ANKAFERD BLOOD STOPPER'IN KADMİYUMA BAĞLI GELİŞEN AKUT BÖBREK HASARINA ETKİSİ

Öz

Amaç Kadmiyum (Kd) insan sağlığı üzerinde zararlı etkilere neden olabilen çok toksik ve kanserojen bir ağır metaldir. Kd maruziyetine bağlı olarak özellikle böbreklerde toksisite gelişebilir. Ankaferd blood stopper (ABS), cerrahide hemostatik özelliği nedeniyle kullanılan bitkisel bir karışımdır. Ayrıca ABS’nin yara ve doku iyileşmesini artırdığı gösterilmiştir. Bu çalışmada, ABS'nin Kd kaynaklı böbrek hasarına karşı olası koruyucu etkilerini değerlendirmeyi amaçladık. Gereç ve Yöntem Otuz iki erkek sıçan rastgele 4 gruba ayrıldı: kontrol, Kd (kadmiyum klorür, 2,5 mg/kg tek doz ip), ABS (ABS, 1,5 ml/kg tek doz ip) ve Kd+ABS (kadmiyum klorür, 2,5 mg/kg tek doz ip-ABS, 1,5 ml/kg tek doz ip). Deney sonunda sıçanların serumlarından üre ve kreatinin seviyeleri ölçüldü. Ayrıca böbrek dokularından spektrofotometrik olarak total oksidan status (TOS), total antioksidan status (TAS) seviyeleri, süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GPx) enzim aktiviteleri ölçüldü. TOS ve TAS düzeylerinden oksidatif stres indeksi (OSI) hesaplandı. Ayrıca sıçanların böbrek dokusunda Bcl-2-associated X protein (Bax), B-cell-lymphoma-2 (Bcl-2), silenced information regulator 1 (SIRT1) ve p53'ün mRNA ekspresyonundaki değişiklikler qRT-PCR yöntemi ile değerlendirildi. Bulgular Kadmiyum grubunda serum üre, kreatinin düzeyleri ve doku oksidatif stres belirteçleri, TOS ve OSI değerleri kontrol grubuna göre anlamlı olarak yüksek (p<0.05), Gpx aktivitesi anlamlı olarak düşüktü (p<0.05). Ayrıca p53 ekspresyonu ve Bax/Bcl2 oranı Kd grubunda anlamlı artış göstermiştir (p<0.05). Ancak ABS tedavisi, Kd uygulanan grupta üre, kreatinin, TOS, OSI düzeylerini ve Bax/Bcl2 oranı ile p53 ekspresyonunu anlamlı düzeyde azaltmıştır (p<0.05). Sonuç Ankaferd blood stopper akut Kd maruziyetinde oksidatif stresi ve mitokondri aracılı apoptozu azaltarak koruyucu etkiler göstermiştir.

Anahtar Kelimeler

• Urtica dioica
• resveratrol
• meyan
• kekik
• oksidatif stress

PROTECTIVE ROLE OF ANKAFERD BLOOD STOPPER ON CADMIUM-INDUCED ACUTE NEPHROTOXICITY

Öz

Objective Cadmium (Cd) is a very toxic and carcinogenic heavy metal that can cause harmful effects on human health. Toxicity may develop due to Cd exposure, especially in the kidneys. Ankaferd blood stopper (ABS) is a herbal mix that is used for its hemostatic properties in surgery. Also, ABS enhances wound and tissue healing. In this study, we aimed to evaluate the possible ameliorative effects of ABS in Cd-induced renal damage. Material and Method Thirty-two male rats were randomly divided into 4 groups: control, Cd (cadmium chloride, 2.5 mg/kg single dose, ip), ABS (ABS, 1.5 ml/kg single dose ip), and Cd+ABS (cadmium chloride, 2.5 mg/kg single dose ip- ABS, 1.5 ml/kg single dose ip). At the end of the experiment, urea and creatinine levels were analyzed from the rats’ serum. In addition, total oxidant status (TOS), total antioxidant status (TAS) levels, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activity were measured spectrophotometrically from renal tissues. The oxidative stress index (OSI) was calculated from TOS and TAS levels. Also, we evaluated alterations in the mRNA expression of Bcl- 2-associated X protein (Bax), B-cell-lymphoma-2 (Bcl- 2), silenced information regulator 1 (SIRT1), and p53 in kidney tissue of rats by using the qRT-PCR method. Results In the Cd group, serum urea, creatinine levels, and tissue oxidative stress markers, TOS and OSI were significantly higher while Gpx activity was significantly lower than in the control group (p<0.05). Also, the expression of p53 and in Bax/Bcl2 ratio significantly increased in the Cd group (p<0.05). But, ABS treatment significantly decreased urea, creatinine, TOS, OSI levels, and Bax/Bcl2 ratio, p53 expression in Cd applied group (p<0.05). Conclusion Ankaferd blood stopper showed protective effects by reducing oxidative stress and mitochondria-mediated apoptosis in acute Cd exposure.

Anahtar Kelimeler

• Urtica dioica
• oxidative stress
• resveratrol
• licorice
• thyme

Kaynakça

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