Electrochemical determination of antiviral drug Famciclovir in human serum samples at boron-doped diamond electrode

Öz

The electrochemical oxidation of famciclovir was investigated in pH range 2.0-10.0 using differential pulse voltammetry at boron-doped diamond electrode. The experimental results from pH and scan rate studies showed that the oxidation behavior of famciclovir at boron doped diamond electrode was irreversible and diffusion-controlled. Also, by using differential pulse technique at electrode, the anodic peak current is obtained to be linear over the range of concentration 0.5 µM - 12 µM and 6 µM - 100 µM in pH 4.7 acetate buffer solution (ABS) for standard drug solution and human serum, respectively. Limits of detection were 0.022 µM and 0.42 µM for standard drug solution and human serum, respectively. The repeatability, reproducibility, selectivity, precision and accuracy of developed method in all media were investigated and calculated. This method was successfully applied for the analysis of famciclovir human serum samples.

Anahtar Kelimeler

• Famciclovir
• drug analysis
• boron-doped diamond electrode
• human serum samples
• differential pulse voltammetry

Destekleyen Kurum

TÜBİTAK

Proje Numarası

118C526

Teşekkür

Çiğdem Kanbeş Dindar thanks the Scientific and Technological Research Council of Turkey (TUBITAK) under the BIDEB/2218-National Postdoctoral Research Fellowship Program for Turkish Citizens.

Kaynakça

1. [1] Squires KE. An introduction to nucleoside and nucleotide analogues. Antivir Ther 2001;6:1–14.
2. [2] Chen H, Lawler JL, Filman DJ, Hogle JM, Coen DM. Resistance to a nucleoside analog antiviral drug from more rapid extension of drug-containing primers. MBio 2021;12:1–16. https://doi.org/10.1128/mBio.03492-20.
3. [3] Simpson D, Lyseng-Williamson KA. Famciclovir: A review of its use in herpes zoster and genital and orolabial herpes. Drugs 2006;66:2397–416. https://doi.org/10.2165/00003495-200666180-00016.
4. [4] Hodge RAV. Famciclovir and Penciclovir. The Mode of Action of Famciclovir Including Its Conversion to Penciclovir. Antivir Chem Chemother 1993;4:67–84. https://doi.org/10.1177/095632029300400201.
5. [5] Perry CM, Wagstaff AJ. Famciclovir 1995;50:396–415.
6. [6] Uslu B, Ozkan SA. Electroanalytical application of carbon based electrodes to the pharmaceuticals. Anal Lett 2007;40:817–53. https://doi.org/10.1080/00032710701242121.
7. [7] Farghaly OA, Abdel Hameed RS, Abu-Nawwas AAH. Analytical application using modern electrochemical techniques. Int J Electrochem Sci 2014;9:3287–318.
8. [8] Selcuk O, Demir Y, Erkmen C, Yıldırım S, Uslu B. Analytical Methods for Determination of Antiviral Drugs in Different Matrices: Recent Advances and Trends. Crit Rev Anal Chem 2021:1–32. https://doi.org/10.1080/10408347.2021.1908111.

