Investigation of the Influences of Temperature, Ascorbic Acid and pH on the Kinetics of Degradation of Red Beet ( Beta vulgaris L.) Betacyanins

Yıl 2018, Cilt: 23 Sayı: 2, 217 - 232, 31.08.2018

İşıl Barutçu Mazı Esra Tekin Hasan Türe

https://doi.org/10.17482/uumfd.371118

Cited By: 1

Öz

In this study, the effect of ascorbic acid addition
as stabilizer on
degradation kinetics of betacyanin pigments of red beet (Beta vulgaris L.) extract was investigated under different temperature (4, 25,
40, 60^oC) and pH (5.5,
7.5, 9.5) conditions. In general, although the degradation of betacyanins seems to follow the first-order reaction kinetics
under all investigated conditions, it was determined that the degradation
kinetics of betacyanins shows a change and indicates a better fit to the second
order reaction kinetics at pH 5.5 with the addition of ascorbic acid and at the
temperature of 4°C. It was observed that reaction rate constant (k) values
increased significantly with increasing pH and temperature. k values decreased
significantly while activation energy (Ea) values increased with the addition
of ascorbic acid. Among all results, the lowest k value was reached at 4^oC and pH 5.5 with a value of 0.006×10^-3 min^-1 in the samples with ascorbic acid, while the highest k value was reached at 60^oC
and 9.5 pH with a value of 28.65×10^-3 min^-1 in the
samples without ascorbic acid. Activation energy value ranging between 53.4 and
87.7 kJ/mol was found to be lowest at pH 9.5. The kinetic of color change was
also evaluated using the first order model.

Anahtar Kelimeler

Betacyanin, Red beet, Ascorbic acid, Kinetic

SICAKLIK, ASKORBiK ASiT VE pH’NIN KIRMIZI PANCAR (Beta vulgaris L.) BETASiYANiNLERiNiN BOZUNMA KiNETiĞi ÜZERiNE ETKiLERiNiN iNCELENMESi

Öz

Bu çalışmada farklı
sıcaklık (4,
25, 40, 60^oC) ve pH (5.5,
7.5, 9.5) koşullarında stabilizatör
olarak askorbik asit ilavesinin kırmızı pancar (Beta vulgaris L.) suyu
betasiyanin pigmentlerinin bozunma kinetiği üzerine etkileri incelenmiştir. Betasiyaninlerin
bozunumunun tüm koşullar için birinci dereceden reaksiyon kinetiğini takip
ettiği tespit edilmiştir. Reaksiyon hız sabiti (k) değerlerinin artan pH ve sıcaklık
ile önemli derecede arttığı gözlenmiştir. Askorbik asit ilavesi ile k değerleri
önemli oranda azalırken, aktivasyon enerjisi (Ea) değerleri artmaktadır. Tüm sonuçlar içerisinde en düşük k değerine 4^oC’de
5.5 pH’da askorbik asit ilave edilmiş örneklerde 0.006×10^-3 dak^-1
değeri ile ulaşılırken en yüksek k değerlerine 60^oC’de 9.5
pH’da askorbik asit ilavesiz örneklerde 28.65×10^-3 dak^-1 değeri
ile ulaşılmıştır. 53.4-87.7 kJ/mol arasında değişen aktivasyon enerjisi
değerleri, pH 9.5’ta en düşük bulunmuştur. Ayrıca örneklerin renk değişim
kinetiği de birinci dereceden model kullanılarak incelenmiştir.

