INVESTIGATION OF THE EFFECTS OF FOOD ADDITIVE SODIUM BENZOATE ON OXIDATIVE STRESS AND GENOTOXICITY

Year 2023, , 276 - 283, 23.09.2023

İlter İlhan , Duygu Doguc , Muhammet Yusuf Tepebaşı , Okan Sancer , Halil İbrahim Büyükbayram

https://doi.org/10.17343/sdutfd.1091801

Abstract

Objective
Recently, there has been a significant increase in the
use of food additives. Generation of possible side
effects depending on the long-term consumption
of these substances from birth to death has gained
importance in humans. In our study, we aimed to
evaluate the exposure to sodium benzoate in terms
of oxidative stress and genotoxicity, especially in
prepubertal rats.
Material and Method
In our study, 42 male rats (4 weeks-old) divided into
three groups as the control (n=14), experiment 1
(sodium benzoate Acceptable Daily Intake (ADI) dose,
n=14) and experiment 2 (sodium benzoate No Advers
Effect Level (NOAEL)dose, n=14) groups and sodium
benzoate was administration by oral gavage for 6
weeks. At the end of the study, rats were sacrified and
biochemical parameters (glucose, creatinine, AST,
ALT, ALP, lipid profile, total protein, albumin), total
oxidant status (TOS), total antioxidant status (TAS),
ischemia modified albumin (IMA) were measured,
and DNA damage was evaluated by Comet analysis.
Oxidative stress index (OSI) was calculated from the
TOS and TAS parameters.
Results
ALT and creatinine levels were increased with the
NOAEL dosage of sodium benzoate. In addition, TOS,
IMA and OSI levels were elevated, while TAS levels
were decreased in the NOAEL group. Also, NOAEL
dose of sodium benzoate significantly caused DNA
damage.
Conclusions
In our study, no adverse effect was observed in the
administration of sodium benzoate at the dose of
ADI in prepubertal rats. On the other hand, we found
that NOAEL dose of sodium benzoate impairs kidney
and liver functions and causes oxidative stress and
genotoxicity.

Keywords

Comet, Food additive, Oxidative stress, Sodium benzoate

GIDA KATKI MADDESİ SODYUM BENZOATIN OKSİDATİF STRES VE GENOTOKSİSİTE ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI

Abstract

Amaç
Son yıllarda gıda katkı maddelerinin kullanımında belirgin
artış vardır. Doğumdan ölüme kadar maruz kalınan
bu maddelerin, insanlarda uzun süreli tüketime
bağlı olarak oluşturabileceği yan etkiler oldukça önem
kazanmıştır. Çalışmamızda özellikle puberte öncesi
sıçanlarda sodyum benzoata maruziyetin oksidatif
stres ve genotoksisite açılarından değerlendirilmesini
amaçladık.
Gereç ve Yöntem
Kırk iki adet, 4 haftalık erkek sıçan, kontrol (n=14),
deney 1 (Acceptable Daily Intake (ADI) dozunda sodyum
benzoat, n=14) ve deney 2 (No Advers Effect Level
(NOAEL) dozunda sodyum benzoat, n=14) grupları
şeklinde 3 gruba ayrılmıştır. Altı hafta süresince
oral gavaj uygulanmıştır. Çalışma sonunda sakrifiye
edilen sıçanların kan örneklerinden biyokimyasal parametreler
(glukoz, kreatinin, AST, ALT, ALP, lipid profili,
total protein, albümin), total oksidan status (TOS),
total antioksidan status (TAS), iskemi modifiye albümin
(İMA) ölçülmüş ve Comet yöntemi ile DNA hasarı
değerlendirilmiştir. Ölçülen TOS ve TAS parametrelerinden
oksidatif stres indeksi (OSİ) hesaplanmıştır.
Bulgular
NOAEL dozu sodyum benzoat uygulanan sıçanlarda
ALT ve kreatinin seviyeleri yükselmiştir. Ayrıca TOS,
IMA ve OSİ seviyeleri NOAEL grubunda yüksek iken
TAS seviyeleri düşük bulunmuştur. Bunlara ek olarak
NOAEL dozunda sodyum benzoat DNA hasarına yol
açmıştır.
Sonuç
Çalışmamızda, prepubertal dönem sıçanlarda ADI
dozunda sodyum benzoat uygulamasının, olumsuz
bir etkisi gözlemlenmemiştir. Diğer taraftan NOAEL
dozunda sodyum benzoat uygulamasının böbrek ve
karaciğer fonksiyonlarını bozduğunu ve oksidatif stres
ile genotoksisiteye yol açabileceği ortaya konmuştur.

Keywords

Comet, Gıda katkı maddesi, Oksidatif stres, Sodyum benzoat

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