A Simple and High Throughput Methodology for Simultaneous Determination of Levodopa and Carbidopa

Öz

Parkinson's disease (PD) is a degenerative disorder of the central nervous system. The motor symptoms of PD disease result from the death of dopamine-generating cells in a region of the mid brain and the dopamine precursor levodopa (L-Dopa) is used for the treatment. Carbidopa (Car) is administered in association with L-Dopa in pharmaceutical formulation as an inhibitor on the decarboxylase activity. Thus, their simultaneous determination is of great importance because of their co-existence in pharmaceutical preparations. Present study deals with a simple method development for simultaneous voltammetric determination of L-Dopa and Car at a pencil graphite electrode (PGE) via monitoring the reduction peak of L-Dopa and the second oxidation peak of Car. The sensitivity of the method was found comparable to other methods depending on the sophisticated electrode modifications and the limits of detection were calculated as sub micromolar levels.

Anahtar Kelimeler

• L-Dopa
• Carbidopa
• pencil graphite electrode
• cyclic voltammetry

Kaynakça

1. Siddiqui MR, Al Othman ZA, Rahman N. Analytical techniques in pharmaceutical analysis: A review. Arabian Journal of Chemistry 2017; 10: 1409–1421. Doi: 10.1016/j.arabjc.2013. 04.016
2. Lee MS and Kerns EH. LC/MS applications in drug development, Mass Spectrometry Reviews, 1999; 18: 187- 279. Doi: 10.1002/(SICI)1098-2787
3. Ge L, Li SP, Lisak G, Advanced sensing technologies of phenolic compounds for pharmaceutical and biomedical analysis. Journal of Pharmaceutical and Biomedical Analysis, 2020; 1795: Article 112913. Doi: 10.1016/j.jpba.2019.112913
4. Özkan SA, Uslu B, Aboul-Enein HY. Analysis of pharmaceuticals and biological fluids using modern electroanalytical techniques. Critical Reviews in Analytical Chemistry, 2003; 33(3):155–181. Doi: 10.1080/713609162
5. Uslu B, Ozkan SA, Electroanalytical Application of Carbon Based Electrodes to the Pharmaceuticals. Anal. Lett. 2006; 40: 817-853. Doi: 10.1080/00032710701242121
6. Gupta VK, Jain R, Radhapyari K, Jadon N, Agarwal, S. Voltammetric Techniques for the Assay of Pharmaceuticals--A Review. Anal. Biochemistry; 2011. 408: 179-196 DOI: 10.1016/j.ab.2010.09.027
7. Antonini A, Yegin A, Preda C, Bergmann L, Poewe W. Global long-term study on motor and non-motor symptoms and safety of levodopa-carbidopa intestinal gel in routine care of advanced Parkinson's disease patients; 12-month interim outcomes. Parkinsonism & Related Disorders 2014. 21 (3):231–35. http://dx.doi.org/10.1016/j.parkreldis. 2014. 12.012
8. Ardakani MM, Khoshroo A. Nano composite system based on coumarin derivative–titanium dioxide nanoparticles and ionic liquid: Determination of levodopa and carbidopa in human serum and pharmaceutical formulations. Analytica Chimica Acta 2013; 798: 25–32. http://dx.doi.org/10.1016/j.aca.2013.08.045

