THE ASSOCIATION BETWEEN GRADE OF PERIODONTITIS AND GCF LEVELS OF TNF-α AND MIP-1α: A PRELIMINARY STUDY

Öz

Objective The role of cytokines and chemokines in the pathogenesis of periodontitis indicates that tumor necrosis factor alpha (TNF-α) and macrophage inflammatory protein 1α (MIP1α) have crucial functions in the initiation and progression of periodontitis. However, the cytokine and chemokine profiles of different grades of periodontitis are still unclear, and no conclusive information has yet been reported on biomarkers associated with the progression rate of periodontitis. Thus, the aim of the present study was to estimate the gingival crevicular fluid (GCF) levels of MIP-1α and TNF-α in Grades A, B, and C and to evaluate their role as reliable biomarkers in determining the grade of periodontitis. Materials and Methods Individuals were divided into three groups according to their grade of periodontitis: individuals diagnosed as Stage IV periodontitis with Grade A (Grade A, n = 21), individuals diagnosed as Stage IV periodontitis with Grade B (Grade B, n = 21), and individuals diagnosed as Stage IV periodontitis with Grade C (Grade C, n = 21). Results Although there were no significant differences between groups in terms of mean TNF-α levels, the mean MIP- 1α level of Grade C was significantly higher than that of Grade B and Grade A. The mean MIP-1α level of Grade B was significantly higher than that of Grade A (p < 0.05, Kruskal–Wallis test). Conclusion MIP-1α could have clinical utility as a screening biomarker for the grade of periodontitis.

Anahtar Kelimeler

• Chemokines
• cytokines
• gingival crevicular fluid
• periodontitis

PERİODONTİTİS DERECESİ İLE DİŞETİ OLUĞU SIVISI TNF-α VE MIP-1α SEVİYELERİ ARASINDAKİ İLİŞKİNİN DEĞERLENDİRİLMESİ: ÖN ÇALIŞMA

Öz

Amaç Periodontitisin patogenezindeki sitokin ve kemokinlerin rolü,periodontitisin başlaması ve ilerlemesinde önemli fonksiyonlara sahip olan ile Tümör Nekroz Faktör alfa (TNF-α) ve Makrofaj İnflamatuar Protein 1α ile (MIP 1α) gösterilmiştir. Bununla birlikte, farklı periodontitis derecelerinin sitokin ve kemokin profilleri hala belirsizdir ve periodontitisin ilerleme hızı ile ilişkili biyobelirteçler hakkında henüz kesin bilgiler bildirilmemiştir. Bu nedenle, bu çalışmanın amacı Derece A, B ve C'deki dişeti oluğu sıvısındaki (DOS) MIP-1α ve TNF-α'nın düzeylerini tahmin etmek ve periodontitis derecesini belirlemede güvenilir biyobelirteçler olarak rollerini değerlendirmektir. Gereç ve Yöntem Bireyler periodontitis derecelerine göre Evre IV periodontitis tanısı alan ve Derece A (Derece A, n = 21), Evre IV periodontitis tanısı alan ve Derece B (Derece B, n =21) ve Evre IV periodontitis tanısı alan Derece C bireyler (Derece C, n = 21) olmak üzere üç gruba ayrıldı. Bulgular Ortalama TNF-α seviyeleri açısından gruplar arasında anlamlı bir fark olmamasına rağmen, Derece C’deki ortalama MIP-1α seviyesi Derece B ve Derece A'dan anlamlı derecede yüksekti. Derece B'deki ortalama MIP-1α seviyesi Derece A'dan önemli ölçüde daha yüksek (p < 0.05, Kruskal–Wallis testi) bulundu. Sonuç: MIP-1α, periodontitis derecesi için tanımlayıcı bir biyobelirteç olarak klinik kullanıma sahip olabilir.

