EFFECTS OF SEPERATE AND COMBINED TREATMENTS OF DIFFERENT DOSES OF GAMMA RAYS AND EMS ON DURUM WHEAT (Tritium durum Desf.) IN M2 GENERATIONS

Yıl 2005, Cilt: 14 Sayı: 1-2, 41 - 49, 01.06.2005

Cemalettin Çiftçi , Ali Şenay

Öz

              The aim of this research was to determine the separete and combine effects of different doses of gamma rays and EMS concentrations on some characteritics of M₂ plants of durum wheat, cv. Kunduru 1149. The seeds of durum wheat, cv. Kunduru 1149 which were irradiated with 50 Gy, 150 Gy and 250 Gy gamma rays and / or treatedEMS for 6 hours at 30°C in 0.2 % and 0.4 % concentrated. The seeds were not presoaked before, they were treated for 6 hours withEMS. The seeds were washed in flushing tap water for 4 hours, after treatment withEMS.

            According to the results of this research; Emergence rate decreased 0.96-4.83 % in M₂ plants of durum wheat, cv. Kunduru 1149. The chlorophyll mutations and mutation frequency and mutagenic efficiency value were increased after combined treatments when compared to other single gamma rays orEMS treatments. They were highest in the treatment 250 Gy + 0.2%EMS.

 

Anahtar Kelimeler

Mutagen; durum wheat;gamma rays; M2 plants

MAKARNALIK BUĞDAYDA (Triticum durum Desf.) GAMA IŞINI VE EMS’İN FARKLI DOZLARININ AYRI AYRI VE BİRLİKTE UYGULANMASININ M2 BİTKİLERİNDEKİ ETKİLERİ

Öz

          Araştırmanın amacı, farklı gama ışını ve EMS dozlarının ayrı ayrı ve birlikte uygulanmasının Kunduru 1149 makarnalık buğday (Triticum durum Desf.) çeşidinin M₂ bitkilerinin bazı özellikleri üzerindeki etkilerini belirlemektir. Bu amaçla, makarnalık buğday tohumlarına 50 Gy, 150 Gy, 250 Gy gama ışını ve % 0.2, % 0.4 EMS dozları ayrı ayrı ve birlikte uygulanmıştır. EMS uygulaması yapılan tohumlara ön ıslatma yapılmamış ve 6 saatlik uygulama süresi sonunda  4 saat süreyle akan su altında yıkanmıştır.

          Araştırma sonuçlarına göre; Kunduru 1149 makarnalık buğday çeşidinin M₂ bitkilerinin çıkış oranında kontrole göre % 0.96 - 4.83 arasında azalma belirlenmiştir. Klorofil mutasyonu tipleri yönünden sırasıyla albino > viridis > tigrina > xantha tipleri saptanmıştır. M₂ bitkilerindeki klorofil mutasyonları, mutasyon frekansı ve mutagenik verim birleşik uygulamalarda, özellikle 250 Gy + % 0.2 EMS uygulamasında daha yüksek bulunmuştur. 

 

