HPLC analyses of polyphenolic compounds in oak (Querces frainetto) honey from Kırklareli region of Turkey

Öz

In this study, phenolic profile and total phenolic contents of oak honeys, a kind of dark honeydew honey, was determined.  The methanolic honey extract was enriched by liquid; liquid extraction with diethyl ether and ethyl acetate, and then was analyzed by RP-HPLC-UV with acetonitrile: water mobile phase. Nineteen phenolic standards were used to prepare calibration graphics. Seven phenolic acid (gallic acid, protocatequic acid, p-OH benzoic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid) and twelve flavanoids (catechin, epicatechin, rutin, myerecetin, resveratrol, daidzein, luteolin, t-cinnamic acid, hesperetin, chrysin, pinocembrin, caffeic acid phenlyester (CAPE) were used.  Total phenolic contents of the honeys were measured by Folic Ciocalteau's assay.  All of the polyphenols except epicatechin, rutin, luteolin and hesperetin were detected in varying amounts of the honey samples. Protocatequic acid, ferulic acid, myricetin and chrysin were the most abundant phenolic compounds. Total phenolic contents of the honey were from 54 to 88 mg GAE/100 g.  In summary, oak honey has a high apitherapeutic value with rich polyphenol diversity.

Anahtar Kelimeler

• Antioxidant,Oak Honey

Kaynakça

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