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HetRV6 ve HetPV13 mikovirüslerinin Heterobasidion abietinum ve Heterobasidion annosum izolatlarına aktarımı

Yıl 2020, , 383 - 387, 29.12.2020
https://doi.org/10.18182/tjf.776718

Öz

Heterobasidion annosum Fr. Bref. sensu lato, Kuzey Yarım kürenin ılıman kuşakta yer alan ülkelerinde, özellikle ibreli ağaç türlerinde kök ve kök boğazı çürüklüğüne neden olan oldukça tahripkâr bir hastalık etmenidir. Heterobasidion türlerinin mücadelesinde, fungusun konukçusu olmayan ağaç türlerinin seçimi, kimyasal maddeler ve biyolojik kontrol etmenleri kullanılmaktadır. Ancak, bu kontrol yöntemlerinin hiçbiri patojene karşı tam koruma sağlamamaktadır. Bu bağlamda, hastalık etmeni ile mücadele yöntemlerinin geliştirilmesinde sürdürülebilir alternatif metotların araştırılması, önem arz etmektedir. Heterobasidion türleri de dahil olmak üzere birçok fungal türün, çok sayıda farklı mikovirüs topluluğunu barındırdığı bilinmektedir. Bu mikovirüsler, patojen fungusların neden olduğu kayıpları en aza indirerek hastalığı kontrol altına alabilmek üzere biyokontrol ajanı olarak kullanılmaktadır. Bu çalışmada, mikovirüs aktarımı ile, annosum kök ve alt gövde çürüklüğüne karşı mücadele olanaklarının araştırılması amaçlanmıştır. Denemelerde HetPV3-an1 ve HetRV6-ab6 virüsü ile enfekte iki donör izolat ile virüs içermeyen 5 adet H. annosum sensu str., 20 adet H. abietinum izolatı kullanılmıştır. Her iki grupta yer alan izolatlar %2 malt extract agar bulunan Petri kaplarına karşılıklı olarak ekilmiş ve 20°C’de 3 ay süre ile inkübe edilmiştir. İnkübasyon süresi sonunda, gelişmekte olan hiflerin uç kısmından ve koloniler arasındaki birleşim noktasından alınan hifler, Malt- Orange Serum Agar ortamına aktarılmıştır. Alıcı izolatlara potansiyel dsRNA aktarımının gerçekleşip gerçekleşmediği, spesifik primerler ile RT-PCR metodu kullanılarak belirlenmiştir. Denemeler sonunda, test edilen H. abietinum izolatlarının % 15’ si, H. annosum izolatlarının ise % 20’si dsRNA partikülü başarılı bir şekilde aktarılmış ve RT-PCR ile tek segmentli virüs varlığı kesinleştirilmiştir. Bu sonuçlardan yola çıkılarak, mikrovirüs aktarımı yüzdesinin düşük de olsa başarılı olduğu ve gelecekte in vivo çalışmalarla biyokontrol ajanı olarak kullanılabileceği düşünülmektedir.

Destekleyen Kurum

TÜBİTAK TOVAG, LUKE Ormancılık Araştırma Enstitüsü

Teşekkür

Finlandiya LUKE Ormancılık Araştırma Enstitüsünde çalışan Dr. Eeva Vainio’ya donör izolatları gönderdiği için çok teşekkür ederiz. Ayrıca bu projede kullanılan Heterobasidion izolatları TÜBİTAK TOVAG 104O560 no’lu proje kapsamında elde edilmiştir.

