Haplophyllum suaveolens Varyetelerinin Antioksidan Aktivitesi ve Sekonder Metabolitleri Üzerine Farklı Çözücülerin Etkisi

Yıl 2019, Cilt: 6 Sayı: 3, 277 - 284, 31.10.2019

Cennet Yaman Deniz Ulukuş Osman Tugay

https://doi.org/10.19159/tutad.590387

Cited By: 2

Öz

Bu çalışmada, Haplophyllum
suaveolens varyetelerine ait methanol ve etanol ekstraktlarının toplam
biyoaktif içerikleri ile antioksidan aktivite seviyelerinin belirlenmesi
amaçlanmıştır. Ekstraksiyon işleminde Haplophyllum
suaveolens varyeteleri olan Haplophyllum
suaveolens (DC.) G.Don var. suaveolens
(HS) ve endemik Haplophyllum suaveolens
var. cilicicum (Boiss.) C.C.Townsend
(HC) taksonlarının herba kısımları kullanılmıştır. Toplam fenolik içerik
Folin-Ciocalteu metotuna göre belirlenmiş ve her bir ekstrakttaki miktarları mg
gallik asit eş değer (GAE) g^-1 ekstrakt olarak ifade edilmiştir.
Ekstraktların toplam fenolik içerikleri 39.54-44.16 mg GAE g^-1
ekstrakt arasında değişim göstermiştir. Toplam flavonoid içerikleri ise mg
kuersetin eş değer (QE) g^-1 ekstrakt olarak ölçülmüş ve 17.30-41.40
mg QE g^-1 ekstrakt arasında değişim göstermiştir. Çalışmada, HS ve
HC taksonlarının methanol ekstraktları en yüksek 2,2-difenil-1-pikrilhidrazil
(DPPH, sırayla % 79.76 ve % 75.69) ve 2,2’-azinobis (3-etil-bezotiazolin 6
sulfonat) (ABTS, sırayla % 54.74 ve % 55.13) radikal kovucu aktiviteleri
göstermiştir. Böylece, Haplophyllum
suaveolens varyetelerinin güçlü radikal kovucu aktivite sergilediği ve her
iki varyete içinde metanol ekstraktların etanol ekstraktlarına göre daha etkili
olduğu tespit edilmiştir. Bu çalşma ile Haplophyllum
suaveolens varyetelerinin güçlü radikal kovucu aktiviteye sahip olduğu,
tıbbi ve ticari kullanım için iyi birer doğal antioksidan kaynağı olabileceği
tespit edilmiştir.

Anahtar Kelimeler

Haplophyllum L., Antioksidan aktivitesi, flavonoid, Haplophyllum L., fenolik

Effect of Different Solvents on Antioxidant Activities and Secondery Metabolites of Haplophyllum suaveolens Varieties

Öz

In
this study, total bioactive contents and antioxidant activity levels of
methanol and ethanol extracts of Haplophyllum
suaveolens varieties were determined. Aerial parts of Haplophyllum
suaveolens varieties which are Haplophyllum suaveolens (DC.) G. Don var. suaveolens (HS) and endemic
Haplophyllum suaveolens var.
cilicicum (Boiss.) C.C. Townsend (HC), were used in the extraction
process. Total phenolic content was
determined according to the Folin-Ciocalteu method and the amounts in each
extract were expressed as mg gallic acid equivalent (GAE) g-1 extract. The total phenolic contents of the extracts
varied between 39.54-44.16 mg GAE g^-1 extract. Total flavonoid
contents were measured as mg quercetin equivalent (QE) g^-1 extract
and varied between 17.30-41.40 mg QE g^-1 extract. The methanol
extracts of HS and HC exhibited maximum radical scavenging activities for DPPH
radical (79.76% and 75.69%, respectively) and ABTS (54.74% and 55.13%,
respectively). Thus, Haplophyllum
suaveolens varieties exhibited strong radical scavenging activity and
methanol extracts were found to be more effective than ethanol extracts in both
varieties. In this study, it has been determined that Haplophyllum suaveolens varieties have strong radical scavenging
activity and maybe good natural antioxidant sources for medical and commercial
use.