Anahtar Kelimeler

Betasiyanin, Kırmızı pancar, Askorbik asit, Kinetik

Kaynakça

• Amjadi, S., Ghorbani, M., Hamishehkar, H. ve Roufegarinejad, L. (2018) Improvement in the stability of betanin by liposomal nanocarriers: its application in gummy candy as a food model, Food Chemistry, 256, 156-162. doi: 10.1016/j.foodchem.2018.02.114
• Antigo, J.L.D., Bergamasco, R.D. ve Madrona, G.S. (2018) Effects of pH on the stability of red beet extract (Beta vulgaris L.) microcapsules produced by spray drying or freeze drying, Food Science and Technology, 38(1), 72-77. doi: 10.1590/1678-457X.34316
• Attia, G.Y., Moussa, M.E.M. ve Sheashea, E.R. (2013) Characterization of red pigments extracted from red beet (Beta vulgaris, L.) and its potential uses as antioxidant and natural food colorants, Egyptian Journal of Agricultural Research, 91 (3), 1095-1110.
• Azeredo, H.M.C. (2009) Betalains: properties, sources, applications, and stability – a review, International Journal of Food Science and Technology, 44 (12), 2365–2376. doi: 10.1111/j.1365-2621.2007.01668.x
• Cai, Y. ve Corke, H. (1999) Amaranthus betacyanin pigments applied in model food systems, Journal of Food Science, 64 (5): 869–873. doi: 10.1111/j.1365-2621.1999.tb15930.x
• Cai, Y., Sun, M., Schliemann, W. ve Corke, H. (2001) Chemical stability and colorant properties of betaxanthin pigments from Celosia argentea, Journal of Agricultural and Food Chemistry, 49 (9), 4429-35. doi: 10.1021/jf0104735
• Chandran, J., Nisha, P., Singhal, R.S. ve Pandit, A.B. (2014) Degradation of colour in beetroot (Beta vulgaris L.): a kinetics study, Journal of Food Science and Technology-Mysore, 51 (10), 2678-2684. doi: 10.1007/s13197-012-0741-9
• Drdak, M. ve Vallova, M. (1990) Kinetics of the thermal degradation of betanine, Nahrung, 34 (4), 307-310. doi: 10.1002/food.19900340402
• EFSA, European Food Safety Authority (2015) Scientific opinion on the re-evaluation of beetroot red (E 162) as a food additive, EFSA Journal, 13 (12), 4318. doi: 10.2903/j.efsa.2015.4318
• Elbandy, M.A. ve Abdelfadeil, M.G. (2008) Stability of betalain pigments from red beetroot (Beta vulgaris), Egyptian Journal of Agricultural Research, 36, 49-60.
• Gandia-Herrero, F., Escribano, J. Ve Garcia-Carmona, F. (2016) Biological activities of plant pigments betalains, Critical Reviews in Food Science and Technology, 56(6), 937-945. doi: 10.1080/10408398.2012.740103
• Gengatharan, A., Dykes, G.A. ve Choo, W.S. (2016) Stability of betacyanin from red pitahaya (Hylocereus polyrhizus) and its potential application as a natural colourant in milk, International Journal of Food Science and Technology, 51(2), 427-434. doi: 10.1111/ijfs.12999
• Gentile, C., Tesoriere, L., Allegra, M., Livrea, M.A. ve D’Alessio, P. (2004) Antioxidant betalains from cactus pear (Opuntia ficus-indica) inhibit endothelial ICAM-1 expression, Signal Transduction and Communication in Cancer Cells, 1028, 481–486. doi: 10.1196/annals.1322.057
• Herbach, K.M., Maier, C., Stintzing, F.C. ve Carle, R. (2007) Effects of processing and storage on juice color and betacyanin stability of purple pitaya (Hylocereus polyrhizus) juice, European Food Research and Technology, 224 (5), 649-658. doi: 10.1007/s00217-006-0354-5
• Herbach, K.M., Rohe, M., Stintzing, F.C. ve Carle, R. (2006a) Structural and chromatic stability of purple pitaya (Hylocereus polyrhizus [Weber] Britton&Rose) betacyanins as affected by the juice matrix and selected additives, Food Research International, 39 (6), 667–677. doi: 10.1016/j.foodres.2006.01.004
