Opopanax hispidus Ekstraktlarının Doğal Diyabet Yönetimi İçin Antioksidan ve Enzim Modülatör Potansiyelinin Araştırılması

Öz

Diabetes mellitus, hiperglisemi ve oksidatif stres ile karakterize edilen, uzun vadeli komplikasyonlara yol açan kronik bir metabolik hastalıktır. Opopanax hispidus (Apiaceae), geleneksel tıpta kullanılan çok yıllık aromatik bir bitki olmasına rağmen, antidiyabetik potansiyeli büyük ölçüde araştırılmamıştır. Bu çalışmada, O. hispidus’un toprak üstü kısımlarından elde edilen sulu ve metanolik ekstraktların antioksidan ve α-glukozidaz inhibitör aktiviteleri değerlendirilmiştir. Fenolik ve flavonoid içerikler HPLC ile analiz edilmiş; kaempferol, rutin, luteolin, klorojenik asit ve p-kumarik asit tanımlanmıştır. Antioksidan aktivite, DPPH ve ABTS radikal süpürme testleri ile belirlenmiş olup, sulu ekstraktın DPPH aktivitesinin (5 mg/mL’de %87.55) metanolik ekstrakta (%65.39) kıyasla daha yüksek olduğu, her iki ekstraktın ise benzer ABTS inhibisyonu (~%87–88) gösterdiği saptanmıştır. İn vitro α-glukozidaz inhibisyonu, doza bağımlı aktivite ortaya koymuş; metanolik ekstrakt 5 mg/mL’de %67.18 inhibisyona ulaşırken, sulu ekstrakt konsantrasyonlar boyunca daha stabil bir inhibisyon göstermiştir (5 mg/mL’de %59.88). Bu bulgular, O. hispidus ekstraktlarının biyolojik olarak aktif fenolik bileşikler açısından zengin olduğunu ve güçlü antioksidan ile orta düzeyde α-glukozidaz inhibitör etkiler sergilediğini göstermekte; bu ekstraktların postprandiyal gliseminin yönetiminde doğal ajanlar olarak potansiyel taşıdığını düşündürmektedir. Moleküler mekanizmaların ve terapötik öneminin daha iyi anlaşılması için ileri in vivo çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

• Opopanax hispidus
• antioksidan
• diabetes mellitus
• fenolik bileşikler

Proje Numarası

0997-YL-24

Exploring the Anti-Oxidative and Enzyme-Modulatory Potential of Opopanax hispidus Extracts for Natural Diabetes Management

Öz

Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia and oxidative stress, contributing to long-term complications. Opopanax hispidus (Apiaceae) is a perennial aromatic plant used in traditional medicine, yet its antidiabetic potential remains largely unexplored. This study evaluated the antioxidant and α-glucosidase inhibitory activities of aqueous and methanolic extracts of O. hispidus aerial parts. Phenolic and flavonoid contents were analyzed by HPLC, identifying kaempferol, rutin, luteolin, chlorogenic acid, and p-coumaric acid. Antioxidant activity was assessed via DPPH and ABTS radical scavenging assays, showing higher DPPH activity for the aqueous extract (87.55% at 5 mg/mL) than the methanolic extract (65.39%), while both exhibited similar ABTS inhibition (~87–88%). In vitro α-glucosidase inhibition revealed dose-dependent activity, with the methanolic extract reaching 67.18% inhibition at 5 mg/mL and the aqueous extract showing more consistent inhibition across concentrations (59.88% at 5 mg/mL). These findings indicate that O. hispidus extracts are rich in bioactive phenolics and display strong antioxidant and moderate α-glucosidase inhibitory effects, suggesting potential as natural agents for managing postprandial glycemia. Further in vivo studies are warranted to clarify the molecular mechanisms and therapeutic relevance of these extracts.

Anahtar Kelimeler

• Opopanax hispidus
• antioxidant
• diabetes mellitus
• phenolic compounds

Destekleyen Kurum

Burdur Mehmet Akif Ersoy University Scientific Research Rrojects Unit

Proje Numarası

0997-YL-24

Teşekkür

This work was supported by the Burdur Mehmet Akif Ersoy University Scientific Research Rrojects Unit [Project number: 0997-YL-24].

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