Deneysel Kafa Travması Geçiren Sıçanlarda Erken Dönemde Serum Nörogranin ve PNF-H Düzeyi

Öz

Deneysel olarak hafif travmatik beyin hasarı oluşturulmuş sıçanlardan alınan serum örneklerinde, yaralanmadan sonraki ilk beş saat içinde Nörogranin ve Fosforile Nörofilament Ağır Zincirinin seyri incelenmiştir. Bu deneysel hayvan çalışmasının temelini 32 dişi Spraque-Dawley sıçanı oluşturmuştur. Her gruptaki sıçan sayısı toplam 8'dir (n=8), 3 deney grubu ve 1 kontrol grubu. Sıçanlarda 80 cm yükseklikten düşürülen 50 mg'lık metal bir yapı ile travmatik beyin hasarı oluşturulmuştur. Travmadan 1, 3 ve 5 saat sonra sıçanlardan alınan serum örneklerindeki Nörogranin ve Fosforile Nörofilament Ağır Zinciri oranları kontrol grubu ile karşılaştırılmıştır. Travmatik beyin hasarı sonrası sıçanlardan alınan serum örneklerinden ölçülen nörogranin değerlerinin 3. ve 5. saatlerdeki değerlerinin kontrol grubuna göre istatistiksel olarak daha yüksek olduğu belirlendi. 3. ve 5. saat değerleri karşılaştırıldığında, 5. saat değerinin 3. saat değerinden daha düşük olduğu tespit edildi. pNF-H seviyesi açısından incelendiğinde, 1. ve 3. saatlerde anlamlı derecede yüksek olduğu, 5. saatte ise anlamlı bir düşüş gözlendiği görüldü. Serum nörogranin ve pNF-H seviyelerinin artırılması, hafif travmatik beyin hasarı olan hastaların tanı ve tedavisinde faydalı olabilir, ancak insanlarda faydasını belirlemek için daha kapsamlı çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

• Hafif Travmatik Beyin Hasarı
• Nörogranin
• Fosforile Nörofilament Ağır Zinciri.

Serum Neurogranin and PNF-H Level in the Early Period in Rats with Experimental Head Trauma

Öz

This study investigates the course of Neurogranin and Phosphorylated Neurofilament Heavy Chain in serum samples taken from rats experimentally induced with mild traumatic brain injury during the first five hours after injury. This experimental animal study was based on 32 female Sprague-Dawley rats. Each group consisted of 8 rats (n=8), 3 experimental groups and 1 control group. Traumatic brain injury was induced in the rats by dropping a 50 mg metal object from a height of 80 cm. Neurogranin and Phosphorylated Neurofilament Heavy Chain ratios in serum samples taken from the rats 1, 3, and 5 hours after trauma were compared with the control group. It was determined that the neurogranin values measured in serum samples taken from rats after traumatic brain injury were statistically higher at 3 and 5 hours compared to the control group. Comparing the 3rd and 5th hour values, it was found that the 5th hour value was lower than the 3rd hour value. Regarding pNF-H levels, it was observed to be significantly higher at 1 and 3 hours, and a significant decrease at 5 hours. Increasing serum neurogranin and pNF-H levels may be beneficial in the diagnosis and treatment of patients with mild traumatic brain injury, but more comprehensive studies are needed to determine its benefit in humans.

Anahtar Kelimeler

• Mild Traumatic Brain Injury
• Neurogranin
• Phosphorylated Neurofilament Heavy Chain.

Kaynakça

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