Kaynakça

  • Akıllı, S., Ulubaş-Serçe, Ç., Katırcıoğlu, Y.Z., Maden, S., Rigling, D., 2012. Characterization of hypovirulent isolates of the chestnut blight fungus, Cryphonectria parasitica from the Marmara and Black Sea regions of Turkey. European Journal of Plant Pathology, 135(2): 323-334.
  • Buck, K.W., 1986. Fungal Virology – An Overview. In: Fungal Virology (Ed: Buck, K.W.), CRC Press, Boca Raton, FL, pp. 1-84.
  • Coenen, A., Kevei, F., Hoekstra, R.F., 1997. Factors affecting the spread of doublestranded RNA viruses in Aspergillus nidulans. Genetical Research, 69: 1-10.
  • Cole, T.E., Hong, Y., Brasier, C.M., Buck, K.W., 2000. Detection of an RNA-dependent RNA polymerase in mitochondria from a mitovirus-infected isolate of the Dutch elm disease fungus, Ophiostoma novo-ulmi. Virology, 268: 239-243.
  • Doğmuş Lehtijärvi, H.T., Lehtijärvi, A., Korhonen, K., 2006. Heterobasidion abietinum on Abies species in western Turkey. Forest Pathology, 36: 280-286.
  • Doğmuş Lehtijärvi, H.T., Lehtijärvi, A., Korhonen, K., 2007. Heterobasidion on Abies nordmanniana in northeastern Turkey. Forest Pathology, 37: 387-390.
  • Elliston, J.E., 1982. Hypovirulence. Advanced Plant Pathology, 1: 1-33.
  • Ghabrial, S.A., 1994. New developments in fungal virology. Advanced Virus Research, 43: 303-388.
  • Ghabrial, S.A., 1998. Origin, adaptation and evolutionary pathways of fungal viruses. Virus Genes, 16: 119-131.
  • Ghabrial, S.A., Suzuki. N., 2009. Viruses of plant pathogenic fungi. Annu Review of Phytopathology, 47: 353-384.
  • Heiniger, U., Rigling, D., 1994. Biological control of chestnut blight in Europe. Annual Review of Phytopathology, 32: 581-599.
  • Hodges, C.S., 1969. Modes of infection and spread of Fomes annosus. Annual Review of Phytopathology, 7: 247- 266.
  • Hollings, M., 1982. Mycoviruses and plant pathology. Plant Disease, 66: 1106-1112.
  • Hyder, R., Pennanen, T., Hamberg, L., Vainio, E.J., Piri, T., Hantula, J., 2013. Two viruses of Heterobasidion confer beneficial, cryptic or detrimental effects to their hosts in different situations. Fungal Ecology, 6: 387-396.
  • Ihrmark, K., 2001. Double-stranded RNA elements in the root rot fungus Heterobasidion annosum. PhD Dissertation, Swedish University of Agricultural Sciences, Uppsala, Sweden.
  • Ihrmark, K., Johannesson, H., Stenström, E., Stenlid, J., 2002. Transmission of double-stranded RNA in Heterobasidion annosum. Fungal Genetics and Biology, 36: 147-154.
  • Korhonen, K., Stenlid, J., 1998. Biology of H. annosum. In: Heterobasidion annosum: Biology, Ecology, Impact and Control (Ed: Woodward, S., Stenlid, J., Karjalainen, R., Hüttermann, A.), CAB International, Wallingford, UK, pp. 43-70.
  • Laine, L., 1976. The occurence of Heterobasidion annosum (Fr.) in woody plants in Finland. Communicationes Instituti Forestalis Fenniae, 90(3): 1-52.
  • Lehtijärvi, A., Doğmuş, L.T., Aday, A.G., Oskay, F., 2011. The efficacy of selected biological and chemical control agents against Heterobasidion abietinum on Abies cilicica. Forest Pathology, 41: 470-476.
  • Lemke, P.A., Nash, C.H., 1974. Fungal viruses. Bacteriology Review, 38: 29-56.
  • Magliani, W., Conti, S., Gerloni, M., Bertolotti, D., Polonelli, L., 1997. Yeast killer systems. Clinical Microbiology Reviews, 10: 369-400.
  • McCabe, P.M., Pfeiffer, P., Van Alfen, N.K., 1999. The influence of dsRNA viruses on the biology of plant pathogenic fungi. Trends Microbiology, 7(9): 377-381.
  • Milgroom, M.G., Cortesi, P., 2004. Biological control of chestnut blight with hypovirulence: A critical analysis. Annual Review of Phytopathology, 42: 311-318.
  • Newhouse, J.R., Hoch, H.C., MacDonald, W.L., 1983. The ultrastructure of Endothia parasitica. Comparison of a virulent with a hypovirulent isolate. Canadian Journal of Botany, 61: 389-399.
