The Efficacy of Native Entomopathogenic Nematodes against the Pine Processionary Moth, Thaumetopoea pityocampa Den. & Schiff. (Lepidoptera: Thaumetopoeidae)

Öz

The pine processionary moth, Thaumetopoea pityocampa Den. & Schiff. (Lepidoptera: Thaumetopoeidae), is one of the most important pests of forest trees. In this study, the efficacy of native entomopathogenic nematodes (EPNs) against the larvae of T. pityocampa was investigated under laboratory conditions. Isolates of Steinernema carpocapsae Weiser, S. feltiae Filipjev (Rhabditida: Steinernematidae), Heterorhabditis bacteriophora (91) Poinar and H. bacteriophora (200) (Rhabditida: Heterorhabditidae) from İstanbul, Sakarya and Çanakkale Provinces of Turkey were used in the study. Thaumetopoea pityocampa larvae were collected from infested trees on the campus of Canakkale Onsekiz Mart University in Canakkale Province. The efficacy assays were conducted with 1 larva and 200 infective juveniles (IJs) nematodes in a plastic petri dish at 10, 15 and 25 ^oC. The larvae were checked daily for 7 days after inoculation with the IJs and the mortalities were recorded. No mortality of T. pityocampa larvae was observed on the first day. Depending on the nematode species and the temperature, larval mortality ranged from 60% to 100%. Based on the results of this study, there is considerable potential for the use of EPNs for the biological control of T. pityocampa.

Anahtar Kelimeler

• Pine processionary moth,larva,entomopathogenic nematode,efficacy

Yerel Entomopatojen Nematodların Çam Kese Böceği Thaumetopoea pityocampa Den. & Schiff. (Lepidoptera: Thaumetopoeidae)’ya Karşı Etkinliği

Öz

Çam kese böceği, Thaumetopoea pityocampa Den. & Schiff. (Lepidoptera: Thaumetopoeidae) orman ağaçlarının en önemli zararlılarından biridir. Bu çalışmada yerel entomopatojen nematodların (EPN) T. pityocampa larvalarına karşı etkinlikleri laboratuvar koşullarında araştırılmıştır. Çalışmada Steinernema carpocapsae Weiser, S. feltiae Filipjev (Rhabditida: Steinernematidae), Heterorhabditis bacteriophora (91) Poinar ve H. bacteriophora (200) (Rhabditida: Heterorhabditidae) izolatları kullanılmıştır. EPN’ler İstanbul, Sakarya ve Çanakkale illerinden, T. pityocampa larvaları ise Çanakkale Onsekiz Mart Üniversitesi kampüs alanındaki zarar görmüş çam ağaçlarından elde edilmiştir. Etkinlik denemeleri plastik petrilerde 10, 15 ve 25 ^oC’de, 200 IJs/T. pityocampa larva yoğunluğunda yürütülmüştür. Petrilerdeki larvalar nematod inokulasyonundan sonraki 7 gün boyunca kontrol edilmiş ve ölüm oranları kaydedilmiştir. İlk gün T. pityocampa larvalarında ölüm görülmemiştir. Nematod türüne ve sıcaklığa bağlı olarak larvalardaki ölüm oranı %60-100 oranında değişmiştir. Çalışmadan elde edilen sonuçlara göre, T. pityocampa’nın biyolojik mücadelesinde EPN'lerin önemli bir kullanım potansiyeli vardır.

Anahtar Kelimeler

• Pine processionary moth,larva,entomopathogenic nematodes,efficacy

Kaynakça

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