• Herbach, K.M., Stintzing, F.C. Carle, R. (2006b) Betalain stability and degradation-structural and chromatic aspects, Journal of Food Science, 71 (4), 41-50. doi: 10.1111/j.1750-3841.2006.00022.x
• Hendry, G.A.F. ve Houghton, J.D. (1996) Natural Food Colourants, Blackie Academic & Professional, London.
• Kanner, J., Harel, S. ve Granit, R. (2001) Betalains - a new class of dietary cationized antioxidants, Journal of Agricultural and Food Chemistry, 49 (11), 5178– 5185. doi: 10.1021/jf010456f
• Kapadia, G.J., Tokuda, H., Konoshima, T. ve Nishino, H. (1996) Chemoprevention of lung and skin cancer by Beta vulgaris (beet) root extract, Cancer Letters, 100 (1-2), 211–214. doi: 10.1016/0304-3835(95)04087-0
• Khan, M.I. (2001) Plant betalains: safety, antioxidant activity, clinical efficacy, and bioavailability, Comprehensive Reviıews in Food Science and Food Safety, 15(2), 316-330. doi: 10.1111/1541- 4337.12185 Kopjar, M., Pilizota, V., Subaric, D. ve Babic, J. (2009) Prevention of thermal degradation of red currant juice anthocyanins by phenolic compounds addition, Croatian Journal of Food Science and Technology, 1 (1), 24-30.
• Labuza, T.P. (1984) Application of chemical kinetics to deterioration of foods, Journal of Chemical Education, 61 (4), 348-358. doi: 10.1021/ed061p348
• Ling, B., Tang, J., Kong, F., Mitcham, E.J. ve Wang, S. (2015) Kinetics of food quality changes during thermal processing: a review, Food and Bioprocess Technology, 8(2), 343-358. doi: 10.1007/s11947-014-1398-3
• Mikolajczyk-Bator, K. ve Czapski, J. (2017) Effect of pH changes on antioxidant capacity and the content of betalain pigments during the heating of a solution of red beet betalains, Polish Journal of Food and Nutrition Science, 67 (2), 123-128. doi: 10.1515/pjfns-2016-0012
• Moldovan, B. ve David, L. (2014). Influence of temperature and preserving agents on the stability of cornelian cherries anthocyanins, Molecules, 19(6), 8177-8188. doi: 10.3390/molecules19068177
• Mosshammer, M.R., Rohe, M., Stintzing, F.C. ve Carle, R. (2007) Stability of yellow-orange cactus pear (Opuntia ficus-indica [L.] Mill. cv. 'Gialla') betalains as affected by the juice matrix and selected food additives, European Food Research and Technology, 225 (1), 21-32. doi: 10.1007/s00217-006-0378-x
• Narkprasom, K., Shao-Pin, W., Shu-Mien, H. ve Pi-Jen, T. (2012) Kinetics of color loss of Djulis (Chenopodium formosanum Koidz.) extracts during storage in different concentrations of alcohol and temperature, APCBEE Procedia, 2, 32-36. doi: 10.1016/j.apcbee.2012.06.007
• Nilsson, T. (1970) Studies into the pigments in beetroot (Beta vulgaris L. ssp. vulgaris var. rubra L.), Lantbrukhogskolans Annaler, 36, 179–219.
• Pedreno, M.A. ve Escribano, J. (2001) Correlation between antiradical activity and stability of betanine from Beta Vulgaris L roots under different pH, temperature and light conditions, Journal of the Science of Food and Agriculture, 81 (7), 627-631. doi: 10.1002/jsfa.851
• Polturak, G. ve Aharoni, A. (2018) "La Vie en Rose'': biosynthesis, sources, and applications of betalain pigments, Molecular Plant, 11(1), 7-22. doi: 10.1016/j.molp.2017.10.008
• Priatni, S. ve Pradita, A. (2015) Stability study of betacyanin extract from red dragon fruit (Hylocereus polyrhizus) peels, Procedia Chemistry, 16, 438-444. doi: 10.1016/j.proche.2015.12.076
• Reddy, M.K., Alexander-Lindo, R.L. ve Nair, M.G. (2005) Relative inhibition of lipid peroxidation, cyclooxygenase enzymes, and human tumor cell proliferation by natural food colors, Journal of Agricultural and Food Chemistry, 53 (23), 9268–9273. doi: 10.1021/jf051399j