Anahtar Kelimeler

Haplophyllum L., Antioxidant activity, flavonoid, Haplophyllum L., phenolic

Kaynakça

• Al-Yahya, M.A., Al-Rehaily, A.J., Ahmad, M.S., Al-Said, M.S., El-Feraly, F.S., Hufford, C.D., 1992. New alkaloids from Haplophyllum tuberculatum. Journal of Natural Products, 55(7): 899-903.
• Anonymous, 2001. IUCN Red List Categories: Version 3.1. Prepared by the IUCN Species Survial Commission. IUCN, Gland Switzerland and Cambridge, UK.
• Arvouet-Grand, A., Vennat, B., Pourrat, A., Legret, P., 1994. Standardisation d`un extrait de propolis et identification des principaux constituants. Journal de Pharmacie de Belgique, 49: 462-468.
• Awaad, A.S., Alothman, E.A.A., 2018. Antiulcer and anti-ulcerative colitis activities of Haplophyllum tuberculatum (Forsskal). International Journal of Pharmacology, 14(1): 31-38.
• Bergheul, S., Berkani, A., Saiah F., Djibaoui, R., 2017. Chemical composition and antifungal activity of essential oils of Haplophyllum tuberculatum (Forssk.) A. Juss. from South Algerian. International Journal Bioscience, 10(5): 97-105.
• Chen, Z., Bertin, R., Froldi, G., 2013. EC50 estimation of antioxidant activity in DPPH assay using several statistical programs. Food Chemistry, 138(1): 414-420.
• Dai, J., Mumper, R.J., 2010. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules, 15(10): 7313-7352.
• Debouba, M., Khemakhem, B., Zouari, S., Meskine, A., Gouia, H., 2014. Chemical and biological activities of Haplophyllum tuberculatum organic extracts and essential oil. Journal of Essential Oil Bearing Plants, 17(5): 787-796.
• Do, Q.D., Angkawijaya, A.E., Tran-Nguyen, P.L., Huynh, L.H., Soetaredjo, F.E., Ismadji, S., Ju, Y., 2014. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22(3): 296-302.
• Duarte, A.R., Naves, R.R., Santos, S.C., Seraphinand, J.C., Ferri, P.H., 2010. Genetic and environmental influence on essential oil composition of Eugenia dysenterica. Journal Brazilian Chemical Social, 21(8): 1459-1467.
• Düzgüneş, O., Kesici, T., Gürbüz, F., 1983. İstatistik Metotları 1. Ankara Üniversitesi Ziraat Fakültesi Yayıncılığı, No: 862, Ankara.
• Erşan, S., Güçlü Üstündağ, Ö., Carlea, R., Schweiggert, R.M., 2018. Subcritical water extraction of phenolic and antioxidant constituents from pistachio (Pistacia vera L.) hulls. Food Chemistry, 253(1): 46-54.
• Gezer, K., Duru, M.E., Kıvrak, I., Türkoğlu, A., Mercan, N., Türkoğlu, H., Gülcan, S., 2006. Free-radical scavenging capacity and antimicrobial activity of wild edible mushroom of Turkey. African journal of Biyotechnology, 5(20): 1924-1928.
• Hamdi, A., Majouli, K., Flamini, G., Marzouk, B., Marzouk, Z.V., Heyden, Y., 2017. Antioxidant and anticandidal activities of the Tunisian Haplophyllum tuberculatum (Forssk.) A. Juss. essential oils. South African Journal of Botany, 112: 210-214.
• Hosni, K., Msaada, K., Taârit, B.M., Marzouk, B., 2011. Phenological variations of secondary metabolites from Hypericum triquetrifolium Turra. Biochemical Systematics and Ecology, 39(1): 43-50.
• Koley, T.K., Kaur, C., Nagal, S., Walia, S., Jaggi, S., Sarika, 2016. Antioxidant activity and phenolic content in genotypes of Indian jujube (Zizyphus mauritiana Lamk.). Arabian Journal of Chemistry, 9(2): 1044-1052.
• Li, B., Zhang, C., Peng, L., Liang, Z., Yan, X., Zhu, Y., Liu, Y., 2015. Comparison of essential oil composition and phenolic acid content of selected Salvia species measured by GC–MS and HPLC methods. Industrial Crops Products, 69: 329-334.
• Li, X.C., Lin, J., Han, W.J., Mai, W.Q., Wang, L., Li, Q., Lin, M.F., Bai, M.S., Zhang, L.S., Chen, D.F., 2012. Antioxidant ability and mechanism of Rhizoma Atractylodes macrocephala. Molecules, 17(11): 13457-13472.
• Lu, C.L., Li, Y.M., Fu, G.Q., Yang, L., Jiang, J.G., Zhu, L., Lin, F.L., Chen, J., Lin, Q.S., 2011. Extraction optimisation of daphnoretin from root bark of Wikstroemia indica (L.) C.A. and its anti-tumour activity tests. Food Chemistry, 124(4): 1500-1506.
• Miller, N.J., Rice-Evans, C., Devies, M.J., Gopinathan, V., Milner, A., 1993. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical Science, 84: 407-412.
• Mirjalili, M.H., Salehi, P., Sonboli, A., Vala, M.M., 2006. Essential oil variation of Salvia officinalis aerial parts during its phenological cycle. Chemistry of Natural Compounds, 42(1): 19-23.