  • Nibert, M.L., Ghabrial, S.A., Maiss, E., Lesker, T., Vainio, E.J., Jiang, D., Suzuki, N., 2014. Taxonomic reorganization of family partitiviridae and other recent progress in partitivirus research. Virus research, 188: 128-141.
  • Nuss, D.L., Koltin, Y., 1990. Significance of dsRNA genetic elements in plant pathogenic fungi. Annual Review of Phytopathology, 28: 37-58.
  • Steenkamp, E.T., Wingfield, B.D., Swart, W.J., Wingfield, M.J., 1998. Double-stranded RNA and associated virulence in South African isolates of Sphaeropsis sapinea. Canadian Journal of Botany, 76: 1412-1417. Tuomivirta, T.T., Hantula, J., 2003a. Two unrelated double-stranded RNA molecule patterns in Gremmeniella abietina type A code for putative viruses of the families Totiviridae and Partitiviridae. Archive of Virology, 148: 2293-2305.
  • Tuomivirta, T.T., Hantula, J., 2003b. Gremmeniella abietina mitochondrial RNA virus S1 is phylogenically related to the members of the genus Mitovirus. Archive of Virology, 148: 2429-2436.
  • Vainio, E.J., Korhonen, K., Tuomivirta, T.T., Hantula, J., 2010. A novel putative partitivirus of the saprotrophic fungus Heterobasidion ecrustosum infects pathogenic species of the Heterobasidion annosum complex. Fungal Biology, 114: 955-965.
  • Vainio, E.J., Keriö, S., Hantula, J., 2011a. Description of a new putative virus infecting the conifer pathogenic fungus Heterobasidion parviporum with resemblance to Heterobasidion annosum P-type partitivirus. Archives of Virology, 156: 79-86.
  • Vainio, E.J., Hakanpää, J., Dai, Y.C., Hansen, E., Korhonen, K., Hantula, J., 2011b. Species of Heterobasidion host a diverse pool of partitiviruses with global distribution and interspecies transmission. Fungal Biology, 115: 1234 –1243.
  • Vainio, E.J., Hyder, R., Aday, A.G., Hansen, E., Piri, T., Doğmuş-Lehtijärvi, T., 2012. Population structure of a novel putative mycovirus infecting the conifer root-rot fungus Heterobasidion annosum sensu lato. Virology, 422: 366-376.
  • Vainio, E.J., Piri, T., Hantula, J., 2013. Virus community dynamics in the conifer pathogenic fungus Heterobasidion parviporum following an artificial introduction of a partitivirus. Microbial Ecology, 65: 28-38.
  • Vainio, E.J., Müller, M.M., Korhonen, K., Piri, T., Hantula, J., 2015a. Viruses accumulate in aging infection centers of a fungal forest pathogen. The ISME Journal, 9(2): 497-507..
  • Vainio, E.J., Jurvansuu, J., Streng, J., Rajamaki, M.L., Hantula, J., Valkonen, J.P.T., 2015b. Diagnosis and discovery of fungal viruses using deep sequencing of small RNAs. Journal of General Virology, 96(3): 714-725.
  • Vainio, E.J., Hantula, J., 2016. Taxonomy, biogeography and importance of Heterobasidion viruses. Virus Research, 219: 2-10.
  • Vainio, E.J., Hantula, J., 2018. Fungal Viruses. In: Viruses of Microorganisms, (Ed: Hyman, P., Stephen, T.), Caister Academic Press, Abedon, UK, pp. 193–209.
  • Van Diepeningen, A.D., Debets, A.J.M., Hoekstra, R.F., 1998. Intra- and interspecies virus transfer in Aspergilli via protoplast fusion. Fungal Genetics and Biology, 25: 171-180.
  • Van Regenmortel, M.H.V., 2000. Introduction to the Species Concept in Virus Taxonomy. In: Virus Taxonomy: Classification and Nomenclature of Viruses. Seventh Report of the International Committee on Taxonomy of Viruses. (Ed: Van Regenmortel, M.H.V., Fauquet, C.M., Bishop, D.H.L., Carstens, E.B., Estes, M.K., Lemon, S.M., Maniloff, J., Mayo, M.A., McGeoch, D.J., Pringle, C.R., Wicker, R.B.), Academic Pess, New York, USA, pp. 3–16.
  • Varga, J., Kevei, F., Vagvolgyi, C., Vriesema, A., Croft, J.H., 1994. Double-stranded RNA mycoviruses in section Nigri of the Aspergillus genus. Canadian Journal of Microbiology, 40: 325-329.
  • Woodward, S., Stenlid, J., Karjalainen, R., Húttermann, A., 1998. Heterobasidion annosum: Biology, ecology, impact and control. CAB International, Wallingford, UK.