• Reshmi, S.K., Aravindhan, K.M. ve Devi, S.P. (2012) The effect of light, temperature, pH on the stability of betacyanin pigments in Basella alba fruit, Asian Journal of Pharmaceutical and Clinical Research, 5 (4), 107-110.
• Roy, K., Gullapalli, S., Chaudhuri, U.R. ve Chakraborty, R. (2004) The use of a natural colorant based on betalain in the manufacture of sweet products in India, International Journal of Food Science and Technology, 39(10), 1087-1091. doi: 10.1111/j.1365-2621.2004.00879.x
• Saguy, I., Kopelman, I.J. ve Mizrahi, S. (1978) Thermal kinetic degradation of betanin and betalamic acid, Journal of Agricultural and Food Chemistry, 26 (2), 360–362. doi: 10.1021/jf60216a052
• Sanchez-Chavez, W., Cortez-Arredondo, J., Solano-Cornejo, M. ve Vidaurre-Ruiz, J. (2015) Kinetics of thermal degradation of betacyanins, betaxantins and vitamin C in a juice–based drink beet (Beta vulgaris l.) and honey, Scientia Agropecuaria, 6(2), 111-118.
• Sapers, G.M. ve Hornstein, J.S. (1979) Varietal differences in colorant properties and stability of red beet pigments, Journal of Food Science, 44 (4), 1245–1248. doi: 10.1111/j.1365-2621.1979.tb03490.x
• Shaaruddin, S., Ghazali, H.M., Mirhosseini, S.H. ve Muhammad, K. (2017) Stability of betanin in pitaya powder and confection as affected by resistant maltodextrin, LWT-Food Science and Technology, 84, 129-134. doi: 10.1016/j.lwt.2017.05.031
• Slimen, I.B., Najar, T., ve Abderrabba, M. (2017) Chemical and antioxidant properties of betalains, Journal of Agricultural and Food Chemistry, 65(4), 675-689. doi: 10.1021/acs.jafc.6b042 08 Stintzing, F.C. ve Carle, R. (2004) Functional properties of anthocyanins and betalains in plants, food, and in human nutrition, Trends in Food Science and Technology, 15 (1), 19–38. doi: 10.1016/j.tifs.2003.07.004
• Stintzing, F.C. ve Carle, R. (2007) Betalains- emerging prospects for food scientists, Trends in Food Science and Technology, 18 (10), 514-525. doi: 10.1016/j.tifs.2007.04.012
• Strack, D., Vogt, T. ve Schliemann, W. (2003) Recent advances in betalain research, Phytochemistry, 62 (3), 247–269. doi: 10.1016/S0031-9422(02)00564-2
• Sumaya-Martínez, M.T., Cruz-Jaime, S., Madrigal-Santillán, E., García-Paredes, J.D., Cariño-Cortés, R., Cruz-Cansino, N., Valadez-Veg,a C., Martinez-Cardenas, L. ve Alanís-García, E. (2011) Betalain, acid ascorbic, phenolic contents and antioxidant properties of purple, red, yellow and white cactus pears, International Journal of Molecular Sciences, 12 (10), 6452-6468. doi: 10.3390/ijms12106452
• Tangüler, H. (2010) Şalgam suyu üretiminde etkili olan laktik asit bakterilerinin belirlenmesi ve şalgam suyu üretim tekniğinin geliştirilmesi, Doktora Tezi, Ç. Ü. Fen Bilimleri Enstitüsü, Adana.
• Von Elbe, J.H. ve Maing, I.Y. (1973) Betalains as possible food colorants of meat substitutes, Cereal Science Today, 18 (9), 263–4, 316–7.
• Von Elbe, J.H., Maing, I. ve Amundson, C.H. (1974) Colour stability of betanin, Journal of Food Science, 39 (2), 334–337. doi: 10.1111/j.1365-2621.1974.tb02888.x
• Woo, K.K., Ngou, F.H., Ngo, L.S., Soong, W.K. ve Tang, P.Y. (2011) Stability of betalain pigment from red dragon fruit (Hylocereus polyrhizus), American Journal of Food Technology, 6 (2), 140-148. doi: 10.3923/ajft.2011.140.148
• Yıldız Turp, G., Kazan, H. Ve Ünübol, H. (2016) Sosis üretiminde doğal renk maddesi ve antioksidan olarak kırmızı