Effects of diazepam on biotester organisms

Yıl 2018, Cilt: 1 Sayı: 1, 1 - 6, 25.12.2018

Schröder Verginica İancu Irina Mihaela Georgata Pavalache

Öz

Benzodiazepines are the most
prescribed pharmaceuticals as depresses of the nervous system. Among these, the
group of  benzodiazepines with special relevance to diazepam, is the most
extensively studied as potential environmental contaminants. Diazepam has been
detected in WWTP originating from hospitals as well as in effluents from
municipal WWTP plants.

The purpose of the
present study is to identify whether the diazepam solutions generate any
effects at tested specie and from what concentrations these were recorded at
crustaceans organism. The adults of Gammarus pulex
balcanicus (Crustacea Amphipoda) were tested according to GamTox
protocol for aquatic toxicity.

Diazepam injectable
solutions (5 mg/mL) were purchased from Terapia SA.Cluj Napoca, Romania. For
testing, four dilutions were used: 1 µg/mL, 2 µg/mL, 10 µg/mL and 20
µg/mL. The behavior and mortality were evaluated at 12, 24,
36, 48, 72 hours from start.

The lethal effects (30 %) were
presented in the first 12 hours of the exposure at 20 µg/mL at diazepam concentrations. In the next time
more extensive physiology reponses and the specimens mortality were gradually
recorded.  LC50 was recorded at 7, 8 µg/mL, after 36 hours.
The more lethal effects (LC 85 and LC100) were recorded at higher than 10 µg/mL concentrations.

In conclusions, the diazepam
solutions induced changes quickly and more extensive on the physiology of the
bio tester organism, which becoming in 12- 48 hours a toxic substance with
effects on all vital process. These results are representative data about
benzodiazepines effects at aquatic crustacean species, in current context
when the effects of pharmaceuticals as potential
contaminant at these aquatic communities were little  known. 

Anahtar Kelimeler

diazepam, aquatic crustacean, Gammarus

Kaynakça

• 1. Alves S. N., Melo A.L., 2013-Effects of benzodiazepine and ivermectin on Giardia tigrina (Platyhelminthes: Turbelaria), Biosci.J., Uberlandia, 29, 1, 209-215.
• 2. EPA, “Gammarid acute toxicity test,” 850.1020 guideline. EPA 712-C-96-130, 1996, http://www.epa.gov.
• 3. Ferreira A. P., 2014 - Environmental Investigation of Psychiatric Pharmaceuticals: Guandu River, Rio de janeiro State Southeast Brazil, Journal of Chemical Heath Risks, 4 (3), 25-32.
• 4. Gerhardt A., 2011- GamTox: A Low-Cost Multiparametric Ecotoxicity Test with Gammarus ssp. For In and Ex Situ Application. International Journal of Zoology. Doi:10.1155/2011/574536.
• 5. Gomez-Canela C., Miller T.H., Bury N.R., Tauler R., Barron L.P., 2016 – Targeted metabolomics of Gammarus pulex following controlled exposures to selected pharmaceuticals in water, Science of the Total Environment, 562, 777-788.6. http://sitem.herts.ac.uk/
• 7. Miller T.H., Bury N. R., Owen S.F., Barron L.P., 2017 - Uptake, biotransformation and elimination of selected pharmaceuticals in a freshwater invertebrate measured using liquid chromatography tandem mass spectrometry. Chemosphere 183, 389-400.
• 8. Moldovan Z. 2006 – Occurrences of pharmaceutical and personal care products as micropollutants in rivers from Romania, Chemosphere 64, 1808-1817.
• 9. Netherton, M. J., 2011-Uptake and metabolism of pharmaceuticals in aquatic invertebrates, Phd Thesis, University of York.
• 10. Pascoe D., Wanchamai Karntanut, Carsten T Müller, 2003 - Do pharmaceuticals affect freshwater invertebrates? A study with the cnidarian Hydra vulgaris, Chemosphere,Volume 51, Issue 6, Pages 521-528.
• 11. Vânia C., Valdemar I.E., 2009- Psychiatric pharmaceuticals in the enviroment, Chemosphere, 77, 1257-1274.
• 12. Whitacre, David M., 2012 - Reviews of Environmental Contamination and Toxicology, Springer Science& Business Media, vol. 218, 156 p.