis for Alzheimer's and Microbial Diseases

Öz

The goal of the present study, to investigate for the methanolic extract from the aerial parts of Hesperis matronalis subsp. matronalis in terms of acetylcholinesterase (AChE) and butyrylcholinesterase inhibitory and antimicrobial effects to detect therapeutic potential of Alzheimer's and microbial diseases. Alzheimer’s disease is a serious health problem because of its memory defect effect related with neurodegenerative damage. Microorganisms are associated with many ailments that cause negative effects on human health. Hesperis genus is represented by 28 genus in Turkey. Hesperis matronalis subsp. matronalis was selected for the study among from the genus. The inhibitory activities of AChE and BuChE of Hesperis matronalis subsp. matronalis were determined by colorimetric Ellman’s method and the antimicrobial activity was executed using agar diffusion method. As a result of the study, the antimicrobial activity of Hesperis matronalis subsp. matronalis was observed on Escherichia coli, Yersinia pseudotuberculosis, Klebsiella pneumonia subp. Pneumonia, Staphylococcus aureus, Enterococcus faecalis, Mycobacterium smegmatis, Bacillus cereus and Candida albicans. The IC50 values for inhibitory activities of AChE and BuChE of Hesperis matronalis subsp. matronalis have been found as 316.23 ± 2.15 and 74.13 ± 0.85 µg mL-1, respectively. Because of significiant inhibitory effects on AChE and BuChE and antimicrobial activity, Hesperis matronalis subsp. matronalis can be a candidate alternative natural drug source in the treatment of Alzheimer's and many microbial diseases.

Anahtar Kelimeler

• Antimicrobial activity
• cholinesterase inhibition
• Hesperis matronalis

Hesperis matronalis subsp. matronalis’in Alzheimer ve Mikrobiyal Rahatsızlıklar için Terapötik Potansiyeli

Öz

Bu çalışmanın amacı Hesperis matronalis subsp. matronalis’in topraküstü kısımlarının methanol ekstresinin, Alzheimer hastalığı ve mikrobiyal rahatsızlıklar için tedavi edici potansiyelinin belirlenebilmesi için, asetilkolinesteraz (AChE) ve butirilkolinesteraz (BuChE) inhibitor etkilerinin ve antimikrobiyal etkisinin ortaya çıkarılmasıdır. Alzheimer hastalığı, nörodejeneratif hasarla doğrudan ilişkili hafıza bozukluğuna yol açması nedeniyle ciddi bir sağlık sorunu olarak değerlendirilmektedir. Mikroorganizmalar insan sağlığı üzerine olumsuz etkilere yol açabilen, birçok rahatsızlıkla doğrudan ilişkilidir. Hesperis cinsi Türkiye’de 28 tür ile temsil edilmektedir. Hesperis cinsine ait türler arasından, çalışma için H. matronalis subsp. matronalis türü bu çalışma kapsamında seçilmiştir. H. matronalis subsp. matronalis’in AChE ve BuChE inhibitör aktivitesi kolorimetrik Ellman’ın yöntemiyle belirlenirken, antimikrobiyal aktivitesi ise agar difüzyon metodu kullanılarak belirlenmiştir. Çalışma sonucunda elde edilen veriler ışığında, Escherichia coli, Yersinia pseudotuberculosis, Klebsiella pneumonia subp. pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Bacillus cereus, Mycobacterium smegmatis, Candida albicans ve Saccharomyces cerevisiae mikroorganizmaları üzerinde H. matronalis subsp. matronalis’in antimikrobiyal aktivitesinin olduğu gözlenmiştir. Çalışma kapsamında H. matronalis subsp. matronalis’in AChE ve BuChE inhibitör aktivitesi için IC50 değerleri ise, sırasıyla 316.23 ± 2.15 ve 74.13 ± 0.85 µg mL-1 olarak bulunmuştur. H. matronalis subsp. matronalis AChE ve BuChE'nin inhibitör etkileri ve potansiyel antimikrobiyal aktivitesi nedeniyle, H. matronalis, subsp. matronalis Alzheimer’ın yanısıra birçok mikrobiyal hastalığın tedavisinde alternatif bir doğal ilaç kaynağı olabilir.

Anahtar Kelimeler

• Antimikrobiyal aktivite
• kolinesteraz inhibisyonu
• Hesperis matronalis

Kaynakça

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