Transmission of HetRV6 ve HetPV13 Mycoviruses to Heterobasidion abietinum and Heterobasidion annosum isolates

Yıl 2020, , 383 - 387, 29.12.2020
https://doi.org/10.18182/tjf.776718

Öz

Heterobasidion annosum Fr. Bref. sensu lato is a highly destructive disease factor that causes root and root collar rot in the temperate zone of the Northern Hemisphere, especially in coniferous tree species. In the control of Heterobasidion species, the selection of tree species that are not host to the fungus, chemical substances and biological control agents are used. However, none of these control methods provide complete protection against the pathogen. In this context, it is important to search for sustainable alternative methods of combating the disease agent. Many fungal species, including Heterobasidion species, are known to host a large number of different mycoviruses. These mycoviruses are used as biocontrol agents to minimize the losses caused by pathogenic fungi and control the disease. In this study, it was aimed to investigate the possibilities of fighting against annosum root and butt rot by mycovirus transmission. In the experiments, two donor isolates infected with HetPV3-an1 and HetRV6-ab6 viruses and 5 H. annosum sensu str. and 20 H. abietinum isolates without virus were used. Isolates in both groups were tested in a dual culture in Petri dishes with 2% malt extract agar and incubated at 20 ° C for 3 months. At the end of the incubation period, the hyphae taken from the tip of the developing hyphae and the junction between the colonies were transferred to Malt-Orange Serum Agar medium. Potential dsRNA transfer to the recipient isolates occurred was determined using the RT-PCR method with specific primers. At the end of the trials, the dsRNA particle of 15% of the tested H. abietinum isolates and 20% of the H. annosum isolates were successfully transferred and the presence of single segment virus was confirmed by RT-PCR. Based on these results, even it is thought that the percentage of mycovirus transmission is low, and it can be used as biocontrol agent in future in vivo studies.

