Efficacy of entomopathogenic nematodes on Myiopardalis pardalina (Bigot, 1891) (Diptera: Tephritidae)

Öz

In this study, the efficacy of four local entomopathogenic nematodes (EPNs), Steinernema affine, Steinernema carpocapsae, Steinernema feltiae and Heterorhabditis bacteriophora, against melon fly (Myiopardalis pardalina), was investigated under laboratory conditions. The tested EPNs were collected from different localities in Turkey and the melon fly pupae were collected from different, infested melon fields of Şükürlü village, Diyarbakır. The efficacy experiments were conducted with 2 treatments, namely 50 and 100 IJs of each species of EPN, and one melon fly pupa in each well of a12-well plate at 25 °C. The mortalities of melon fly pupae, which were recorded 7 days after inoculation with EPNs, ranged from 0.0% to 32.1%, depending on the nematode species. Based on the results of this study, field studies should be conducted to determine the practical effectiveness of the use of H. bacteriophora against the pupae of the melon fly, M. pardalina.  

Anahtar Kelimeler

• Myiopardalis pardalina,enthomopathogen nematod,efficacy

Entomopatojen nematodların Kavun sineği (Myiopardalis pardalina (Bigot, 1891) (Diptera: Tephritidae) üzerindeki etkinliği

Öz

Bu çalışmada kavun sineğinin yerel entomopatojen nematodlara (EPN) olan duyarlılığı laboratuvar koşullarında araştırılmıştır. Çalışmada Steinernema affine, Steinernema carpocapsae, Steinernema feltiae ve Heterorhabditis bacteriophora’ya ait ülkesel EPN izolatları kullanılmıştır. EPN’ler Türkiye’nin farklı illerinden elde edilmiş, kavun sineği pupaları ise Şükürlü Köyü (Çınar-Diyarbakır)’nde zarar gören farklı kavun tarlalarından toplanmıştır. Etkinlik denemeleri 12 kuyucuklu plakalarda (plate), 25 ^oC’de bir kavun sineği pupa/50 ve 100 IJs yoğunluğunda yürütülmüştür. Kavun sineği pupalarındaki ölüm oranları nematod inokulasyonundan 7 gün sonra kayıt edilmiştir. Kavun sineği pupalarında nematod türüne bağlı olarak % 0-32.1 arasında ölüm meydana gelmiştir. Çalışmadan elde edilen sonuçlara dayanarak, H. bacteriophora’nın kavun sineğinin topraktaki pupalarına karşı etkinliğinin doğa çalışmaları ile belirlenmesinin faydalı olacağı düşünülmektedir.

Anahtar Kelimeler

• Myiopardalis pardalina,entomopatojen nematodlar,etkinlik

Kaynakça

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