EN
TR
Comparison of HPLC, CMIA and ECLIA Methods for Measurement of Plasma 25-OH Vitamin D
Öz
The aim of the study is to compare two automated chemiluminescent immunassays [Chemiluminesence immunoassay (CMIA) and electro-chemiluminescent immunoassay (ECLIA)] with the high performance liquid chromatography (HPLC) for quantification of plasma 25-OH vitamin D. Blood samples from 121 consecutive patients were collected and analyzed for the method comparison. Mean 25-OH vitamin D levels obtained by the HPLC method were found to be higher than the other two methods. However, statistically significant difference was found only with ECLIA (p=0.011). The three methods were compared by linear regression and the highest correlation was found for HPLC and ECLIA (r = 0.869), intermediate for CMIA-ECLIA (r = 0.818) and lowest for HPLC-CMIA (r = 0.779). As a result, the higher 25-OH vitamin D values were obtained with HPLC method, but the three methods showed good correlation and agreed well with each other. So that preferring the chemiluminescence methods which can be used in automated systems, seems to be advantageous.
Anahtar Kelimeler
Ayrıntılar
Birincil Dil
İngilizce
Konular
-
Bölüm
-
Yayımlanma Tarihi
1 Haziran 2015
Gönderilme Tarihi
1 Haziran 2015
Kabul Tarihi
-
Yayımlandığı Sayı
Yıl 2015 Cilt: 41 Sayı: 2
APA
Ustaalioğlu, Y. E., & Dirican, M. (2015). Comparison of HPLC, CMIA and ECLIA Methods for Measurement of Plasma 25-OH Vitamin D. Journal of Uludağ University Medical Faculty, 41(2), 53-58. https://izlik.org/JA58CB76HD
AMA
1.Ustaalioğlu YE, Dirican M. Comparison of HPLC, CMIA and ECLIA Methods for Measurement of Plasma 25-OH Vitamin D. Uludağ Tıp Derg. 2015;41(2):53-58. https://izlik.org/JA58CB76HD
Chicago
Ustaalioğlu, Yunus Emre, ve Melahat Dirican. 2015. “Comparison of HPLC, CMIA and ECLIA Methods for Measurement of Plasma 25-OH Vitamin D”. Journal of Uludağ University Medical Faculty 41 (2): 53-58. https://izlik.org/JA58CB76HD.
EndNote
Ustaalioğlu YE, Dirican M (01 Haziran 2015) Comparison of HPLC, CMIA and ECLIA Methods for Measurement of Plasma 25-OH Vitamin D. Journal of Uludağ University Medical Faculty 41 2 53–58.
IEEE
[1]Y. E. Ustaalioğlu ve M. Dirican, “Comparison of HPLC, CMIA and ECLIA Methods for Measurement of Plasma 25-OH Vitamin D”, Uludağ Tıp Derg, c. 41, sy 2, ss. 53–58, Haz. 2015, [çevrimiçi]. Erişim adresi: https://izlik.org/JA58CB76HD
ISNAD
Ustaalioğlu, Yunus Emre - Dirican, Melahat. “Comparison of HPLC, CMIA and ECLIA Methods for Measurement of Plasma 25-OH Vitamin D”. Journal of Uludağ University Medical Faculty 41/2 (01 Haziran 2015): 53-58. https://izlik.org/JA58CB76HD.
JAMA
1.Ustaalioğlu YE, Dirican M. Comparison of HPLC, CMIA and ECLIA Methods for Measurement of Plasma 25-OH Vitamin D. Uludağ Tıp Derg. 2015;41:53–58.
MLA
Ustaalioğlu, Yunus Emre, ve Melahat Dirican. “Comparison of HPLC, CMIA and ECLIA Methods for Measurement of Plasma 25-OH Vitamin D”. Journal of Uludağ University Medical Faculty, c. 41, sy 2, Haziran 2015, ss. 53-58, https://izlik.org/JA58CB76HD.
Vancouver
1.Yunus Emre Ustaalioğlu, Melahat Dirican. Comparison of HPLC, CMIA and ECLIA Methods for Measurement of Plasma 25-OH Vitamin D. Uludağ Tıp Derg [Internet]. 01 Haziran 2015;41(2):53-8. Erişim adresi: https://izlik.org/JA58CB76HD
