Effects of the entomopathogenic fungus Beauveria bassiana on different stages of the pine processionary moth (Thaumetopoea wilkinsoni Tams)

Yıl 2018, Cilt: 9 Sayı: 1, 7 - 18, 29.06.2018

Sezer Gök Özlem Güven , İsmail Karaca

https://doi.org/10.31019/tbmd.436218

Cited By: 5

Öz

Abstract: In this study, local isolates (from Isparta) of
the entomopathogenic fungus Beauveria
bassiana (Bals.) Vull. (BMAUM M6-4 and LD.2016) and BMAUM M6-4 were tested on
second instar and late instar larvae of the
pine processionary moth, respectively, under both laboratory and field conditions. The fungal isolates were tested on the second
instar larvae at a concentration of 10⁷ conidia/ml by dipping the
pine leaves or spraying directly on larvae. For the late instar larvae under
laboratory conditions, the fungal isolates were applied to the soil or spraying
directly on the insect before the pupal stage. Under field conditions, larvae
were collected with a special trap set up on pine trees and the isolates were
applied to  the soil or sprayed directly
on the larvae. The second instar larvae
were highly susceptible to the fungal isolates and 100% fungal infection was
observed, at the latest, 5 days after application. The Beauveria bassiana BMAUM M6-4 isolate was used only on last instar
larvae due to the observation of 100% infection on the third day after fungus
application. The 100% infection value observed in the late instar larvae under
laboratory conditions was determined as 75% and 93% for the direct fungus
application to the soil and the insect under field conditions. In conclusion,
local isolates of B. bassiana were
determined to cause infection on early and late larval stages of the pine processionary moth. More detailed studies on the effectiveness
of this isolate are needed under field conditions.

Anahtar Kelimeler

Biological control, pine processionary moth, entomopathogenic fungus, , Beauveria bassiana

Entomopatojen Fungus Beauveria bassiana’nın Çam Keseböceği (Thaumetopoea wilkinsoni Tams)’nin Farklı Dönemlerine Etkileri

Öz

Bu
çalışmada yerel olarak izole edilen entomopatojen fungus Beauveria bassiana (Bals.) Vull. izolatlarının (BMAUM M6-4 ve
LD.2016) laboratuvar koşullarında çam keseböceğinin ikinci dönem larvalarına ve
BMAUM M6-4 izolatının son dönem larvalarına arazi ve laboratuvar koşullarında
etkisi belirlenmiştir. Fungal izolatlar ikinci dönem larvalara 10⁷
konidi/ml konsantrasyonunda çam yapraklarını daldırma ve larvalara direkt
püskürtme yöntemleri ile denenmiştir. Son dönem larvalara laboratuvar
koşullarında toprak içine fungus uygulaması ve pupa dönemine geçmeden böcek
üzerine fungus uygulaması yapılmıştır. Arazi koşullarında çam ağaçlarına
kurulan özel tuzaklar sayesinde toplanan son dönem larvalara yine toprak içine
ve böcek üzerine entomopatojen fungus sporları uygulamıştır. Çalışma sonucunda, ikinci dönem
larvaların entomopatojen fungus izolatlarına karşı oldukça hassas oldukları
gözlenmiş ve uygulamadan en geç beş gün sonra %100 fungal enfeksiyonu ile ölüm
belirlenmiştir. Beauveria bassiana
BMAUM M6-4 izolatı özellikle böcek üzerine fungus uygulamasında üçüncü günde
%100 enfeksiyona sebep olduğundan son dönem larvalara sadece bu izolat
kullanılmıştır. Son dönem larvaların laboratuvar koşullarında gözlemlenen %100
enfeksiyon değeri, arazi koşullarında toprağa ve böceğe direkt fungus
uygulamasında %75 ve %93 olarak belirlenmiştir. Lokal olarak izole edilen B. bassiana izolatı, çam keseböceğinin
erken dönem ve son dönem larvalarında enfeksiyona neden olup arazi koşullarında
uygulanmasının ümitvar olduğu belirlenmiştir.

Anahtar Kelimeler

Biyolojik mücadele, çam kese böceği, entomopatojen fungus, , Beauveria bassiana

Kaynakça

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