9. Bugamelli F, Marcheselli C, Barba E, Raggi MA. Determination of L-Dopa, carbidopa, 3-O-methyldopa and entacapone in human plasma by HPLC–ED. Journal of Pharmaceutical and Biomedical Analysis, 2011; 54: 562–567. Doi:10.1016/j.jpba.2010.09.042
10. Ozdokur KV, Engin E, Yengin C, Ertas H, Ertas, FN. Determination of Carbidopa, Levodopa, and Droxidopa by High-Performance Liquid Chromatography-Tandem Mass Spectrometry. Analytical Letters, 2017; 51(1-2): 73-82. Doi: 10.1080/00032719.2017.1316283
11. Ardakani M, Taleat Z, Khoshroo A, Beitollahi H, Dehghani H. Electrocatalytic oxidation and voltammetric determination of levodopa in the presence of carbidopa at the surface of a nanostructure based electrochemical sensor. Biosensors and Bioelectronics, 2012; 35 (115) 75-81. Doi:10.1016/j.bios.2012.02.014
12. 1Mohammadi SZ, Beitollahi H, Kaykhaii M, Mohammadizadeh N, Tajik S, Hosseinzadeh R. Simultaneous determination of droxidopa and carbidopa by carbon paste electrode functionalized with NiFe2O4 nanoparticle and 2-(4-ferrocenyl-[1,2,3] triazol-1-yl)-1-(naphthalen-2-yl) ethanone Measurement; 2020; 155: 107522. Doi: 10.1016/j.measurement.2020.107522
13. Quintino MSM, Yamashita M, Angnes L. Voltammetric Studies and Determination of Levodopa and Carbidopa in Pharmaceutical Products. Electroanalysis, 2006; 18: 655-661. Doi: 10.1002/elan.200503445
14. Wang Q, Das MR, Li M, Boukherroub R, Szunerits S. Voltammetric detection of L-Dopa and carbidopa on graphene modified glassy carbon interfaces. Bioelectrochemistry, 2013; 93: 15–22. Doi: 10.1016/j.bioelechem.2012.03.004
15. Zapata-Urzúa C, Pérez-Ortiza M, Bravo M, Olivieri AC, Álvarez-Lueje A. Simultaneous voltammetric determination of levodopa, carbidopa and benserazide in pharmaceuticals using multivariate calibration. Talanta, 2010; 82: 962–968. Doi: 10.1016/j.talanta.2010.05.071
16. Beitollah Hadis, Goodarzian M, Khalilzadeh MA, Karimi-Maleh H, Hassanzadeh M, Tajbakhsh M, Electrochemical behaviors and determination of carbidopa on carbon nanotubes ionic liquid paste electrode. Journal of Molecular Liquids, 2012, 173: 137–143. Doi: 10.1016/j.molliq.2012.06.026
17. David JG, Popa DE, Buleandra M, Pencil Graphite Electrodes: A Versatile Tool in Electroanalysis. Journal of Analytical Methods in Chemistry, 2017;Article ID 1905968. Doi: 10.1155/2017/1905968
18. Dehnavi A, Soleymanpour A. Highly sensitive voltammetric electrode for the trace measurement of methyldopa based on a pencil graphite modified with phosphomolibdate/graphene oxide. Microchemical Journal, 2020; 157: Article 104969. Doi: 10.1016/j.microc.2020.104969
19. Liv L, Nakiboğlu N. Simple and rapid voltammetric determination of boron in water and steel samples using a pencil graphite electrode. Turkish Journal of Chemistry, 2016; 40: 412 – 421. Doi: 10.3906/kim-1507-64
20. Ozsoz M, Erdem A, Kerman K, Ozkan D, Tugrul B, Topcuoglu N, Ekren H, Taylan M. Electrochemical Genosensor Based on Colloidal Gold Nanoparticles for the Detection of Factor V Leiden Mutation Using Disposable Pencil Graphite Electrodes. Analytical Chemistry, 2003; 75 (9), 2181–2187. Doi: 10.1021/ac026212r
21. Ardakani M. M., Ganjipour B., Beitollahi H., Amini M. K., Mirkhalaf F., Naeimi H., Barzoki M. N. Simultaneous determination of levodopa, carbidopa and tryptophan using nanostructured electrochemical sensor based on novel hydroquinone and carbon nanotubes: Application to the analysis of some real samples. Electrochimica Acta, 2011, 56: 9113– 9120. Doi:10.1016/j.electacta.2011.07.021
22. Tajik S., Taher M. A., Beitollahi H. Simultaneous determination of droxidopa and carbidopa using a carbon nanotubes paste electrode. Sensors and Actuators B, 2013, 188: 923– 930. http://dx.doi.org/10.1016/j.snb.2013.07.085
23. Benvidi A., Firouzabadi A. D., Ardakani M. M., Mirjalili B. B. F., Zare R. Electrochemical deposition of gold nanoparticles on reduced graphene oxide modified glassy carbon electrode for simultaneous determination of levodopa, uric acid and folic acid. Journal of Electroanalytical Chemistry, 2015, 736: 22–29. http://dx.doi.org/10.1016/j.jelechem.2014.10.020
24. Rastakhiz N., Beitollahi H., Kariminik A., Karimi F. Voltammetric determination of carbidopa in the presence of uric acid and folic acid using a modified carbon nanotube paste electrode. Journal of Molecular Liquids, 2012, 172: 66–70. Doi:10.1016/j.molliq.2012.04.013