Lipid peroxidation and apoptosis levels in gastric tissue of spontaneously hypertensive rats

Öz

Lipid peroxidation and apoptosis levels in gastric tissue of spontaneously hypertensive ratsAim: The spontaneously hypertensive rat is a widely used animal model for essential hypertension . Despitethe recognized gastric complications of hypertension, such as bleeding from gastritis, gastric mucosal bloodflow and motility and vascular ectasias until now no investigations have been conducted on gastric mucosalapoptosis in hypertensive animals. In this study, we investigated the lipid peroxidation and apoptoticchanges in the stomachs on spontaneously hypertensive rats. Methods: Spontaneously hypertensive (n: 10)and normotensive Wistar Kyoto rats (n: 10) were used. Thiobarbituric acid reactive substances weredetermined as an indicator of lipid peroxidation and terminal deoxynucleotdyl transferase-mediateddeoxyuridine triphosphate nick-end labelling technique was used to detect apoptosis in gastric tissue. Results:While the number of apoptotic cells were increased significantly in spontaneously hypertensive rats,thiobarbituric acid reactive substances were not changed when compared Wistar Kyoto rats. In conclusion,data presented in the current study demonstrate the significant increase in gastric mucosal apoptosis inspontaneously hypertensive rats for the first time but underlying mechanisms of apoptosis are not clarified

Anahtar Kelimeler

• Spontaneously hypertansive rat
• apoptosis
• gastric tissue
• lipid peroxidation

Lipid peroxidation and apoptosis in gastric tissue of spontaneously hypertensive rats

Öz

Amaç: Spontan hipertansif sýçanlar esansiyel hipertansiyon için yaygýn olarak kullanýlan bir hayvan modelidir. Hipertansiyonun mide üzerindeki, gastrite baðlý kanama, mide mukozal kan akýmý ve motilite deðiþiklikleri ile damar geniþlemesi gibi çeþitli komplikasyonlarýnýn bilinmesine karþýn, þimdiye kadar hipertansif hayvanlarýn mide mukozal apopitozisiyle ilgili araþtýrma yapýlmamýþtýr. Bu çalýþmada, spontan hipertansif sýçanlarýn midelerinde lipid peroksidasyon düzeyleri ve apopitotik deðiþiklikler araþtýrýldý. Gereç ve Yöntem: Spontan hipertansif (n:10) ve normotansif Wistar Kyoto (n:10) ratlar kullanýldý. Mide mukozasýnda apopitozisin tespiti için terminal deoxynucleotdyl transferase-mediated deoxyuridine triphosphate nick-end labelling
tekniði, lipid peroksidasyonunun göstergesi olarak thiobarbituric acid reactive substances düzeyleri ölçüldü. Bulgular: Spontan hipertansif sýçanlarda apopitotik hücre sayýsýnda Wistar Kyoto sýçanlarla karþýlaþtýrýldýðýnda anlamlý bir artýþ saptanýrken thiobarbituric acid reactive substances düzeyleri deðiþmemiþtir. Sonuç: Sonuç olarak, bu çalýþma ile ilk kez hipertansif sýçanlarýn mide mukozasýnda apopitozisde bir artýþ gösterilmiþtir ancak apopitozise yol açan mekanizmalar kesin deðildir

Anahtar Kelimeler

• Spontan hipertansif sýçan
• apopitozis
• mide dokusu
• lipid peroksidasyon

Kaynakça

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