Anahtar Kelimeler

• kemokin
• sitokin
• dişeti oluğu sıvısı ,
• periodontitis

Kaynakça

1. 1. Slots J. Periodontitis: facts, fallacies and the future. Periodontology 2000. 2017;75(1):7-23.
2. 2. Stadler A, Angst P, Arce R, Gomes S, Oppermann R, Susin C. Gingival crevicular fluid levels of cytokines/chemokines in chronic periodontitis: a meta-analysis. Journal of Clinical Periodontology. 2016;43(9):727-745.
3. 3. Afacan B, Öztürk VÖ, Paşalı Ç, Bozkurt E, Köse T, Emingil G. Gingival crevicular fluid and salivary HIF-1α, VEGF, and TNF-α levels in periodontal health and disease. Journal of Periodontology 2019;90(7):788-797.
4. 4. Afacan B, Keleş Yücel Z, Paşali Ç, Atmaca İlhan H, Köse T, Emingil G. Effect of non‐surgical periodontal treatment on gingival crevicular fluid hypoxia inducible factor‐1 alpha, vascular endothelial growth factor and tumor necrosis factor‐alpha levels in generalized aggressive periodontitis patients. Journal of Periodontology. 2020;91(11):1495-1502.
5. 5. Nisha K, Suresh A, Anilkumar A, Padmanabhan S. MIP-1α and MCP-1 as salivary biomarkers in periodontal disease. The Saudi Dental Journal. 2018;30(4):292-298.
6. 6. Miranda T, Figueiredo N, Figueiredo L, Silva H, Rocha F, Duarte P. Cytokine profiles of healthy and diseased sites in individuals with periodontitis. Archives of Oral Biology. 2020;120:104957.
7. 7. Tonetti M, Greenwell H, Kornman K. Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. Journal of Periodontology. 2018;89:S159-S172.
8. 8. Caton J, Armitage G, Berglundh T, Chapple I, Jepsen S, Kornman K et al. A new classification scheme for periodontal and peri-implant diseases and conditions - Introduction and key changes from the 1999 classification. Journal of Clinical Periodontology. 2018;45:S1-S8.

9. 9. Graetz C, Mann L, Krois J, Sälzer S, Kahl M, Springer C et al. Comparison of periodontitis patients’ classification in the 2018 versus 1999 classification. Journal of Clinical Periodontology. 2019;46(9):908-917.
10. 10. Karaaslan F, Dikilitaş A. The association between stage‐grade of periodontitis and sleep quality and oral health‒related quality of life. Journal of Periodontology. 2019;90(10):1133-1141.
11. 11. Silness J, Löe H. Periodontal Disease in Pregnancy II. Correlation Between Oral Hygiene and Periodontal Condition. Acta Odontologica Scandinavica. 1964;22(1):121-135.
12. 12. Löe H. The Gingival Index, the Plaque Index and the Retention Index Systems. Journal of Periodontology. 1967;38(6):610-616.
13. 13. Dietrich T, Ower P, Tank M, West N, Walter C, Needleman I et al. Periodontal diagnosis in the context of the 2017 classification system of periodontal diseases and conditions – implementation in clinical practice. British Dental Journal. 2019;226(1):16-22.
14. 14. Papapanou P, Sanz M, Buduneli N, Dietrich T, Feres M, Fine D et al. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. Journal of Periodontology. 2018;89:S173-S182.
15. 15. Shiau H, Reynolds M. Sex Differences in Destructive Periodontal Disease: A Systematic Review. Journal of Periodontology. 2010;81(10):1379-1389.
16. 16. Shiau H. Periodontal Disease in Women and Men. Current Oral Health Reports. 2018;5(4):250-254.
17. 17. Fine D, Markowitz K, Fairlie K, Tischio-Bereski D, Ferrandiz J, Godboley D et al. Macrophage Inflammatory Protein-1α Shows Predictive Value as a Risk Marker for Subjects and Sites Vulnerable to Bone Loss in a Longitudinal Model of Aggressive Periodontitis. PLoS ONE. 2014;9(6):e98541.
18. 18. Ryu O, Choi S, Linares A, Song I, Kim Y, Jang K et al. Gingival Epithelial Cell Expression of Macrophage Inflammatory Protein-1α Induced by Interleukin-1β and Lipopolysaccharide. Journal of Periodontology. 2007;78(8):1627-1634.
19. 19. Emingil G, Atilla G, Baskesen A, Berdeli A. Gingival crevicular fluid EMAP-II, MIP-1alpha and MIP-1beta levels of patients with periodontal disease. Journal of Clinical Periodontology. 2005;32(8):880-885.
20. 20. Politou M, Terpos E, Anagnostopoulos A, Szydlo R, Laffan M, Layton M et al. Role of receptor activator of nuclear factor-kappa B ligand (RANKL), osteoprotegerin and macrophage protein 1-alpha (MIP-1a) in monoclonal gammopathy of undetermined significance (MGUS). British Journal of Haematology. 2004;126(5):686-689.