ESCHERICHIA COLI SUŞLARININ GENOTİP PROFİLLERİNİN PFGE MOLEKÜLER YÖNTEMİ İLE BELİRLENMESİ

Öz

From the family Enterobacteriaceae is Escherichia coli ( E. Coli) is a pathogenic bacterium that is becoming increasingly important, especially in hospitals, common social areas. Thanks to the broad-spectrum beta-lactams (ESBL) they produce, they are usually multi-resistant and are common in intestinal and urinary tract infections. It is of great importance to reduce cross-contamination from public areas and to examine and reveal the genotype structure of the bacteria in question, i.e. to reach fingerprints, subtype sequences of strains. 50 E. Coli strains selected in the study were typified by Pulse field gel electrophoresis.

Anahtar Kelimeler

• Alt Tiplendirme
• Escherichia Coli
• Genotiplendirme
• PFGE

DETERMINATION OF GENOTYPIC PROFILES OF ESCHERICIA COLI STRAINS BY PFGE MOLECULAR METHOD

Öz

From the family Enterobacteriaceae is Escherichia coli ( E. Coli) is a pathogenic bacterium that is becoming increasingly important, especially in hospitals, common social areas. Thanks to the broad-spectrum beta-lactams (ESBL) they produce, they are usually multi-resistant and are common in intestinal and urinary tract infections. It is of great importance to reduce cross-contamination from public areas and to examine and reveal the genotype structure of the bacteria in question, i.e. to reach fingerprints, subtype sequences of strains. 50 E. Coli strains selected in the study were typified by Pulse field gel electrophoresis.

Anahtar Kelimeler

• Escherichia Coli
• Genotyping
• PFGE
• Subtyping

Kaynakça

1. Referans 1: Baştürk, S., Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa ve Acinetobacter baumannii Suşlarında Çeşitli Kinolon 49 Grubu Antibiyotiklerin 63 Duyarlılıklarının Araştırılması, Uzmanlık Tezi, Sağlık Bakanlığı Haseki Eğitim ve Araştırma Hastanesi, İstanbul, 2005.
2. Referans 2: Nameghi., S. A., Genotyping Escherichia coli isolates by Pulsed-Field Gel Electrophoresis, Södertörn Högskola Stockholm,Yüksek lisans tezi. New England BıoLabs, 2007.
3. Refereans 3: Félix, B., Dao, T. T., Lombard, B., Brisabois, A. A. A. and Roussel, S., The Use of Pulsed Field Gel Electrophoresis in Listeria monocytogenes Sub-Typing Harmonization at the European Union Level, Gel Electrophoresis –Principles and Basics (Edited by Magdeldin, S.,), 2012.