AYÇİÇEĞİ BALI TEMELLİ GÜMÜŞ NANOPARTİKÜLLERİN YEŞİL SENTEZİ, KARAKTERİZASYONU VE BİYOLOJİK AKTİVİTELERİNİN BELİRLENMESİ

Yıl 2024, , 311 - 324, 18.11.2024

Merve Keskin , İrem Uysal Gözde Gürcan

https://doi.org/10.31467/uluaricilik.1529043

Öz

Tıp, ilaç salınım sistemleri, eczacılık, tarım gibi geniş bir yelpazede uygulama alanı bulan nanoteknolojinin yapı taşları olan altın, gümüş, çinko gibi nanopartiküller yeşil sentez tekniği kullanılarak çevre dostu, ekonomik ve biyouyumlu olarak sentezlenebilmektedir. Gümüş nanopartiküllerin yeşil sentezinde içermiş oldukları biyoaktif bileşenler nedeniyle bitkiler veya bitki temelli ürünler yaygın olarak kullanılmaktadır. Bal içermiş olduğu fenolik bileşenler ve şekerler ile gümüş nanopartiküllerin sentezinde kullanılabilecek önemli doğal ürünlerden biridir. Yapılan bu çalışmada, biyoaktif bileşen yönünden kestane ve meşe balına göre daha zayıf olan ayçiçeği balının gümüş nanopartiküllerin sentezinde kullanım potansiyeli tespit edilmiştir. Sentezlenen ayçiçeği balı temelli gümüş nanopartiküller (SH-AgNPs) karakterize edilmiş ve daha sonra antioksidan aktiviteleri ile yara iyileşmede önemli rolü olan myeloperoksidaz ve kollegenaz enzimleri inhibe etme özellikleri tespit edilmiştir. Sentezlenen nanopartiküllerin 440 nm’ de maksimum absorbans verdiği, partikül boyutlarnın 33 nm ile 38 nm arasında değiştiği tespit edilmiştir. Sentezlenen nanopartiküllerin DPPH·radikal süpürme aktiviteleri ve FRAP demir indirgeme kapasiteleri sırasıyla % 81±1,42 and % 86±1,24; myleoperksidaz ile kollegenaz enzimlerini inhibe etme özellikleri sırasıyla % 63±1,45 and % 37±1,14 olarak tespit edildi. Elde edilen bulgular ayçiçeği balının nanoteknoloji alanında kullanım potansiyeli olduğunu göstermektedir.

Anahtar Kelimeler

Biyolojik sentez, Gümüş nanopartikül, Nanoteknoloji, Antioksidan aktivite, Enzim inhibisyonu

Destekleyen Kurum

Bu çalışma, TÜBİTAK Bilim İnsanı Destek Programları Başkanlığı (BİDEB) tarafından yürütülen 2209-A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı TÜBİTAK 2209-A tarafından 1919B012208098 proje numarası ile desteklenmiştir.

Proje Numarası

1919B012208098 (TÜBİTAK 2209-A)

Green Synthesis, Characterization and Determination of Biological Activity of Sunflower Honey Based-Silver Nanoparticles

Öz

Nanoparticles such as gold, silver, zinc, which are the building blocks of nanotechnology, which have a wide range of applications in medicine, drug release systems, pharmacy, agriculture, can be synthesized in an environmentally friendly, economical and biocompatible way using green synthesis technique. In the green synthesis of silver nanoparticles, plants or plant-based products are widely used due to the bioactive components they contain. Honey is one of the important natural products that can be used in the synthesis of silver nanoparticles with its phenolic components and sugars. In this study, the potential of sunflower honey, which is weaker than chestnut and oak honey in terms of bioactive components, for the synthesis of silver nanoparticles was determined. The synthesized sunflower honey-based silver nanoparticles (SH-AgNPs) were characterized and then their antioxidant activity and inhibition of myeloperoxidase and collagenase enzymes, which play an important role in wound healing, were determined. It was determined that the synthesized nanoparticles gave maximum absorbance at 440 nm and particle sizes ranged between 33 nm and 38 nm. The DPPH· radical scavenging activities and ferric reducing capacity (FRAP) of the synthesized nanoparticles were determined as 81±1.42% and 86±1.24%, respectively, and the inhibition properties of myleoperoxidase and collagenase enzymes were determined as 63±1.45% and 37±1.14%, respectively. The findings obtained show that sunflower honey has the potential for use in the field of nanotechnology.

Anahtar Kelimeler

Biological synthesis, Silver nanoparticles, Nanotechnology, Antioxidant activity, Enzyme inhibition

Proje Numarası

1919B012208098 (TÜBİTAK 2209-A)

Kaynakça

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