Kaynakça

  • Akıllı, S., Ulubaş-Serçe, Ç., Katırcıoğlu, Y.Z., Maden, S., Rigling, D., 2012. Characterization of hypovirulent isolates of the chestnut blight fungus, Cryphonectria parasitica from the Marmara and Black Sea regions of Turkey. European Journal of Plant Pathology, 135(2): 323-334.
  • Buck, K.W., 1986. Fungal Virology – An Overview. In: Fungal Virology (Ed: Buck, K.W.), CRC Press, Boca Raton, FL, pp. 1-84.
  • Coenen, A., Kevei, F., Hoekstra, R.F., 1997. Factors affecting the spread of doublestranded RNA viruses in Aspergillus nidulans. Genetical Research, 69: 1-10.
  • Cole, T.E., Hong, Y., Brasier, C.M., Buck, K.W., 2000. Detection of an RNA-dependent RNA polymerase in mitochondria from a mitovirus-infected isolate of the Dutch elm disease fungus, Ophiostoma novo-ulmi. Virology, 268: 239-243.
  • Doğmuş Lehtijärvi, H.T., Lehtijärvi, A., Korhonen, K., 2006. Heterobasidion abietinum on Abies species in western Turkey. Forest Pathology, 36: 280-286.
  • Doğmuş Lehtijärvi, H.T., Lehtijärvi, A., Korhonen, K., 2007. Heterobasidion on Abies nordmanniana in northeastern Turkey. Forest Pathology, 37: 387-390.
  • Elliston, J.E., 1982. Hypovirulence. Advanced Plant Pathology, 1: 1-33.
  • Ghabrial, S.A., 1994. New developments in fungal virology. Advanced Virus Research, 43: 303-388.
  • Ghabrial, S.A., 1998. Origin, adaptation and evolutionary pathways of fungal viruses. Virus Genes, 16: 119-131.
  • Ghabrial, S.A., Suzuki. N., 2009. Viruses of plant pathogenic fungi. Annu Review of Phytopathology, 47: 353-384.
  • Heiniger, U., Rigling, D., 1994. Biological control of chestnut blight in Europe. Annual Review of Phytopathology, 32: 581-599.
  • Hodges, C.S., 1969. Modes of infection and spread of Fomes annosus. Annual Review of Phytopathology, 7: 247- 266.
  • Hollings, M., 1982. Mycoviruses and plant pathology. Plant Disease, 66: 1106-1112.
  • Hyder, R., Pennanen, T., Hamberg, L., Vainio, E.J., Piri, T., Hantula, J., 2013. Two viruses of Heterobasidion confer beneficial, cryptic or detrimental effects to their hosts in different situations. Fungal Ecology, 6: 387-396.
  • Ihrmark, K., 2001. Double-stranded RNA elements in the root rot fungus Heterobasidion annosum. PhD Dissertation, Swedish University of Agricultural Sciences, Uppsala, Sweden.
  • Ihrmark, K., Johannesson, H., Stenström, E., Stenlid, J., 2002. Transmission of double-stranded RNA in Heterobasidion annosum. Fungal Genetics and Biology, 36: 147-154.
  • Korhonen, K., Stenlid, J., 1998. Biology of H. annosum. In: Heterobasidion annosum: Biology, Ecology, Impact and Control (Ed: Woodward, S., Stenlid, J., Karjalainen, R., Hüttermann, A.), CAB International, Wallingford, UK, pp. 43-70.
  • Laine, L., 1976. The occurence of Heterobasidion annosum (Fr.) in woody plants in Finland. Communicationes Instituti Forestalis Fenniae, 90(3): 1-52.
  • Lehtijärvi, A., Doğmuş, L.T., Aday, A.G., Oskay, F., 2011. The efficacy of selected biological and chemical control agents against Heterobasidion abietinum on Abies cilicica. Forest Pathology, 41: 470-476.
  • Lemke, P.A., Nash, C.H., 1974. Fungal viruses. Bacteriology Review, 38: 29-56.
  • Magliani, W., Conti, S., Gerloni, M., Bertolotti, D., Polonelli, L., 1997. Yeast killer systems. Clinical Microbiology Reviews, 10: 369-400.
  • McCabe, P.M., Pfeiffer, P., Van Alfen, N.K., 1999. The influence of dsRNA viruses on the biology of plant pathogenic fungi. Trends Microbiology, 7(9): 377-381.
  • Milgroom, M.G., Cortesi, P., 2004. Biological control of chestnut blight with hypovirulence: A critical analysis. Annual Review of Phytopathology, 42: 311-318.
  • Newhouse, J.R., Hoch, H.C., MacDonald, W.L., 1983. The ultrastructure of Endothia parasitica. Comparison of a virulent with a hypovirulent isolate. Canadian Journal of Botany, 61: 389-399.
