Determination of the Efficiency of Different Inoculation Methods for the Biological Control of Tomato Damping-Off Caused by Rhizoctonia solani and Pythium deliense

Öz

Abstract: Rhizobacterial isolates can be applied with different inoculation methods in studies of the biological control of plant disease agents. These methods include mixing the suspension of bacteria with autoclaved soil, dipping the roots of seedlings in a bacterial suspension during transplanting or coating seeds with a bacterial suspension before sowing. In this study, the effect of the biocontrol agent Pseudomonads putida 10fp, which was isolated from a tomato rhizosphere, was evaluated for its biocontrol potential against the tomato damping-off disease agents, Rhizoctonia solani and Pythium delicense, under in vivo conditions by using different inoculation methods (application dose and format). There was no difference in the disease incidence recorded for treatments in which P. putida fp10 was applied into the soil, seed or seed+soil and at different doses (108, 109 and 1011 cfu/ml ) against damping-off. However, plant growth was affected at different levels of inoculation. The best growth of both tomato varieties was observed after the application of a bacterial suspension into the soil at 108 cfu/ml .

Anahtar Kelimeler

• Biological control,fluorescent Pseudomonads,Rhizoctonia solani,Pythium deliense,,inoculation methods.

Domateste Rhizoctonia solani ve Pythium deliense Tarafından neden olunan Çökerten Hastalığının Biyolojik Mücadelesinde Farklı İnokulasyon Yöntemlerinin Etkinlikliklerinin Belirlenmesi

Öz

Rizosfer bakterileri ile bitki hastalık etmenlerinin biyolojik mücadelesi üzerine yapılan çalışmalarda kök bakteri izolatları farklı şekillerde uygulanabilmektedirler. Bu metodlar bakteri süspansiyonunun steril toprağa karıştırılması, şaşırtma esnasında fide köklerinin bakteri süspansiyonuna daldırılması veya ekimden önce yüksek sayıdaki bakteri ile tohumların kaplanmasını içermektedir. Bu çalışmada, domates rizosferinden izole edilen ve çökerten zararına yol açan Rhizoctonia solani ve Pythium deliense’ ye karşı in vivo koşullarda etkili olduğu daha önceki çalışmalarla belirlenen Pseudomonas putida 10fp izolatının, bu patojenlere karşı farklı uygulama dozu ve uygulama biçimindeki etkileri araştırılmıştır. Sonuçlar göstermiştir ki P. putida fp10’ nun çökertene karşı tohum, toprak ya da tohum+toprak şeklinde 108, 109 and 1011 cfu/ml gibi farklı konsantrasyon uygulanmaları hastalık şiddetinde farklılık yaratmazken, bitki gelişimini farklı şekillerde etkilemiştir. Her iki domates çeşidinde de en iyi fide gelişimi 108 cfu/ml konsantrasyonunda bakteri süspansiyonunun topraklara yapılan uygulama şeklinde görülmüştür.

Anahtar Kelimeler

• Biyolojik mücadele,floresan Pseudomonas,Rhizoctonia solani,Pythium deliense,, inokulasyon metodları

Kaynakça

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