Determination of Relationship between Streptomycin Sülfate Susceptibility and Their Fatty Acid Methyl Esters Profiles of Pseudomonas syringae pv. syringae Strains Isolated from Pome Fruits in East Anatolian Region

Öz

In this study; a total of 20 Pseudomonas syringae pv. syringae strains, isolated from pome fruits, were tested for their susceptibilities to streptomycin sulfate. In addition; the relationship between streptomycin susceptibility of strains and their fatty acid methyl ester (FAME) profiles was determined. According to these results, only one strain was determined to be resistant to streptomycin (5.0%). Four different FAMEs groups of tested strains were determined by using MIS system (Microbial Identification System). Difference between groups was changed from 16% to 60%. The highest rate of resistance (5%) was in second group. Difference of this group from the others was that 12:0 2OH (% 6.60), 12:0 3OH (% 5.59) and 17:0 cyclo (% 11.43) fatty acids were higher than the others. Consequently, we think that fatty acid methyl esters of bacterial strains can be used as an indicator of antibiotic resistance. But, this relationship should be also tested for more Pseudomonas syringae pv. syringae strains and different pathogen species.

Anahtar Kelimeler

• Antibiotic, FAME, fatty acid, MIS, Pseudomonas syringae pv. syringae, streptomycin sulfate

Doğu Anadolu Bölgesinde Yumuşak Çekirdekli Meyvelerden İzole Edilen Pseudomonas syringae pv. syringae Strainlerinin Streptomisin Sülfata Karşı Duyarlılıkları ile Yağ Asidi Metil Esterleri Arasındaki İlişkinin Belirlenmesi

Öz

Bu çalışmada; yumuşak çekirdekli meyvelerden izole edilen toplam 20 Pseudomonas syringae pv. syringae straininin streptomisin sülfata karşı duyarlılıkları test edilmiş, strainlerin yağ asidi metil ester (FAME) grupları da belirlenerek patojenin yağ asitleri ve streptomisin sülfata duyarlılıkları arasındaki ilişki araştırılmıştır. Elde edilen sonuçlara göre sadece 1 strainde (% 5.0) dayanıklılık tespit edilmiştir. MIS (Mikrobiyal Tanı Sistemi) kullanılarak belirlenen yağ asidi metil esterleri bakımından ise bu patojenin 4 farklı FAME grubunun olduğu belirlenmiştir. Gruplar arasındaki farklılığın % 16 ile % 60 arasında değiştiği, dayanıklılık oranının ise 2. grupta en yüksek düzeyde olduğu tespit edilmiştir. 12:0 2OH (% 6.60), 12:0 3OH (% 5.59) ve 17:0 cyclo (% 11.43) yağ asitleri 2. grupta diğer gruplara oranla daha yüksek oranda belirlenmiştir. Sonuç olarak; yağ asidi metil esterlerinin bakterilerde antibiyotiklere duyarlılığın da bir göstergesi olarak kullanılabileceği düşünülmektedir. Ancak, daha fazla Pseudomonas syringae pv. syringae strainlerinde ve farklı tür patojenlerde de benzer çalışmaların yapılıp sonuçların değerlendirilmesi gerekmektedir.

Anahtar Kelimeler

• Antibiyotik, FAME, yağ asidi, MIS, Pseudomonas syringae pv. syringae, streptomisin sülfat

Kaynakça

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