  • Nibert, M.L., Ghabrial, S.A., Maiss, E., Lesker, T., Vainio, E.J., Jiang, D., Suzuki, N., 2014. Taxonomic reorganization of family partitiviridae and other recent progress in partitivirus research. Virus research, 188: 128-141.
  • Nuss, D.L., Koltin, Y., 1990. Significance of dsRNA genetic elements in plant pathogenic fungi. Annual Review of Phytopathology, 28: 37-58.
  • Steenkamp, E.T., Wingfield, B.D., Swart, W.J., Wingfield, M.J., 1998. Double-stranded RNA and associated virulence in South African isolates of Sphaeropsis sapinea. Canadian Journal of Botany, 76: 1412-1417. Tuomivirta, T.T., Hantula, J., 2003a. Two unrelated double-stranded RNA molecule patterns in Gremmeniella abietina type A code for putative viruses of the families Totiviridae and Partitiviridae. Archive of Virology, 148: 2293-2305.
  • Tuomivirta, T.T., Hantula, J., 2003b. Gremmeniella abietina mitochondrial RNA virus S1 is phylogenically related to the members of the genus Mitovirus. Archive of Virology, 148: 2429-2436.
  • Vainio, E.J., Korhonen, K., Tuomivirta, T.T., Hantula, J., 2010. A novel putative partitivirus of the saprotrophic fungus Heterobasidion ecrustosum infects pathogenic species of the Heterobasidion annosum complex. Fungal Biology, 114: 955-965.
  • Vainio, E.J., Keriö, S., Hantula, J., 2011a. Description of a new putative virus infecting the conifer pathogenic fungus Heterobasidion parviporum with resemblance to Heterobasidion annosum P-type partitivirus. Archives of Virology, 156: 79-86.
  • Vainio, E.J., Hakanpää, J., Dai, Y.C., Hansen, E., Korhonen, K., Hantula, J., 2011b. Species of Heterobasidion host a diverse pool of partitiviruses with global distribution and interspecies transmission. Fungal Biology, 115: 1234 –1243.
  • Vainio, E.J., Hyder, R., Aday, A.G., Hansen, E., Piri, T., Doğmuş-Lehtijärvi, T., 2012. Population structure of a novel putative mycovirus infecting the conifer root-rot fungus Heterobasidion annosum sensu lato. Virology, 422: 366-376.
  • Vainio, E.J., Piri, T., Hantula, J., 2013. Virus community dynamics in the conifer pathogenic fungus Heterobasidion parviporum following an artificial introduction of a partitivirus. Microbial Ecology, 65: 28-38.
  • Vainio, E.J., Müller, M.M., Korhonen, K., Piri, T., Hantula, J., 2015a. Viruses accumulate in aging infection centers of a fungal forest pathogen. The ISME Journal, 9(2): 497-507..
  • Vainio, E.J., Jurvansuu, J., Streng, J., Rajamaki, M.L., Hantula, J., Valkonen, J.P.T., 2015b. Diagnosis and discovery of fungal viruses using deep sequencing of small RNAs. Journal of General Virology, 96(3): 714-725.
  • Vainio, E.J., Hantula, J., 2016. Taxonomy, biogeography and importance of Heterobasidion viruses. Virus Research, 219: 2-10.
  • Vainio, E.J., Hantula, J., 2018. Fungal Viruses. In: Viruses of Microorganisms, (Ed: Hyman, P., Stephen, T.), Caister Academic Press, Abedon, UK, pp. 193–209.
  • Van Diepeningen, A.D., Debets, A.J.M., Hoekstra, R.F., 1998. Intra- and interspecies virus transfer in Aspergilli via protoplast fusion. Fungal Genetics and Biology, 25: 171-180.
  • Van Regenmortel, M.H.V., 2000. Introduction to the Species Concept in Virus Taxonomy. In: Virus Taxonomy: Classification and Nomenclature of Viruses. Seventh Report of the International Committee on Taxonomy of Viruses. (Ed: Van Regenmortel, M.H.V., Fauquet, C.M., Bishop, D.H.L., Carstens, E.B., Estes, M.K., Lemon, S.M., Maniloff, J., Mayo, M.A., McGeoch, D.J., Pringle, C.R., Wicker, R.B.), Academic Pess, New York, USA, pp. 3–16.
  • Varga, J., Kevei, F., Vagvolgyi, C., Vriesema, A., Croft, J.H., 1994. Double-stranded RNA mycoviruses in section Nigri of the Aspergillus genus. Canadian Journal of Microbiology, 40: 325-329.
  • Woodward, S., Stenlid, J., Karjalainen, R., Húttermann, A., 1998. Heterobasidion annosum: Biology, ecology, impact and control. CAB International, Wallingford, UK.
Toplam 41 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Orijinal Araştırma Makalesi
Yazarlar