9. [9] Uslu B, Özkan SA, Şentürk Z. Electrooxidation of the antiviral drug valacyclovir and its square-wave and differential pulse voltammetric determination in pharmaceuticals and human biological fluids. Anal Chim Acta 2006;555:341–7. https://doi.org/10.1016/j.aca.2005.09.034.
10. [10] Uslu B, Özkan SA. Anodic voltammetry of abacavir and its determination in pharmaceuticals and biological fluids. Electrochim Acta 2004;49:4321–9. https://doi.org/10.1016/j.electacta.2004.03.041.
11. [11] Altun Y, Dogan-Topal B, Uslu B, Ozkan SA. Anodic behavior of sertindole and its voltammetric determination in pharmaceuticals and human serum using glassy carbon and boron-doped diamond electrodes. Electrochim Acta 2009;54:1893–903. https://doi.org/10.1016/j.electacta.2008.10.010.
12. [12] Ashrafi AM, Gumustas M, Vytřas K, Nematollahi D, Uslu B, Mikysek T, et al. Determination and detailed mechanism study of antiviral drugfosamprenavir using carbon paste electrode in the presenceof Triton X-100. Electrochim Acta 2013;109:381–8. https://doi.org/10.1016/j.electacta.2013.07.100.
13. [13] Uslu B, Topal BD, Ozkan SA. Electroanalytical investigation and determination of pefloxacin in pharmaceuticals and serum at boron-doped diamond and glassy carbon electrodes. Talanta 2008;74:1191–200. https://doi.org/10.1016/j.talanta.2007.08.023.
14. [14] Kul D, Gumustas M, Uslu B, Ozkan SA. Electroanalytical characteristics of antipsychotic drug ziprasidone and its determination in pharmaceuticals and serum samples on solid electrodes. Talanta 2010;82:286–95. https://doi.org/10.1016/j.talanta.2010.04.036.
15. [15] Dogan B, Tuncel S, Uslu B, Özkan SA. Selective electrochemical behavior of highly conductive boron-doped diamond electrodes for fluvastatin sodium oxidation. Diam Relat Mater 2007;16:1695–704. https://doi.org/10.1016/j.diamond.2007.05.001.
16. [16] Topal BD, Özkan SA, Uslu B. Direct electrochemistry of native and denatured alpha-2-Macroglobulin by solid electrodes. J Electroanal Chem 2014;719:14–8. https://doi.org/10.1016/j.jelechem.2014.02.008.
17. [17] Altun Y, Uslu B, Ozkan SA. Electroanalytical characteristics of lercanidipine and its voltammetric determination in pharmaceuticals and human serum on boron-doped diamond electrode. Anal Lett 2010;43:1958–75. https://doi.org/10.1080/00032711003687047.
18. [18] Srinubabu G, Raju CAI, Sarath N, Kumar PK, Rao JVLNS. Development and validation of a HPLC method for the determination of voriconazole in pharmaceutical formulation using an experimental design. Talanta 2007;71:1424–9. https://doi.org/10.1016/j.talanta.2006.04.042.
19. [19] Srinubabu G, Sudharani B, Sridhar L, Seshagiri Rao JVLN. Development and validation of liquid chromatographic and UV derivative spectrophotometric methods for the determination of famciclovir in pharmaceutical dosage forms. Chem Pharm Bull 2006;54:819–22. https://doi.org/10.1248/cpb.54.819.
20. [20] Lotfy HM, El-Moneim Abosen MMA, EL-Bardicy MG. Stability-indicating methods for the determination of famciclovir in the presence of its alkaline-induced degradation product. Drug Test Anal 2010;2:188–99. https://doi.org/10.1002/dta.119.
21. [21] Raman NVVSS, Harikrishna KA, Prasad AVSS, Reddy KR, Ramakrishna K. Development and validation of a stability-indicating RP-LC method for famciclovir. J Pharm Biomed Anal 2009;50:797–802. https://doi.org/10.1016/j.jpba.2009.06.031.
22. [22] Mu P, Xu N, Chai T, Jia Q, Yin Z, Yang S, et al. Simultaneous determination of 14 antiviral drugs and relevant metabolites in chicken muscle by UPLC-MS/MS after QuEChERS preparation. J Chromatogr B Anal Technol Biomed Life Sci 2016;1023–1024:17–23. https://doi.org/10.1016/j.jchromb.2016.04.036.
23. [23] Djerada Z, Feliu C, Tournois C, Vautier D, Binet L, Robinet A, et al. Validation of a fast method for quantitative analysis of elvitegravir, raltegravir, maraviroc, etravirine, tenofovir, boceprevir and 10 other antiretroviral agents in human plasma samples with a new UPLC-MS/MS technology. J Pharm Biomed Anal 2013;86:100–11. https://doi.org/10.1016/j.jpba.2013.08.002.
24. [24] Subrahmanyam K V., Mohanraj P, Saravanan VS, Gopal N. UV spectrophotometric determination of famciclovir. Asian J Chem 2007;19:4911–3.
25. [25] Gaballa AS, Teleb SM, Nour EM. Preparation and spectroscopic studies on charge-transfer complexes of famciclovir drug with different electron acceptors. J Mol Struct 2012;1024:32–9. https://doi.org/10.1016/j.molstruc.2012.04.092.
26. [26] El Gohary NA, Madbouly A, El Nashar RM, Mizaikoff B. Synthesis and application of a molecularly imprinted polymer for the voltammetric determination of famciclovir. Biosens Bioelectron 2015;65:108–14. https://doi.org/10.1016/j.bios.2014.10.024.
27. [27] Rezk MS, El Nashar RM. Dissolution testing and potentiometric determination of famciclovir in pure, dosage forms and biological fluids. Bioelectrochemistry 2013;89:26–33. https://doi.org/10.1016/j.bioelechem.2012.08.006.
28. [28] Ren L, Qi S, Khan IM, Wu S, Duan N, Wang Z. Screening and application of a broad-spectrum aptamer for acyclic guanosine analogues. Anal Bioanal Chem 2021;413:4855–63. https://doi.org/10.1007/s00216-021-03446-w.
29. [29] Karasakal A, ULU ST, Unal DO. Spectrofluorimetric determination of famciclovir in pure and pharmaceutical preparations. Opt Spectrosc (English Transl Opt i Spektrosk 2015;119:318–21. https://doi.org/10.1134/S0030400X1508010X.
30. [30] Famvir_PM. Product monograph PrFAMVIR® (famciclovir) 125mg, 250 mg and 500 mg film-coated. ANTIVIRAL AGENT. Novartis Pharm Canada Inc 2016:1–31. file:///C:/Users/user/Downloads/famvir_scrip_e (1).pdf (accessed April 18, 2022).