• Mohamed, A.H., Ali, M.B., Bashir, A.K., Salih, A.M., 1996. Influence of Haplophyllum tuberculatum on the cardiovascular system. International Journal of Pharmacognosy, 34(3): 213-217.
• Mossa, J.S., Al-Yahya, M.A., Al-Meshal, I.A., 1987. Medical Plants of Saudi Arabia. Vol. 1, Riyadh, King Saud University Libraries.
• Mossa, J.S., Al-Yahya, M.A., Al-Meshal, I.A., 2012. Medical Plants of Saudi Arabia. King Saud University Libraries, Riyadh.
• Parhoodeh, P., Rahmani, M., Hashim, N.M., Sukarı, M.A., Ee, G.C.L., 2012. Alkaloid constituents of Haplophyllum laeviusculum (Rutaceae). Sains Malaysiana, 41(1): 47-52.
• Pavlović, D.R., Zlatković, B., Živanović, S., Kitić, D., Golubović, T., 2018. Serbian Rutaceae species: comparison of chemical profiles and radical scavenging activity. Biologica Nyssana, 9(1): 37-43.
• Prasad, K.N., Kong, K.W., Ramanan, R.N., Azlan, A., Ismail, A., 2012. Determination and optimization of flavonoid and extract yield from brown mango using response surface methodology. Separation Science and Technology, 47(1): 73-80.
• Prieto, J.M., 2012. In Book: Bioactive Compounds: Types, Biological Activities and Health Effects, Chapter: Haplophyllum A. Juss, A Rich Source of Bioactive Natural Principles, Publisher: Nova, 341-380.
• Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C., 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine, 26(9-10): 1231-1237.
• Russo, A., Formisano, C., Rigano, D., Senatore, F., Delfine, S., Cardile, V., Rosselli, S., Bruno, M., 2013. Chemical composition and anticancer activity of essential oils of Mediterranean sage (Salvia officinalis L.) grown in different environmental conditions. Food Chemical Toxicogical, 55: 42-47.
• Sağlam, H., Gözler, T., Gözler, B., 2003. A new prenylated arylnaphthalene lignan from Haplophyllum myrtifolium. Fitoterapia, 74(6): 564-569.
• Said, O., Khalil, K., Fulder, S., Azaizeh, H., 2002. Ethnopharmacological survey of medicinal herbs in Israel, the Golan Heights and the West Bank region. Journal of Ethnopharmacology, 83(3): 251-265.
• Schinella, G.R., Tournier, H.A., Prieto, J.M., Rı́os, J.L., Buschiazzo, H., Zaidenberg, A., 2002. Inhibition of Trypanosoma cruzi growth by medical plant extracts. Fitoterapia, 73(7-8): 569-575.
• Schinella, G.R., Tournier, H.A, Zaidenberg, A., Prieto, J.M., 2008. On the preclinical anti-trypanosomal, anti-inflammatory and toxicological activities of Haplophyllum linifolium (L.) G. Don and its diphyllin derivatives. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 7(5): 225-228.
• Singleton, V.L., Orthofer, R., Lamuela-Raventos, R.M., 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299: 152-178.
• Snedecor, G.W., Cochran, W.G., 1967. Statistical Methods. The Iowa State University.
• Stalikas, C.D., 2007. Extraction, separation, and detection methods for phenolic acids and flavonoids. Journal of Separation Science, 30(18): 3268-3295.
• Sun, T., Ho C.H., 2005. Antioxidant activities of buckwheat extracts. Food Chemistry, 90(4): 743-749.
• Tirzitis, G., Bartosz, G., 2010. Determination of antiradical and antioxidant activity: Basic principles and new insights. Acta Biochimica Polonica, 57(2): 139-142.
• Townsend, C.C., 1967. Haplophyllum A. Juss. In: P.H. Davis (Ed), Flora of Turkey and The Aegean Islands, Edinburgh University Press. Edinburgh, Vol 2, pp. 496-506.
• Townsend, C.C., 1986. Taxonomic revision of the genus Haplophyllum (Rutaceae). In: P.S. Green (Ed.), Hooker’s icones plantarum, Kew Publishing, 40(1): 336.
• Tugay, O., Ulukuş, D., 2017. Haplophyllum sahinii (Rutaceae), a new species from Central Anatolia (Turkey). Phytotaxa, 29(3): 265-272.
• Ulubelen, A., Öztürk, M,, 2008. Alkaloids, coumarins and lignans from Haplophyllum species. Records of Natural Products, 2(3): 54-69.
• Yamauchi, K., Mitsunaga, T., Muddathir, A.M., 2016. Screening for melanogenesis-controlled agents using Sudanese medicinal plants and identification of active compounds in the methanol extract of Terminalia brownii bark. Journal of Wood Science, 62(3): 285-293.
• Zengin, G., Sarikurkcu, C., Aktumsek, A., Ceylan, R., Ceylan, O., 2014. A comprehensive study on phytochemical characterization of Haplophyllum myrtifolium Boiss. endemic to Turkey and its inhibitory potential against key enzymes involved in Alzheimer, skin diseases and type II diabetes. Industrial Crops and Products, 53: 244-251.