Ayse Gülden Aday Kaya 0000-0002-5631-6026

Tuğba Doğmuş Bu kişi benim 0000-0002-1050-8823

Yayımlanma Tarihi 29 Aralık 2020
Kabul Tarihi 31 Ekim 2020
Yayımlandığı Sayı Yıl 2020

Kaynak Göster

APA Aday Kaya, A. G., & Doğmuş, T. (2020). HetRV6 ve HetPV13 mikovirüslerinin Heterobasidion abietinum ve Heterobasidion annosum izolatlarına aktarımı. Turkish Journal of Forestry, 21(4), 383-387. https://doi.org/10.18182/tjf.776718
AMA Aday Kaya AG, Doğmuş T. HetRV6 ve HetPV13 mikovirüslerinin Heterobasidion abietinum ve Heterobasidion annosum izolatlarına aktarımı. Turkish Journal of Forestry. Aralık 2020;21(4):383-387. doi:10.18182/tjf.776718
Chicago Aday Kaya, Ayse Gülden, ve Tuğba Doğmuş. “HetRV6 Ve HetPV13 mikovirüslerinin Heterobasidion Abietinum Ve Heterobasidion Annosum izolatlarına aktarımı”. Turkish Journal of Forestry 21, sy. 4 (Aralık 2020): 383-87. https://doi.org/10.18182/tjf.776718.
EndNote Aday Kaya AG, Doğmuş T (01 Aralık 2020) HetRV6 ve HetPV13 mikovirüslerinin Heterobasidion abietinum ve Heterobasidion annosum izolatlarına aktarımı. Turkish Journal of Forestry 21 4 383–387.
IEEE A. G. Aday Kaya ve T. Doğmuş, “HetRV6 ve HetPV13 mikovirüslerinin Heterobasidion abietinum ve Heterobasidion annosum izolatlarına aktarımı”, Turkish Journal of Forestry, c. 21, sy. 4, ss. 383–387, 2020, doi: 10.18182/tjf.776718.
ISNAD Aday Kaya, Ayse Gülden - Doğmuş, Tuğba. “HetRV6 Ve HetPV13 mikovirüslerinin Heterobasidion Abietinum Ve Heterobasidion Annosum izolatlarına aktarımı”. Turkish Journal of Forestry 21/4 (Aralık 2020), 383-387. https://doi.org/10.18182/tjf.776718.
JAMA Aday Kaya AG, Doğmuş T. HetRV6 ve HetPV13 mikovirüslerinin Heterobasidion abietinum ve Heterobasidion annosum izolatlarına aktarımı. Turkish Journal of Forestry. 2020;21:383–387.
MLA Aday Kaya, Ayse Gülden ve Tuğba Doğmuş. “HetRV6 Ve HetPV13 mikovirüslerinin Heterobasidion Abietinum Ve Heterobasidion Annosum izolatlarına aktarımı”. Turkish Journal of Forestry, c. 21, sy. 4, 2020, ss. 383-7, doi:10.18182/tjf.776718.
Vancouver Aday Kaya AG, Doğmuş T. HetRV6 ve HetPV13 mikovirüslerinin Heterobasidion abietinum ve Heterobasidion annosum izolatlarına aktarımı. Turkish Journal of Forestry. 2020;21(4):383-7.