Investigation of Anticytotoxic and Antigenotoxic Effect of Rosmarinic Acid Against Cisplatın In A549 and Beas-2B Cell Lines

Öz

Rosmarinic Acid (RA) is an important phenolic compound found in many plant species. The aim of this study was to determine the efficacy of RA against  Cisplatin-induced cytotoxic, genotoxic effects and oxidative stress in A549 (Human Lung Cancer Cell Line) and Beas-2B (Human Healthy Bronchial Epithelial Cell Line) cells. In this way, it was aimed to determine the possible effects of RA-containing herbal products that can be used by patients, on Cisplatin treatment during Cisplatin chemotherapy. For this purpose, XTT assay was used to determine cytotoxicity, Comet  assay was used to determine genotoxicity and,  DCFH-DA assay was used to determine oxidative stress values. Cells were exposed to two different doses of Cisplatin (11, 22 µM) for 24 hours. Cells were exposed to three different concentrations of RA (50, 100, 200 µM) both alone and in combination with two different doses of Cisplatin for 24 hours. As a result, it was determined, used RA concentrations have anti cytotoxic activity potential, against the cytotoxic effect increased by Cisplatin and antigenotoxic activity potential, against the genotoxic effect increased by Cisplatin. It was found that these activities of RA were based on antioxidant properties which reduced oxidative stress parameters increased by Cisplatin. In conclusion, the necessity to prevent the unconscious consumption of herbal products containing RA, especially in patients receiving Cisplatin chemotherapy has been shown.

Anahtar Kelimeler

• Rosmarinic acid,Cisplatin,Anticytotoxicity,Antigenotoxicity

Rosmarinik Asidin Cisplatine Karşı Antisitotoksik ve Antigenotoksik Etkisinin A549 ve Beas-2B Hücre Hatlarında Araştırılması

Öz

Rosmarinik Asit (RA) birçok bitki türünde bulunan önemli bir fenolik bileşiktir. Çalışmada RA’nın, A549 (İnsan Akciğer Kanser Hücre Hattı) ve Beas-2B (İnsan Sağlıklı Bronşial Epitel Hücre Hattı)  hücrelerinde, Cisplatin tarafından oluşturulan sitotoksik, genotoksik etki ve oksidatif strese karşın etkinliğinin in vitro olarak belirlenmesi ve böylelikle Cisplatin kemoterapisi sırasında hastalar tarafından kullanılabilecek RA içerikli bitkisel ürünlerin Cisplatin tedavisi üzerindeki olası etkilerinin belirlenmesi amaçlanmıştır. Bu amaca yönelik olarak sitotoksite belirlenmesinde XTT, genotoksite belirlenmesinde Komet ve oksidatif stres değerlerinin belirlenmesinde ise DCFH-DA yöntemleri kullanılmıştır. Yöntemlerde hücreler Cisplatin’in iki farklı dozuna (11, 22 µM) 24 saat süre ile maruz bırakılmıştır. Hücreler RA’nın üç farklı konsantrasyonuna (50, 100, 200 µM) hem tek başına hem de Cisplatin’in iki farklı dozu ile beraber uygulanarak 24 saat süre ile maruz bırakılmıştır. Sonuç olarak Cisplatin dozları tarafından arttırılan; sitotoksik etkiye karşın RA’nın kullanılan konsantrasyonlarının antisitotoksik aktivite, genotoksik etkiye karşın ise antigenotoksik aktivite gösterme potansiyeli olduğu belirlenmiştir. RA’nın bu aktivitelerinin temelinde de Cisplatin tarafından arttırılan oksidatif stres parametrelerini azaltıcı antioksidan özellik göstermesi olduğu belirlenmiştir. Sonuç olarak RA içeren bitkisel ürünlerin, özellikle Cisplatin kemoterapisi alan hastalarda bilinçsizce tüketiminin önlenmesi gerekliliği gösterilmiştir.

Anahtar Kelimeler

• Rosmarinik asit,Cisplatin,Antisitotoksite,Antigenotoksite

Kaynakça

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