Anahtar Kelimeler

Mutagen; makarnalık buğday; gama ışını; M2 bitkileri

Kaynakça

• Aastveit, K.,1968. Effects of Combinations of Mutagens on Mutation Frequency in Barley. Mutation in Plant Breeding II. Proceedings of a Panel, Vienna (11-15 Sept. 1967). Jointly organized by the IAEA and FAO, p.5-14.
• Ando, A. 1968. Mutation induction in rice by radiation combined with chemical protectants and mutagen. Rice Breeding with Induced Mutations II. IAEA, Vienna Technical Reports Series No. 86, pp. 7-15.
• Anonymous, 1977. Manual Mutation Breeding. Technical Reports Series. No 119, IAEA.Vienna.
• Bhatnagar, S. M. 1991. .Induced variability in kabuli Chickpea (Cicer arietinum L.). Plant Mutation Breeding for Crop Improvement, Proceedings of a symposium, Vienna 18-22 June 1990 jointly organized by IAEA and FAO, p. 455-462.
• Cheng, X. and Gao, M. 1988. Biological and genetic effects of combined treatments of sodium Azide, gamma rays and EMS in barley. Environmental and Experimental Botany, 28(4):281-284.
• Constantin, M.J., Conger, B.V., Chowdhury, J.B. and Ramage, R.T. 1974. Cholorophyll - defıcıent mutants ın barley: Effects of chemical mutagens on irradiated and non irradiated seeds after various periods of presoaking. Polyploidy and Induced Mutations in Plant Breeding IAEA, Vienna, p.53-62.
• Choudhary, D.K. and Kaul, B.L. 1976. Effect of sodium azide on X-rays induced seedling injury, chrosome abberrations and frequency of chlorophyll deficient mutations in barley (Hordeum vulgare L.). Indian Journal experimental Biology, 14 : 607 - 609.
• Chauhan, S. P. and Patra, N. K. 1993. Mutagenic effects of combined and single doses of gamma rays and EMS in opium poppy. Plant Breeding 110: 342 345.
• Doll, H. and Sandfaer, J. 1969. Mutagenic effect of gamma rays, dES, EMS and various combinations of gamma rays and the chemicals. Induced Mutations in Plants, Induced Mutations İn Plant Proceedings of a symposium, Pullman 14-18 July 1969 Jointly organized by IAEA and FAO, p.195-206.
• Favret, E. A. 1963. Genetic effects of single and combined treatment of ionizing radiations and ethyl methanesulphonate on barley seeds. 1.st Int. Barley Genetics Symp. Wageningen, pp. 521-536.
• Gustafsson, A. 1940. The mutation system of the chlorophyll aparatus. Lunds Universitets Arsskrift, n.f. Avd. 2, Vol. 36 (11), 1-40.
• Gaul, H. 1963. Mutationen in der Pflanzenzuchtung. Z. Pflanzenzucht 50:194-207.
• Konzak, C. F. Nilan, R.A., Wagner, J. and Foster R. J. 1965. Efficient Chemical Mutagenesis. Radiation Botany (Supplement) vol 5, 49-69.
• Khalatkar, A.S. and Bhatia, C.A.1974. Synergistic effect of combined treatments of gamma radiation and EMS in barley. Radiation Botany, 15(3):223-229.
• Mehandjiev,A.D. 1991. Application of experimental mutagenesis in soybean. Plant mutation Breeding for Crop Improvement, Proceedings of a Sympoium, Vienna 18-22 June 1990, Jointly Organized by IEAE and FAO, vol.1, pp. 407-412.
• Mihov, M. I. M. and Mehandjiev, A. D. 1991. Investigation of mutatıon variability in lentil (Lens culinaris Medic). Plant Mutation Breeding for Crop Improvement Vol.1, p. 399-405 Proceedings of a symposium, Vienna 18-22 June 1990, Jointly organized by IAEA and FAO.
• Nagl, K. 1968. Mutation experiments in durum wheat. Mutation Plant Breeding, IAEA / FAO Vienna, v.II, p. 293-298.
• Nielen, S., 2004. FAO/IAEA Mutant Variety Database. Mutation Plant Breeding, IAEA / FAO Vienna,
• Peşkircioğlu, H. 1995. Arpa (Hordeum vulgare L.)’ya Uygulanan EMS (Ethyl Methane Sulphonate) ve Gama ışınlarının M1 ve M2 Bitkilerinin Bazı Özellikleri Üzerine Etkileri. A.Ü. Fen Bilimleri Enstitüsü, Doktora Tezi, s. 93.
• Reddy, V.R.K., 1992. Mutagenic parameters in single and combined treatments of gamma rays, EMS and sodium azide in triticale barley and wheat. Advances in Plant Sciences, 5:2, 542-553.
• Reddy, V.R.K. and Suganthi, C.P. 1993. Effect of different ploidy levels on chlorophyll mutations frequency in some cereals. Advances in plant sciences, 6:1, 178-191.
• Singh, R.M., Singh, J., Srivastava, A.N. 1977. Mutagenic effects of gamma rays, EMS, and HA in barley. Barley Genetics Newsletter, v.7:60.
• Ünver, S. 1989. Arpa'da Uygulanan EMS(Ethyl Methane Sulphonate) Dozları, Yıkama Suyu Sıcaklık ve Süresinin M1 ve M2 Bitki Özelliklerine Etkileri. A.Ü. Fen Bilimleri Enstitüsü, Tarla Bitkileri Ana Bilim Dalı, Doktora Tezi, s.132, Ankara.
• Vatsya, B., Sharma, R.P. 1981. Improvement of effectiveness and efficiency of mutagens through combination treatments of gamma rays and ethylmethane sulphonate in barley (Hordeum vulgare L.). Journal of Nuclear Agricultural and Biology, India, v. 10(3), p.65-69.