Effects of the entomopathogenic fungus Beauveria bassiana on different stages of the pine processionary moth (Thaumetopoea wilkinsoni Tams)

Öz

Abstract: In this study, local isolates (from Isparta) of the entomopathogenic fungus Beauveria bassiana (Bals.) Vull. (BMAUM M6-4 and LD.2016) and BMAUM M6-4 were tested on second instar and late instar larvae of the pine processionary moth, respectively, under both laboratory and field conditions. The fungal isolates were tested on the second instar larvae at a concentration of 10⁷ conidia/ml by dipping the pine leaves or spraying directly on larvae. For the late instar larvae under laboratory conditions, the fungal isolates were applied to the soil or spraying directly on the insect before the pupal stage. Under field conditions, larvae were collected with a special trap set up on pine trees and the isolates were applied to  the soil or sprayed directly on the larvae. The second instar larvae were highly susceptible to the fungal isolates and 100% fungal infection was observed, at the latest, 5 days after application. The Beauveria bassiana BMAUM M6-4 isolate was used only on last instar larvae due to the observation of 100% infection on the third day after fungus application. The 100% infection value observed in the late instar larvae under laboratory conditions was determined as 75% and 93% for the direct fungus application to the soil and the insect under field conditions. In conclusion, local isolates of B. bassiana were determined to cause infection on early and late larval stages of the pine processionary moth. More detailed studies on the effectiveness of this isolate are needed under field conditions.

Anahtar Kelimeler

• Biological control,pine processionary moth,entomopathogenic fungus,, Beauveria bassiana

Entomopatojen Fungus Beauveria bassiana’nın Çam Keseböceği (Thaumetopoea wilkinsoni Tams)’nin Farklı Dönemlerine Etkileri

Öz

Bu çalışmada yerel olarak izole edilen entomopatojen fungus Beauveria bassiana (Bals.) Vull. izolatlarının (BMAUM M6-4 ve LD.2016) laboratuvar koşullarında çam keseböceğinin ikinci dönem larvalarına ve BMAUM M6-4 izolatının son dönem larvalarına arazi ve laboratuvar koşullarında etkisi belirlenmiştir. Fungal izolatlar ikinci dönem larvalara 10⁷ konidi/ml konsantrasyonunda çam yapraklarını daldırma ve larvalara direkt püskürtme yöntemleri ile denenmiştir. Son dönem larvalara laboratuvar koşullarında toprak içine fungus uygulaması ve pupa dönemine geçmeden böcek üzerine fungus uygulaması yapılmıştır. Arazi koşullarında çam ağaçlarına kurulan özel tuzaklar sayesinde toplanan son dönem larvalara yine toprak içine ve böcek üzerine entomopatojen fungus sporları uygulamıştır. Çalışma sonucunda, ikinci dönem larvaların entomopatojen fungus izolatlarına karşı oldukça hassas oldukları gözlenmiş ve uygulamadan en geç beş gün sonra %100 fungal enfeksiyonu ile ölüm belirlenmiştir. Beauveria bassiana BMAUM M6-4 izolatı özellikle böcek üzerine fungus uygulamasında üçüncü günde %100 enfeksiyona sebep olduğundan son dönem larvalara sadece bu izolat kullanılmıştır. Son dönem larvaların laboratuvar koşullarında gözlemlenen %100 enfeksiyon değeri, arazi koşullarında toprağa ve böceğe direkt fungus uygulamasında %75 ve %93 olarak belirlenmiştir. Lokal olarak izole edilen B. bassiana izolatı, çam keseböceğinin erken dönem ve son dönem larvalarında enfeksiyona neden olup arazi koşullarında uygulanmasının ümitvar olduğu belirlenmiştir.

Anahtar Kelimeler

• Biyolojik mücadele,çam kese böceği,entomopatojen fungus,,Beauveria bassiana

Kaynakça

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