Association between MDR 1 (ABCB1) gene C3435T, C1236T, G2677T/A, A2956G polymorphisms and the risk of breast cancer among Turkish Women

Year 2020, , 345 - 352, 01.09.2020

Fadime Mutlu İçduygu , Hale Şamlı , Türkkan Evrensel Asuman Özgöz , Kuyaş Hekimler Öztürk Mustafa Canhoroz Adem Deligönül , Necat İmirzalıoğlu

https://doi.org/10.17343/sdutfd.650471

Abstract

Objective: The most
common malignancy in women is breast cancer worldwide. Previous studies
performed in different populations have suggested an association between
Multi-Drug Resistance (MDR1) gene polymorphisms and breast cancer risk in
women. The purpose of the current study is to examine relationship between MDR1
polymorphisms (C3435T, G2677T/A, C1236T, A2956G) and the risk of breast cancer
in Turkish women.

Methods: In this study
35 breast cancer cases and 20 healthy controls were enrolled. Identification
of MDR1 genotypes was performed with the polymerase chain reaction/restriction
fragment length polymorphism (PCR-RFLP) technique.

Results: We observed
significant difference in distribution of C3435T genotypes between the cases
and the controls (cases, CC 37.1%, CT 28.6%, and TT 34.3%; controls, CC 25%, CT
65%, and TT 10%, p: 0.023). On the other hand, no significant differences in
genotype and allele frequencies of C1236T and G2677T/A polymorphisms were
observed between groups. We also found that all subjects carry AA genotype for
A2956G polymorphism.

Conclusion: Although our study group is small, the results suggest that the MDR1
C3435T polymorphism may increase the breast cancer risk in Turkish women.

Keywords

Breast cancer, MDR1 gene, Polymorphism

Supporting Institution

Research Projects Committee of Afyon Kocatepe University

Project Number

Project Number: BAP08.TIP.13

Türk kadınlarında MDR1 (ABCB1) geni C3435T, C1236T, G2677T/A, A2956G polimorfizmleri ile meme kanseri riski arasındaki ilişki

Abstract

Amaç:
Meme kanseri dünya genelinde kadınlarda en yaygın rastlanan kanser türüdür.
Farklı populasyonlarda yapılan daha önceki çalışmalarda MDR1 geni polimorfizmleri
ile kadınlarda meme kanseri riski arasında ilişki olduğu öne sürülmüştür.
Mevcut çalışmanın amacı Türk kadınlarında MDR1 geni C3435T, G2677A/T, C1236T,
A2956G polimorfizmleri ile meme kanseri riski arasındaki ilişkiyi
araştırmaktır.

Yöntem: Bu
çalışmaya 35 meme kanseri hastası ve 20 sağlıklı kontrol dahil edilmiştir. MDR1
genotiplerinin belirlenmesi amacıyla polimeraz zincir reaksiyonu/restriksiyon
uzunluk polimorfizmi (PCR/RFLP) yöntemi kullanılmıştır.

Bulgular: Olgu
ve kontrol grubu arasında C3435T polimorfizminin genotipleri açısından anlamlı
bir fark gözlenmiştir (olgu grubu, CC %37.1, CT %28.6 ve TT %34.3; kontrol
grubu CC %25, CT %65, TT %10, p: 0.023). Diğer taraftan, C1236T ve G2677A/T
polimorfizmlerinin genotip ve allel sıklıkları gruplar arasında farklı
bulunmamıştır. A2956G polimorfizmi için ise tüm çalışma grubunun AA genotipini
taşıdığı belirlenmiştir.

Sonuç:
Çalışmamızda değerlendirilen hasta sayısı az olmakla birlikte, elde ettiğimiz
veriler MDR1 geni C3435T polimorfizminin Türk kadınlarında meme kanseri riskini
artırabileceğini düşündürmektedir. 

Keywords

Meme kanseri, MDR1 geni, Polimorfizm

Project Number

Project Number: BAP08.TIP.13

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