Effects of Kaempferol on Growth and Toxin Production of Head Blight Agent Fusarium culmorum

Yıl 2017, Cilt: 12 Sayı: 1, 24 - 33, 30.06.2017

Özlem Sefer Emre Yörük , Elif Sedef Develi İşıl Melis Zümrüt Ayşe Server Sezer Zeynep Konukçu

Öz

Fusarium species cause root rot and head blight diseases on wheat and barley. In this study, quantitative spore production, expression of tri4 gene which is one of genes responsible for DON production, and qualitative DON production were examined in F. culmorum F24 isolate, agent of head blight. It was determined that spore production was decreased by increasing concentrations of kaempferol (0, 10 ve 20 µg mL-1 kaempferol) and these differences were significant (p<0.001). Total RNA molecules isolated from samples subjected or non-subjected to kaempferol were used in Reverse Transcription Polymerase Chain Reaction (RT-PCR) in order to examine the tri4 expression after they were converted to cDNA. While gene expression was determined in control group, it was not determined in experimental groups. Similarly, spot of 0.27 Rf value was detected on control group on thin layer chromatography, but no spot belonging to DON was detected on experimental groups. Findings obtained from this study showed that kaempferol even at low concentrations decreased the production of spores and repressed the biosynthesis of DON in F. culmorum species. Results of the study is original since that the antifungal effects of kaempferol on this species was not investigated before; output data have importance in terms of plant pathology research.

Anahtar Kelimeler

Antifungal, head blight, deoxynivalenol, Fusarium, kaempferol

Kaempferol’ün Başak Yanıklığı Etmeni Fusarium culmorum’un Üremesi ve Toksin Üretimi Üzerine Olan Etkileri

Öz

Fusarium türleri buğday ve arpada, kök çürüklüğü ve
başak yanıklığı hastalıklarına neden olmaktadır. Bu çalışmada kaempferol uygulaması sonucunda başak yanıklığı
etmeni F. culmorum F24 izolatında niceliksel
olarak spor üretimi, DON üretiminde sorumlu genlerden tri4 geninin anlatımı ve kalitatif olarak DON üretimi
incelenmiştir. Spor üretiminin artan kaempferol konsantrasyonlarında (0, 10 ve
20 µg mL-1 kaempferol)
düştüğü ve bu farkın anlamlı olduğu (p<0.001) saptanmıştır. Kaempferol
uygulanmış ve uygulanmamış örneklerden izole edilen total RNA molekülleri
cDNA’ya çevrildikten sonra Ters Transkripsiyon Polimeraz Zincir Reaksiyonu’nda (RT-PZR)
tri4 anlatımının incelenmesinde kullanılmıştır.
Kontrol grubunda gen anlatımı belirlenirken, deney gruplarında gen anlatımı
saptanmamıştır. Benzer şekilde ince tabaka kromatografisinde de kontrol
grubunda 0.27 Rf (ing., ‘Retention
factor’) değerine sahip spot görülürken, deney gruplarında DON’a ait spot
saptanmamıştır. Elde edilen veriler kaemferol’ün düşük konsantrasyonlarda bile F. culmorum türünde spor üretimini azalttığını
ve DON üretimini baskılayabildiğini göstermiştir. Elde edilen veriler kaempferol’ün
daha önce bu türde antifungal etkisinin araştırılmaması sebebiyle özgünlük
içermekte; veriler bitki patolojisi araştırmaları açısından önem arz
etmektedir.

Anahtar Kelimeler

Antifungal, başak yanıklığı, deoksinivalenol, Fusarium, kaempferol

Kaynakça

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